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The map label for this gene is purF [H]

Identifier: 85059591

GI number: 85059591

Start: 2768736

End: 2770253

Strand: Reverse

Name: purF [H]

Synonym: SG1613

Alternate gene names: 85059591

Gene position: 2770253-2768736 (Counterclockwise)

Preceding gene: 85059592

Following gene: 85059590

Centisome position: 66.41

GC content: 57.11

Gene sequence:

>1518_bases
ATGTGCGGTATTGTCGGAATCGCCGGTTTCATGCCGGTAAACCAGTCTATTTATGATGCATTAACGGTGCTTCAGCACCG
TGGGCAGGATGCGGCAGGCATCGTCACCATCGATGCCTTGAATTGTTTTCGCCTGCGTAAAGCCAATGGACTGGTTAAGG
ATGTTTTCGAGGCCCGGCATATGCAGCGCCTGCAGGGCAATATGGGGGTGGGCCACGTCCGCTACCCGACCGCCGGCAGC
TCTAGCGCCTCCGAGGCGCAGCCGTTTTATGTTAATTCCCCGTTCGGCATCACTCTGGCGCACAACGGTAACCTGACCAA
CGCCCACGAACTGCGTAAAAAGCTGTTTGAAAACGGGCGGCGTCATATCAATACCACCTCCGATTCGGAAATCCTGCTGA
ACGTTTTTGCCGCCGAGCTGGATCGCTTCCCGGATTATCCGCTGGAGGCGGACAACATTTTCGCGGCGGTGGCGGCGACT
CATCAGCAAATCCGCGGCGCCTACGCGTGTGTCGGCATGATTATCGGTCATGGTCTGTTTGCCTTTCGCGATCCCAACGG
CATCCGCCCACTGGTCATCGGCAAGCGCACGCTGGCGGACGGGCGCAATGAATATATTGTGGCCTCCGAAAGCGTGGCGC
TGGATACGCTGGGTTTTGATTTCCTGCGCGATATCGCGCCGGGCGAGGCGGTATACGTCACGGAAAAAGGGCAGCTGTTC
ACCCGGCAGTGCGCTGAGAACCCCAGGCATCATCCTTGTCTGTTCGAGTATGTCTATTTAGCCCGGCCGGACTCGTTCAT
CGACAAAATCTCGGTCTACAGCGCCCGCGTGCGCATGGGCCAAATGCTGGGCGAAAAAATCGCCCGCGAGTGGGAAGATT
TGGATATCGATGTGGTGATCCCGATTCCGGAAACCTCCTGCGATATCGCGCTGGAAATAGCTCGGATACTGAACAAGCCC
TATCGTCAGGGATTTGTAAAAAACCGTTACGTCGGCCGGACCTTCATCATGCCCGGTCAGCAGGTTCGCCGGCAGTCGGT
GCGCCGTAAGCTTAACGCCAACCGCGCCGAGTTCCGCGATAAGAATGTGCTGCTGGTAGACGACTCCATCGCGCGTGGTA
CCACCTCCGAGCAAATCGTCGAGATGGCACGCGAGGCGGGCGCCCGTCACGTATACCTGGCCTCCGCCGCGCCGGAAATC
CGCTTTCCAAACGTTTACGGCATAGATATGCCGAGTGCCTCGGAACTGATTGCCCACGGCCGCGAGGTGGATGAAATACG
GCAGCTCATCGGCGCAGATGCGCTGATATTCCAGGATTTGGCCGATCTGGAACGGGCGGTGCGGGAGGATAATCCTGACG
TTGAGCAATTTGAATCTTCGGTATTCAACGGCATCTACGTGACCCGCGACGTGGATCAGCGCTATCTGGATTATCTGGAG
TCGTTGCGCAACGATGATTCCAAAGTGATGCGCGCCCAGACCGAGGTCGAAAACCTGGAAATGTATAACGAAGGTTGA

Upstream 100 bases:

>100_bases
CAGCGGTATCATCAGATGGTTTTTTGACTATCTGCAAAGCACGTCGAGTTTCTTACCGAGGCAGTAGTTGTCCCGGTAAC
GCTGATGAGGAAATGACAAC

Downstream 100 bases:

>100_bases
TCGGTACCGCGCGGGTGCAGTAGGTTATGTTCCTGACCCGCGCTTTATGGCGGCAGGTCATCTCAATCCGGGCGGTCAAC
CGCGCCGGTTTTTTTCGTCT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGIAGFMPVNQSIYDALTVLQHRGQDAAGIVTIDALNCFRLRKANGLVKDVFEARHMQRLQGNMGVGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHELRKKLFENGRRHINTTSDSEILLNVFAAELDRFPDYPLEADNIFAAVAAT
HQQIRGAYACVGMIIGHGLFAFRDPNGIRPLVIGKRTLADGRNEYIVASESVALDTLGFDFLRDIAPGEAVYVTEKGQLF
TRQCAENPRHHPCLFEYVYLARPDSFIDKISVYSARVRMGQMLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILNKP
YRQGFVKNRYVGRTFIMPGQQVRRQSVRRKLNANRAEFRDKNVLLVDDSIARGTTSEQIVEMAREAGARHVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVDEIRQLIGADALIFQDLADLERAVREDNPDVEQFESSVFNGIYVTRDVDQRYLDYLE
SLRNDDSKVMRAQTEVENLEMYNEG

Sequences:

>Translated_505_residues
MCGIVGIAGFMPVNQSIYDALTVLQHRGQDAAGIVTIDALNCFRLRKANGLVKDVFEARHMQRLQGNMGVGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHELRKKLFENGRRHINTTSDSEILLNVFAAELDRFPDYPLEADNIFAAVAAT
HQQIRGAYACVGMIIGHGLFAFRDPNGIRPLVIGKRTLADGRNEYIVASESVALDTLGFDFLRDIAPGEAVYVTEKGQLF
TRQCAENPRHHPCLFEYVYLARPDSFIDKISVYSARVRMGQMLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILNKP
YRQGFVKNRYVGRTFIMPGQQVRRQSVRRKLNANRAEFRDKNVLLVDDSIARGTTSEQIVEMAREAGARHVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVDEIRQLIGADALIFQDLADLERAVREDNPDVEQFESSVFNGIYVTRDVDQRYLDYLE
SLRNDDSKVMRAQTEVENLEMYNEG
>Mature_505_residues
MCGIVGIAGFMPVNQSIYDALTVLQHRGQDAAGIVTIDALNCFRLRKANGLVKDVFEARHMQRLQGNMGVGHVRYPTAGS
SSASEAQPFYVNSPFGITLAHNGNLTNAHELRKKLFENGRRHINTTSDSEILLNVFAAELDRFPDYPLEADNIFAAVAAT
HQQIRGAYACVGMIIGHGLFAFRDPNGIRPLVIGKRTLADGRNEYIVASESVALDTLGFDFLRDIAPGEAVYVTEKGQLF
TRQCAENPRHHPCLFEYVYLARPDSFIDKISVYSARVRMGQMLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILNKP
YRQGFVKNRYVGRTFIMPGQQVRRQSVRRKLNANRAEFRDKNVLLVDDSIARGTTSEQIVEMAREAGARHVYLASAAPEI
RFPNVYGIDMPSASELIAHGREVDEIRQLIGADALIFQDLADLERAVREDNPDVEQFESSVFNGIYVTRDVDQRYLDYLE
SLRNDDSKVMRAQTEVENLEMYNEG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=35.0951374207188, Blast_Score=268, Evalue=6e-72,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=85.9405940594059, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=255, Percent_Identity=31.3725490196078, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=462, Percent_Identity=33.3333333333333, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6323958, Length=505, Percent_Identity=50.0990099009901, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=32.9875518672199, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=33.1923890063425, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=32.7902240325866, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56679; Mature: 56679

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIAGFMPVNQSIYDALTVLQHRGQDAAGIVTIDALNCFRLRKANGLVKDVFEARH
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHH
MQRLQGNMGVGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHELRKKLFENGR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCC
RHINTTSDSEILLNVFAAELDRFPDYPLEADNIFAAVAATHQQIRGAYACVGMIIGHGLF
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLADGRNEYIVASESVALDTLGFDFLRDIAPGEAVYVTEKGQLF
EEECCCCCCCEEECCHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPRHHPCLFEYVYLARPDSFIDKISVYSARVRMGQMLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALEIARILNKPYRQGFVKNRYVGRTFIMPGQQVRRQSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIARGTTSEQIVEMAREAGARHVYLASAAPEIRFPNVYGIDMPSASELIAHG
CCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQLIGADALIFQDLADLERAVREDNPDVEQFESSVFNGIYVTRDVDQRYLDYLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHH
SLRNDDSKVMRAQTEVENLEMYNEG
HHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MCGIVGIAGFMPVNQSIYDALTVLQHRGQDAAGIVTIDALNCFRLRKANGLVKDVFEARH
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHH
MQRLQGNMGVGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNAHELRKKLFENGR
HHHHHCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCC
RHINTTSDSEILLNVFAAELDRFPDYPLEADNIFAAVAATHQQIRGAYACVGMIIGHGLF
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
AFRDPNGIRPLVIGKRTLADGRNEYIVASESVALDTLGFDFLRDIAPGEAVYVTEKGQLF
EEECCCCCCCEEECCHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
TRQCAENPRHHPCLFEYVYLARPDSFIDKISVYSARVRMGQMLGEKIAREWEDLDIDVVI
HHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
PIPETSCDIALEIARILNKPYRQGFVKNRYVGRTFIMPGQQVRRQSVRRKLNANRAEFRD
ECCCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
KNVLLVDDSIARGTTSEQIVEMAREAGARHVYLASAAPEIRFPNVYGIDMPSASELIAHG
CCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
REVDEIRQLIGADALIFQDLADLERAVREDNPDVEQFESSVFNGIYVTRDVDQRYLDYLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECHHHHHHHHHH
SLRNDDSKVMRAQTEVENLEMYNEG
HHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]