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The map label for this gene is cvpA [H]

Identifier: 85059592

GI number: 85059592

Start: 2770287

End: 2770775

Strand: Reverse

Name: cvpA [H]

Synonym: SG1614

Alternate gene names: 85059592

Gene position: 2770775-2770287 (Counterclockwise)

Preceding gene: 85059593

Following gene: 85059591

Centisome position: 66.43

GC content: 50.31

Gene sequence:

>489_bases
ATGATCTGGGTTGATTACGTCATTATCGGCATCATTGTTTTTTCCGCTTTGGTGAGTCTAATTCGCGGGTTTGTGCGTGA
GGCATTATCTTTAGTGACCTGGGCCTGTGCATTTTTTGTCGCCAGCCATTACTATATTTATCTTGCGATATACTTTACCC
GCTTTGAAGAGCAGATGGTTCGCAGCGGTATTGCGATTGCCCTGCTGTTCGTGGCAACCCTGATCGTCGGGGCAATCGTC
AATTACGTCATTAGTTCGCTAGTCGAGCGGACCGGTTTGTCCGGCACCGACCGGGTATTAGGCGTCTGTTTCGGCGCACT
GCGCGGTGTTTTGATTGTGTCGGCGGCGTTGTTCTTTCTCGACACCTTCACCGGTTTCTCCCACAGTCAGGACTGGCAGC
AGTCCCAACTGATACCGCAATTCAGCGGTATCATCAGATGGTTTTTTGACTATCTGCAAAGCACGTCGAGTTTCTTACCG
AGGCAGTAG

Upstream 100 bases:

>100_bases
ATTTAAACCGGGGGAATAAATCCCCTACGCAAACGTTTTCTTTTTCTGTTAGAATTCGCCGCGAACCAGCTGACCGGGCG
AATGATCAAGGGATGCATGC

Downstream 100 bases:

>100_bases
TTGTCCCGGTAACGCTGATGAGGAAATGACAACATGTGCGGTATTGTCGGAATCGCCGGTTTCATGCCGGTAAACCAGTC
TATTTATGATGCATTAACGG

Product: colicin V production protein

Products: NA

Alternate protein names: Protein dedE; Pur regulon 18 kDa protein [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MIWVDYVIIGIIVFSALVSLIRGFVREALSLVTWACAFFVASHYYIYLAIYFTRFEEQMVRSGIAIALLFVATLIVGAIV
NYVISSLVERTGLSGTDRVLGVCFGALRGVLIVSAALFFLDTFTGFSHSQDWQQSQLIPQFSGIIRWFFDYLQSTSSFLP
RQ

Sequences:

>Translated_162_residues
MIWVDYVIIGIIVFSALVSLIRGFVREALSLVTWACAFFVASHYYIYLAIYFTRFEEQMVRSGIAIALLFVATLIVGAIV
NYVISSLVERTGLSGTDRVLGVCFGALRGVLIVSAALFFLDTFTGFSHSQDWQQSQLIPQFSGIIRWFFDYLQSTSSFLP
RQ
>Mature_162_residues
MIWVDYVIIGIIVFSALVSLIRGFVREALSLVTWACAFFVASHYYIYLAIYFTRFEEQMVRSGIAIALLFVATLIVGAIV
NYVISSLVERTGLSGTDRVLGVCFGALRGVLIVSAALFFLDTFTGFSHSQDWQQSQLIPQFSGIIRWFFDYLQSTSSFLP
RQ

Specific function: Required for colicin V production from plasmid IncFI ColV3-K30 [H]

COG id: COG1286

COG function: function code R; Uncharacterized membrane protein, required for colicin V production

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788652, Length=161, Percent_Identity=78.8819875776398, Blast_Score=262, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003825 [H]

Pfam domain/function: PF02674 Colicin_V [H]

EC number: NA

Molecular weight: Translated: 18234; Mature: 18234

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWVDYVIIGIIVFSALVSLIRGFVREALSLVTWACAFFVASHYYIYLAIYFTRFEEQMV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGIAIALLFVATLIVGAIVNYVISSLVERTGLSGTDRVLGVCFGALRGVLIVSAALFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTFTGFSHSQDWQQSQLIPQFSGIIRWFFDYLQSTSSFLPRQ
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIWVDYVIIGIIVFSALVSLIRGFVREALSLVTWACAFFVASHYYIYLAIYFTRFEEQMV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGIAIALLFVATLIVGAIVNYVISSLVERTGLSGTDRVLGVCFGALRGVLIVSAALFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTFTGFSHSQDWQQSQLIPQFSGIIRWFFDYLQSTSSFLPRQ
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3040734; 2991286; 9205837; 9278503; 2542219 [H]