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The map label for this gene is hda [H]

Identifier: 85059708

GI number: 85059708

Start: 2944536

End: 2945225

Strand: Reverse

Name: hda [H]

Synonym: SG1730

Alternate gene names: 85059708

Gene position: 2945225-2944536 (Counterclockwise)

Preceding gene: 85059709

Following gene: 85059704

Centisome position: 70.61

GC content: 55.94

Gene sequence:

>690_bases
GTGCAGCTTTCTTTACCGCTCTATTTACCCGATGACGAAACTTTCGCCAGCTTTTATCCTGGCGAAAATGGCGCGTTGCT
GGCGGCGTTGCAGGCTATGCTGGACCACGAACACGGCAGCTACCTTTATTTCTGGTCGCGTAAGGGTGGTGGCCGCAGCC
ATTTGCTGCATGCCGCCTGCGCGGCGCTATCGGCGCGCGCAAAGGCCGTAGGTTATGTGCCGCTGGACAAGCGAACCTGG
TTTGTTCCAGAGGTGCTGGAGGGCATGGAGCAACTGGCGCTGGTGACGATGGATAATATCGAATGTATCGTCGGCGACCT
GCCGTGGGAAATGGCTATTTTCAACCTCTATAACCGCATTCAGGAAACCGGGCGCACCCGGCTTATTATCAGCGGCAATT
GTCCGCCCCGCCAATTGAATCTCAGCTTGCCAGATTTGGCCTCGCGCCTAGATTGGGGGCAAATTTATCGCCTGCAGCCG
CTTTCCGATGAGGAAAAGGGCTATGCCTTGCAAATGCGGGCGAAACTGCGCGGCTTCGAATTGCCGGAGGATGTCAGCCG
TTTCTTGCTGAAAAGATTGGAAAGGGATATGCGCACCTTGTCGGCGACGCTGGATCAGCTGGACCATGCCTCTATCCGCG
CCCAGCGCAAACTGACCATTCCCTTCGTGAAAGCGATTCTGAACCTTTAG

Upstream 100 bases:

>100_bases
CTCCTGATGGCCGTACCGCGCCGTCCTCGCGGCGCGGTAAAGTTATGGTAAACTGGAAAGGATCTTTGCCAGAACGCAAG
AGGTACATCTGAACACGCCG

Downstream 100 bases:

>100_bases
GGGGTACGCCTTATAACCCTGTATCGGCAGGATGAGCGCTGCGGGCGGCTTATATTACCTATTCCGTGCAGGGCTGACGC
TGAACTCTCCCTGCGTTACT

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MQLSLPLYLPDDETFASFYPGENGALLAALQAMLDHEHGSYLYFWSRKGGGRSHLLHAACAALSARAKAVGYVPLDKRTW
FVPEVLEGMEQLALVTMDNIECIVGDLPWEMAIFNLYNRIQETGRTRLIISGNCPPRQLNLSLPDLASRLDWGQIYRLQP
LSDEEKGYALQMRAKLRGFELPEDVSRFLLKRLERDMRTLSATLDQLDHASIRAQRKLTIPFVKAILNL

Sequences:

>Translated_229_residues
MQLSLPLYLPDDETFASFYPGENGALLAALQAMLDHEHGSYLYFWSRKGGGRSHLLHAACAALSARAKAVGYVPLDKRTW
FVPEVLEGMEQLALVTMDNIECIVGDLPWEMAIFNLYNRIQETGRTRLIISGNCPPRQLNLSLPDLASRLDWGQIYRLQP
LSDEEKGYALQMRAKLRGFELPEDVSRFLLKRLERDMRTLSATLDQLDHASIRAQRKLTIPFVKAILNL
>Mature_229_residues
MQLSLPLYLPDDETFASFYPGENGALLAALQAMLDHEHGSYLYFWSRKGGGRSHLLHAACAALSARAKAVGYVPLDKRTW
FVPEVLEGMEQLALVTMDNIECIVGDLPWEMAIFNLYNRIQETGRTRLIISGNCPPRQLNLSLPDLASRLDWGQIYRLQP
LSDEEKGYALQMRAKLRGFELPEDVSRFLLKRLERDMRTLSATLDQLDHASIRAQRKLTIPFVKAILNL

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=228, Percent_Identity=77.6315789473684, Blast_Score=366, Evalue=1e-103,
Organism=Escherichia coli, GI2367267, Length=229, Percent_Identity=25.3275109170306, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 26002; Mature: 26002

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSLPLYLPDDETFASFYPGENGALLAALQAMLDHEHGSYLYFWSRKGGGRSHLLHAAC
CCEECEEECCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
AALSARAKAVGYVPLDKRTWFVPEVLEGMEQLALVTMDNIECIVGDLPWEMAIFNLYNRI
HHHHHHHHEEEEEECCCCCEEHHHHHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHH
QETGRTRLIISGNCPPRQLNLSLPDLASRLDWGQIYRLQPLSDEEKGYALQMRAKLRGFE
HHCCCEEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHCCCC
LPEDVSRFLLKRLERDMRTLSATLDQLDHASIRAQRKLTIPFVKAILNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MQLSLPLYLPDDETFASFYPGENGALLAALQAMLDHEHGSYLYFWSRKGGGRSHLLHAAC
CCEECEEECCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
AALSARAKAVGYVPLDKRTWFVPEVLEGMEQLALVTMDNIECIVGDLPWEMAIFNLYNRI
HHHHHHHHEEEEEECCCCCEEHHHHHHHHHHHHHHEECCCEEEECCCCHHHHHHHHHHHH
QETGRTRLIISGNCPPRQLNLSLPDLASRLDWGQIYRLQPLSDEEKGYALQMRAKLRGFE
HHCCCEEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCCCCCEEEEEEHHHCCCC
LPEDVSRFLLKRLERDMRTLSATLDQLDHASIRAQRKLTIPFVKAILNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA