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The map label for this gene is gcvT

Identifier: 85059980

GI number: 85059980

Start: 3458150

End: 3459250

Strand: Reverse

Name: gcvT

Synonym: SG2002

Alternate gene names: 85059980

Gene position: 3459250-3458150 (Counterclockwise)

Preceding gene: 85059981

Following gene: 85059979

Centisome position: 82.93

GC content: 60.22

Gene sequence:

>1101_bases
ATGGCTCAGCAGACCCCGCTGTATGAAGAGCACCACGCGAGCGGCGCCAAAATGGTGGATTTCCACGGCTGGATGATGCC
GCTTCACTATGGTTCCCAACTGGATGAACATCATCAGGTGCGGCGCGATGTCGGCATGTTTGACGTGTCGCACATGACCA
TTGTCGATTTAGCCGGACCGCGCACCCGCGATTTTCTTCGCCATCTGCTGGCCAACGATGTTGCCAAGCTGACGGTGCCC
GGCAAAGCGCTTTACACCGGGATGCTTAATGCGTCCGGTGGCGTGATAGACGACCTTATCGTCTATTTCCTTTCCGAAAT
TGAATTCCGCCTGGTTGTCAACTCTGAGACACGTGATAAAGACGTTGAGTGGATCACCCGCCACGCCGAGCCGTATCAGG
TAACGGTGACCGTGCGCGACGATCTGGCGCTGATAGCGGTGCAGGGGCCGCAGGCGCAAAGCAAGACGCAGACGCTATTC
AGCTCGCAACAGCGGCAGGCGGTAAGCGGAATGAAACCGTTTTTTGGCGTTCAGGCCAACGAGTTGTTTATTGCCACCAC
CGGCTATACTGGCGAGGCTGGCTACGAGATTGCGTTACCGGCAACCCAGGCCGCCGATTTCTGGCGGCAACTGCTGGCGG
CCGGCGTGAAGCCCTGCGGCCTCGGCGCGCGCGACACCCTGCGCCTGGAAGCGGGGATGAACCTTTACGGTCAGGAAATG
GATGAGGGGATCTCTCCGCTCGCGGCCAATATGGGCTGGACCATCACTTGGCGGCCGGAGGGGCGCGATTTCATCGGACG
CGAGGCGCTGGAGGCCCAGCGCGCCGCCGGCGACGGCGAGCAATTGGTCGGGTTGGTGATGACTGAAAAAGGCGTATTGC
GCAACGGCCTGCCAGTCCATTTCACCGACGCTGCCGGTAACATGCAGCAAGGGATCATCACCAGCGGCTCGTTTTCGCCG
ACGCTGGGCGTCAGCATTGCACTGGCGCGGGTGCCGGCCGGTATCGGCCAGCAGGCGATAGTTTTGATCCGTAACCGCGA
AATGCCGGTTAAGGTCACCAAACCCGGTTTTGTGCGCGCCGGTAAAGCGGTTGCGCAATAA

Upstream 100 bases:

>100_bases
CCCAGAAGGTATCGTTTGCGTCCGCCCTCATTACCCGCTCGGGCAAGGGGTTTGCGCGCTTCGGTAATTATCTGTCTTCC
CATTGAGAAAGAGAGAAAAG

Downstream 100 bases:

>100_bases
CTAACTTTGTCAGAGTGAGGATGGCGATGAGCAATGTGCCGACAGAATTAAAATATACGGCATCCCATGAGTGGGTGCTT
AATGAAGGCGATGGTGTTTA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAQQTPLYEEHHASGAKMVDFHGWMMPLHYGSQLDEHHQVRRDVGMFDVSHMTIVDLAGPRTRDFLRHLLANDVAKLTVP
GKALYTGMLNASGGVIDDLIVYFLSEIEFRLVVNSETRDKDVEWITRHAEPYQVTVTVRDDLALIAVQGPQAQSKTQTLF
SSQQRQAVSGMKPFFGVQANELFIATTGYTGEAGYEIALPATQAADFWRQLLAAGVKPCGLGARDTLRLEAGMNLYGQEM
DEGISPLAANMGWTITWRPEGRDFIGREALEAQRAAGDGEQLVGLVMTEKGVLRNGLPVHFTDAAGNMQQGIITSGSFSP
TLGVSIALARVPAGIGQQAIVLIRNREMPVKVTKPGFVRAGKAVAQ

Sequences:

>Translated_366_residues
MAQQTPLYEEHHASGAKMVDFHGWMMPLHYGSQLDEHHQVRRDVGMFDVSHMTIVDLAGPRTRDFLRHLLANDVAKLTVP
GKALYTGMLNASGGVIDDLIVYFLSEIEFRLVVNSETRDKDVEWITRHAEPYQVTVTVRDDLALIAVQGPQAQSKTQTLF
SSQQRQAVSGMKPFFGVQANELFIATTGYTGEAGYEIALPATQAADFWRQLLAAGVKPCGLGARDTLRLEAGMNLYGQEM
DEGISPLAANMGWTITWRPEGRDFIGREALEAQRAAGDGEQLVGLVMTEKGVLRNGLPVHFTDAAGNMQQGIITSGSFSP
TLGVSIALARVPAGIGQQAIVLIRNREMPVKVTKPGFVRAGKAVAQ
>Mature_365_residues
AQQTPLYEEHHASGAKMVDFHGWMMPLHYGSQLDEHHQVRRDVGMFDVSHMTIVDLAGPRTRDFLRHLLANDVAKLTVPG
KALYTGMLNASGGVIDDLIVYFLSEIEFRLVVNSETRDKDVEWITRHAEPYQVTVTVRDDLALIAVQGPQAQSKTQTLFS
SQQRQAVSGMKPFFGVQANELFIATTGYTGEAGYEIALPATQAADFWRQLLAAGVKPCGLGARDTLRLEAGMNLYGQEMD
EGISPLAANMGWTITWRPEGRDFIGREALEAQRAAGDGEQLVGLVMTEKGVLRNGLPVHFTDAAGNMQQGIITSGSFSPT
LGVSIALARVPAGIGQQAIVLIRNREMPVKVTKPGFVRAGKAVAQ

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=370, Percent_Identity=33.2432432432432, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI257796258, Length=352, Percent_Identity=33.2386363636364, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI257796254, Length=365, Percent_Identity=30.1369863013699, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI257796256, Length=307, Percent_Identity=31.5960912052117, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI24797151, Length=359, Percent_Identity=28.133704735376, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI197927446, Length=323, Percent_Identity=25.6965944272446, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI21361378, Length=323, Percent_Identity=25.6965944272446, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789272, Length=365, Percent_Identity=79.7260273972603, Blast_Score=601, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17560118, Length=375, Percent_Identity=33.0666666666667, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32563613, Length=348, Percent_Identity=22.4137931034483, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=335, Percent_Identity=23.2835820895522, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994052, Length=335, Percent_Identity=23.2835820895522, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320222, Length=378, Percent_Identity=33.3333333333333, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI20129441, Length=378, Percent_Identity=32.8042328042328, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28571104, Length=270, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI20130091, Length=428, Percent_Identity=24.2990654205607, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_SODGM (Q2NRE8)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455682.1
- HSSP:   P27248
- ProteinModelPortal:   Q2NRE8
- SMR:   Q2NRE8
- STRING:   Q2NRE8
- GeneID:   3867083
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2002
- NMPDR:   fig|343509.6.peg.4330
- eggNOG:   COG0404
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- PhylomeDB:   Q2NRE8
- ProtClustDB:   PRK00389
- BioCyc:   SGLO343509:SG2002-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 39916; Mature: 39785

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQTPLYEEHHASGAKMVDFHGWMMPLHYGSQLDEHHQVRRDVGMFDVSHMTIVDLAGP
CCCCCCCCHHHCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHCCCEEECCEEEEECCCC
RTRDFLRHLLANDVAKLTVPGKALYTGMLNASGGVIDDLIVYFLSEIEFRLVVNSETRDK
CHHHHHHHHHHCCCEEEEECCHHHEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
DVEWITRHAEPYQVTVTVRDDLALIAVQGPQAQSKTQTLFSSQQRQAVSGMKPFFGVQAN
CHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
ELFIATTGYTGEAGYEIALPATQAADFWRQLLAAGVKPCGLGARDTLRLEAGMNLYGQEM
EEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHH
DEGISPLAANMGWTITWRPEGRDFIGREALEAQRAAGDGEQLVGLVMTEKGVLRNGLPVH
HCCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHCCCCEE
FTDAAGNMQQGIITSGSFSPTLGVSIALARVPAGIGQQAIVLIRNREMPVKVTKPGFVRA
EECCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCC
GKAVAQ
CHHHCC
>Mature Secondary Structure 
AQQTPLYEEHHASGAKMVDFHGWMMPLHYGSQLDEHHQVRRDVGMFDVSHMTIVDLAGP
CCCCCCCHHHCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHCCCEEECCEEEEECCCC
RTRDFLRHLLANDVAKLTVPGKALYTGMLNASGGVIDDLIVYFLSEIEFRLVVNSETRDK
CHHHHHHHHHHCCCEEEEECCHHHEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
DVEWITRHAEPYQVTVTVRDDLALIAVQGPQAQSKTQTLFSSQQRQAVSGMKPFFGVQAN
CHHHHHCCCCCEEEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
ELFIATTGYTGEAGYEIALPATQAADFWRQLLAAGVKPCGLGARDTLRLEAGMNLYGQEM
EEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHH
DEGISPLAANMGWTITWRPEGRDFIGREALEAQRAAGDGEQLVGLVMTEKGVLRNGLPVH
HCCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEEECCCHHHCCCCEE
FTDAAGNMQQGIITSGSFSPTLGVSIALARVPAGIGQQAIVLIRNREMPVKVTKPGFVRA
EECCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEECCCCCCCC
GKAVAQ
CHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA