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The map label for this gene is rsuA [H]

Identifier: 85059548

GI number: 85059548

Start: 2684206

End: 2684913

Strand: Reverse

Name: rsuA [H]

Synonym: SG1570

Alternate gene names: 85059548

Gene position: 2684913-2684206 (Counterclockwise)

Preceding gene: 85059551

Following gene: 85059547

Centisome position: 64.37

GC content: 61.44

Gene sequence:

>708_bases
ATGCGACTGGACAAATTTCTCTCTCAACAGCTGGGCGTCAGCCGGGCCTTGGTGGCGCGCGAGCTGCGCGCCAAACGTGT
CACCGTGGACGGCGAGATTATCAAACAAGGGGCGTTCCAACTGCGGCCCGAACATCAGGTGCGCTTTGACGGCAACCTCC
TGCACCAGATTCTGACCGCGCGCTATTTCATGCTGAATAAGCCCCAAGGCTATGTGTGCTCAACGGACGACCCCGATCAT
CCCACGATCCTGTATTTCATCGACGAACCGGTACCGGACAAGCTTCATGCCGCCGGCCGGTTGGACATTGATACCACGGG
TCTGGTGCTGTTGACCGATGACGGGCAATGGTCGTACCGGCTGACCTCGCCGCGCCATCACTGCGAAAAGGACTATTTAG
TGACGCTGGAGCAGCCGTTGGCTGCGGAGATCACCGCGCAATTCGCCGCCGGCATCCTGCTGCGCGGCGAGAAATTGCCG
ACCCGTCCGGCCCGCCTGGCAACGCTTTCGCCCCTAAAGGTGCGTCTGACCATCAGCGAAGGCCGCTATCACCAGGTAAA
ACGTATGTTCGCCGCCGTCGGTAATCGGGTGGTGGCGCTGCATCGCGAGCGCATCGGCGCCATCGTGCTGGATGCGCATC
TCCAGCCGGGAGAATACCGCGCGCTGACCGCCGGGGAAGTTGCCGGCGCGGGTCTGCTGGTAAATTAA

Upstream 100 bases:

>100_bases
GCGCGATCCGCTGACGTTTCCTTTTCAGCCGTGGCGGTTTCCCTCTGCGGAAGCGCCGACGTTGCCAGGCGCTCCCAAGA
TTACAGGCAAAACAACGTTA

Downstream 100 bases:

>100_bases
GCCAATAATACATCTCATCAGTTTGTTGAAAGGAGTATGGAGAGTGCATCCCGAACGTAAAAAGCATGTCGGTATCGTGG
TAATACTGGGACTGTTGTCC

Product: 16S rRNA pseudouridylate synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEIIKQGAFQLRPEHQVRFDGNLLHQILTARYFMLNKPQGYVCSTDDPDH
PTILYFIDEPVPDKLHAAGRLDIDTTGLVLLTDDGQWSYRLTSPRHHCEKDYLVTLEQPLAAEITAQFAAGILLRGEKLP
TRPARLATLSPLKVRLTISEGRYHQVKRMFAAVGNRVVALHRERIGAIVLDAHLQPGEYRALTAGEVAGAGLLVN

Sequences:

>Translated_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEIIKQGAFQLRPEHQVRFDGNLLHQILTARYFMLNKPQGYVCSTDDPDH
PTILYFIDEPVPDKLHAAGRLDIDTTGLVLLTDDGQWSYRLTSPRHHCEKDYLVTLEQPLAAEITAQFAAGILLRGEKLP
TRPARLATLSPLKVRLTISEGRYHQVKRMFAAVGNRVVALHRERIGAIVLDAHLQPGEYRALTAGEVAGAGLLVN
>Mature_235_residues
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEIIKQGAFQLRPEHQVRFDGNLLHQILTARYFMLNKPQGYVCSTDDPDH
PTILYFIDEPVPDKLHAAGRLDIDTTGLVLLTDDGQWSYRLTSPRHHCEKDYLVTLEQPLAAEITAQFAAGILLRGEKLP
TRPARLATLSPLKVRLTISEGRYHQVKRMFAAVGNRVVALHRERIGAIVLDAHLQPGEYRALTAGEVAGAGLLVN

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=72.0524017467249, Blast_Score=346, Evalue=8e-97,
Organism=Escherichia coli, GI1787524, Length=235, Percent_Identity=31.9148936170213, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=28.82096069869, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=32.967032967033, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26259; Mature: 26259

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEIIKQGAFQLRPEHQVRFDGNLLHQILTA
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHHHHCCCCCCCCCCEEEECHHHHHHHHHH
RYFMLNKPQGYVCSTDDPDHPTILYFIDEPVPDKLHAAGRLDIDTTGLVLLTDDGQWSYR
HHHEEECCCCCEECCCCCCCCEEEEEECCCCCCHHHCCCCCEECCCCEEEEECCCCEEEE
LTSPRHHCEKDYLVTLEQPLAAEITAQFAAGILLRGEKLPTRPARLATLSPLKVRLTISE
ECCCHHHCCCCEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCEEEEEEECC
GRYHQVKRMFAAVGNRVVALHRERIGAIVLDAHLQPGEYRALTAGEVAGAGLLVN
CCHHHHHHHHHHHCCEEEEEEHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEC
>Mature Secondary Structure
MRLDKFLSQQLGVSRALVARELRAKRVTVDGEIIKQGAFQLRPEHQVRFDGNLLHQILTA
CCHHHHHHHHHCHHHHHHHHHHHHHEEEECHHHHHCCCCCCCCCCEEEECHHHHHHHHHH
RYFMLNKPQGYVCSTDDPDHPTILYFIDEPVPDKLHAAGRLDIDTTGLVLLTDDGQWSYR
HHHEEECCCCCEECCCCCCCCEEEEEECCCCCCHHHCCCCCEECCCCEEEEECCCCEEEE
LTSPRHHCEKDYLVTLEQPLAAEITAQFAAGILLRGEKLPTRPARLATLSPLKVRLTISE
ECCCHHHCCCCEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCEEEEEEECC
GRYHQVKRMFAAVGNRVVALHRERIGAIVLDAHLQPGEYRALTAGEVAGAGLLVN
CCHHHHHHHHHHHCCEEEEEEHHHCCEEEEEEECCCCCEEEEECCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]