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The map label for this gene is cysW

Identifier: 85059685

GI number: 85059685

Start: 2917704

End: 2918573

Strand: Reverse

Name: cysW

Synonym: SG1707

Alternate gene names: 85059685

Gene position: 2918573-2917704 (Counterclockwise)

Preceding gene: 85059686

Following gene: 85059684

Centisome position: 69.97

GC content: 59.31

Gene sequence:

>870_bases
ATGACAGATTTCTCCGTGTTCAACGGCGCCGCGCGCGCGCCTTTTCCCTGGGGAAAATGGCTATTGATCGGCCTCGGCTC
AGTGGTATCGCTGCTGTTGCTGGTGGTGCCGCTGATTTCAATTTTCGCCACCGCACTGGCGGAAGGACTGGCGGCGGTGG
GGCGCAATCTCAACGATAGCGACATGCTGCACGCCATTTGGCTGACGGTGCTCATCGCGCTGATAACCGTACCGGTGAAT
GTGGTGTTCGGCACCTTAATGGCCTGGCTGGTGACGCGCTTCAATTTTCGCGGCCGCCAGCTATTGCTTACGCTGATGGA
TGTGCCTTTCGCGGTATCGCCCGTGGTGGCGGGATTGCTATACCTCCTGTTTTACGGGACTAACGGCCCCCTCGGAGGCT
GGCTGGACGAGCATAATATGCAGTTGATGTTCTCATGGCCGGGGATGGTGCTGGTGACGATATTTGTTACCTGTCCGTTT
GTGGTGCGCGAATTGGTGCCGGTGATGCTGAGCCAGGGCAGCCAGGAGGACGAGGCCGCGATCCTGTTGGGTGCATCCAG
TTGGCAGATGTTTCGCCGCGTCACGTTGCCCAACATCTACTGGGCGCTGCTGTATGGCGTGATCCTGACCAACGCCCGCG
CAATCGGCGAGTTCGGCGCGGTATCGGTGGTATCGGGGTCTATCCGCGGCGAAACCTATACCCTGCCGCTGCAGGTGGAA
TTACTGCATCAGGATTACAACACCGTGGGCGCGTTTACCGCCGCCGCGCTGCTGACTCTGATGGCGATTGTGACATTATT
CTTGAAGAGCGGGCTGCAGTGGCGTCTGGCGCGCCATCAGGCAGAGCTTCAACCGGGGGACACACAATGA

Upstream 100 bases:

>100_bases
CCAGCGCTATCGCCTCGGTAATCCTGGTGGCGTCGCTGCTGCTGCTCTTCGCCATTAACGTGCTGCAATCCCGCTTCGGC
CGTCGGTTGGGAGGACAATG

Downstream 100 bases:

>100_bases
GTATAGCGATCGATAAGGTCGGCAAGTTTTTTGCCAACAGCAAAGTATTGAACGATATTTCGCTGGATATCGCCTCCGGG
GAAATGGTCGTGCTGCTGGG

Product: sulfate/thiosulfate transporter permease subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTDFSVFNGAARAPFPWGKWLLIGLGSVVSLLLLVVPLISIFATALAEGLAAVGRNLNDSDMLHAIWLTVLIALITVPVN
VVFGTLMAWLVTRFNFRGRQLLLTLMDVPFAVSPVVAGLLYLLFYGTNGPLGGWLDEHNMQLMFSWPGMVLVTIFVTCPF
VVRELVPVMLSQGSQEDEAAILLGASSWQMFRRVTLPNIYWALLYGVILTNARAIGEFGAVSVVSGSIRGETYTLPLQVE
LLHQDYNTVGAFTAAALLTLMAIVTLFLKSGLQWRLARHQAELQPGDTQ

Sequences:

>Translated_289_residues
MTDFSVFNGAARAPFPWGKWLLIGLGSVVSLLLLVVPLISIFATALAEGLAAVGRNLNDSDMLHAIWLTVLIALITVPVN
VVFGTLMAWLVTRFNFRGRQLLLTLMDVPFAVSPVVAGLLYLLFYGTNGPLGGWLDEHNMQLMFSWPGMVLVTIFVTCPF
VVRELVPVMLSQGSQEDEAAILLGASSWQMFRRVTLPNIYWALLYGVILTNARAIGEFGAVSVVSGSIRGETYTLPLQVE
LLHQDYNTVGAFTAAALLTLMAIVTLFLKSGLQWRLARHQAELQPGDTQ
>Mature_288_residues
TDFSVFNGAARAPFPWGKWLLIGLGSVVSLLLLVVPLISIFATALAEGLAAVGRNLNDSDMLHAIWLTVLIALITVPVNV
VFGTLMAWLVTRFNFRGRQLLLTLMDVPFAVSPVVAGLLYLLFYGTNGPLGGWLDEHNMQLMFSWPGMVLVTIFVTCPFV
VRELVPVMLSQGSQEDEAAILLGASSWQMFRRVTLPNIYWALLYGVILTNARAIGEFGAVSVVSGSIRGETYTLPLQVEL
LHQDYNTVGAFTAAALLTLMAIVTLFLKSGLQWRLARHQAELQPGDTQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=276, Percent_Identity=79.3478260869565, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1788764, Length=223, Percent_Identity=36.7713004484305, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=32.2869955156951, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31623; Mature: 31492

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSVFNGAARAPFPWGKWLLIGLGSVVSLLLLVVPLISIFATALAEGLAAVGRNLNDS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DMLHAIWLTVLIALITVPVNVVFGTLMAWLVTRFNFRGRQLLLTLMDVPFAVSPVVAGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
YLLFYGTNGPLGGWLDEHNMQLMFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAA
HHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILLGASSWQMFRRVTLPNIYWALLYGVILTNARAIGEFGAVSVVSGSIRGETYTLPLQVE
EEEECHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHHEECCCCCCEEEEEEEHH
LLHQDYNTVGAFTAAALLTLMAIVTLFLKSGLQWRLARHQAELQPGDTQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDFSVFNGAARAPFPWGKWLLIGLGSVVSLLLLVVPLISIFATALAEGLAAVGRNLNDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DMLHAIWLTVLIALITVPVNVVFGTLMAWLVTRFNFRGRQLLLTLMDVPFAVSPVVAGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
YLLFYGTNGPLGGWLDEHNMQLMFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAA
HHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILLGASSWQMFRRVTLPNIYWALLYGVILTNARAIGEFGAVSVVSGSIRGETYTLPLQVE
EEEECHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCHHHEECCCCCCEEEEEEEHH
LLHQDYNTVGAFTAAALLTLMAIVTLFLKSGLQWRLARHQAELQPGDTQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]