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The map label for this gene is cysU [H]

Identifier: 85059686

GI number: 85059686

Start: 2918573

End: 2919406

Strand: Reverse

Name: cysU [H]

Synonym: SG1708

Alternate gene names: 85059686

Gene position: 2919406-2918573 (Counterclockwise)

Preceding gene: 85059687

Following gene: 85059685

Centisome position: 69.99

GC content: 61.03

Gene sequence:

>834_bases
ATGCTGGTCCTGACCACCAAACGGGTCCTGCCCGGTTTTAGCCTGAGCCTCGGCGGCAGCGTGCTGTTCGTTTGCTTGAT
CCTGCTGCTGCCGTTAAGCGCGCTGGTTATGCAGTTAGCGCAGATGAGTTGGTCACAATACTGGGAGGTCATCACCGGCC
CGGAGCTGTTGGCGGCCTATAAAATCACGCTGATCTGCGCAGGGGTGGCGTCGTTTTTCAATGCGGTGTTCGGCATGCTG
ATGGCCTGGATCCTGACCCGCTATCAATTTCCCGGCCGTGCGCTGCTGGACGGCCTGATGGATCTACCGTTCGCCCTGCC
TACCGCGGTGGCAGGTTTGACGCTGGCCACGCTATTTTCCACCACCGGCTGGTACGGCGGCTGTCTGGCCCAGTTTGACA
TTAAAGTCTCCTACACCTGGCTCGGTATCGCCGTGGCGATGGCCTTCACCAGCATCCCGTTCGTGGTGCGTACCGTGCAG
CCGGTGCTGGAAGAGCTGGGGCCGGAATACGAGGAAGCGGCCCAAACGCTCGGCGCCGGGCACTGGCAGACCTTTCGCCG
CGTTATTCTGCCGGAAGTTGCGCCGGCGCTGCTGGCGGGGACCGCGCTGTCGTTCACCCGCAGCCTGGGCGAATTCGGCG
CGGTGATCTTTATCGCCGGCAATATCGCCTGGAAAACCGAGGTCACCTCTTTAATGATTTTTATCCGCCTGCAGGAGTTT
GATTATCCCGCGGCCAGCGCTATCGCCTCGGTAATCCTGGTGGCGTCGCTGCTGCTGCTCTTCGCCATTAACGTGCTGCA
ATCCCGCTTCGGCCGTCGGTTGGGAGGACAATGA

Upstream 100 bases:

>100_bases
TTCGTGTTGAGGATATATTCGGCGGTTGGCATTCGGCTATGGCGACGCACTTCGAGCGCGGCGGCGAACTGGATCAATTA
TTGGCGGCGGGCCATCGCTG

Downstream 100 bases:

>100_bases
TGACAGATTTCTCCGTGTTCAACGGCGCCGCGCGCGCGCCTTTTCCCTGGGGAAAATGGCTATTGATCGGCCTCGGCTCA
GTGGTATCGCTGCTGTTGCT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLVLTTKRVLPGFSLSLGGSVLFVCLILLLPLSALVMQLAQMSWSQYWEVITGPELLAAYKITLICAGVASFFNAVFGML
MAWILTRYQFPGRALLDGLMDLPFALPTAVAGLTLATLFSTTGWYGGCLAQFDIKVSYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAQTLGAGHWQTFRRVILPEVAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILVASLLLLFAINVLQSRFGRRLGGQ

Sequences:

>Translated_277_residues
MLVLTTKRVLPGFSLSLGGSVLFVCLILLLPLSALVMQLAQMSWSQYWEVITGPELLAAYKITLICAGVASFFNAVFGML
MAWILTRYQFPGRALLDGLMDLPFALPTAVAGLTLATLFSTTGWYGGCLAQFDIKVSYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAQTLGAGHWQTFRRVILPEVAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILVASLLLLFAINVLQSRFGRRLGGQ
>Mature_277_residues
MLVLTTKRVLPGFSLSLGGSVLFVCLILLLPLSALVMQLAQMSWSQYWEVITGPELLAAYKITLICAGVASFFNAVFGML
MAWILTRYQFPGRALLDGLMDLPFALPTAVAGLTLATLFSTTGWYGGCLAQFDIKVSYTWLGIAVAMAFTSIPFVVRTVQ
PVLEELGPEYEEAAQTLGAGHWQTFRRVILPEVAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
DYPAASAIASVILVASLLLLFAINVLQSRFGRRLGGQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=277, Percent_Identity=81.9494584837545, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=36.2934362934363, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=34.4978165938865, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787368, Length=216, Percent_Identity=28.7037037037037, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787369, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30041; Mature: 30041

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLTTKRVLPGFSLSLGGSVLFVCLILLLPLSALVMQLAQMSWSQYWEVITGPELLAAY
CEEEECHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KITLICAGVASFFNAVFGMLMAWILTRYQFPGRALLDGLMDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TTGWYGGCLAQFDIKVSYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAQTLGAG
CCCCCCCHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
HWQTFRRVILPEVAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILVASLLLLFAINVLQSRFGRRLGGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLVLTTKRVLPGFSLSLGGSVLFVCLILLLPLSALVMQLAQMSWSQYWEVITGPELLAAY
CEEEECHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KITLICAGVASFFNAVFGMLMAWILTRYQFPGRALLDGLMDLPFALPTAVAGLTLATLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TTGWYGGCLAQFDIKVSYTWLGIAVAMAFTSIPFVVRTVQPVLEELGPEYEEAAQTLGAG
CCCCCCCHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCH
HWQTFRRVILPEVAPALLAGTALSFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFIRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILVASLLLLFAINVLQSRFGRRLGGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]