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The map label for this gene is bcr [H]

Identifier: 85059547

GI number: 85059547

Start: 2682939

End: 2684162

Strand: Reverse

Name: bcr [H]

Synonym: SG1569

Alternate gene names: 85059547

Gene position: 2684162-2682939 (Counterclockwise)

Preceding gene: 85059548

Following gene: 85059546

Centisome position: 64.35

GC content: 57.19

Gene sequence:

>1224_bases
GTGCATCCCGAACGTAAAAAGCATGTCGGTATCGTGGTAATACTGGGACTGTTGTCCATGTTGATGCCACTGGCTATCGA
TATGTACCTGCCCGCGCTACCGATTATCGCGACGGAATACGGTGTGAGCGCCGGCAGCGTGCAGATGACGCTGAGCGCTT
ATGTCCTGGGTTTCGCCATTGGCCAGCTGTTTTACGGGCCGATGGCCGACTGTCTTGGTCGCAAGCCGGTTATCATGTTC
GGGACCTTCTGCTTCGCGCTGGCCGCGGCCGCGTGCGCGCTGTCGCAATCCGTGGAGATGCTGATTTATATGCGCCTGCT
GCACGGTCTGGCCGCCGCCGCCGCAAGCGTGGTGATCAATGCGCTGATGCGCGATATGTTCACTAAAGATGACTTCTCGC
GCATGATGTCTTTCGTGGTGTTGGTGATGACCGTAGCGCCCTTACTGGCGCCGATCATCGGTGGCGGCCTGCTGGTTTTG
TTAAGCTGGCATGCCATATTCTGGACCATCGGCCTGGCGGCGCTGGTCGCGACCGGACTGGTACTGGTGTTTATTCGCGA
AACGCTGCCGCGCGAGCGGCGACAAAAGTTCCGTCTTAGCACCACCATCGGCAATTTCGGTTCGCTGTTTCGTCACAAAC
GCGTCTTTAGCTACATGTTGGCCAGCGGTTTCTCCTTTGCCGGCATGTTTTCGTTTCTCAGCGCCGGGCCGTTTGTCTAT
ATCGACCTCAACGACGTATCGCCGCAGAACTTCGGCTATTATTTTGCGCTCAATGTCGTATTTTTGTTTATCCTGACGTT
GATTAACAGCCGTTTTGTCGCGCGCTTCGCGGCGCAAAGCATGTTTCGGCTCGGTCTGACGATTCAATTTTCCACCTGTC
TATGGATGATAGTGGTGCTGACGCTGCATTTAGGCTTCATTCCGCTGGTGGTGGGTGTCGCGCTGTTTGTGGGATGTGTT
GCCATGGTGACCTCGAACGGGATGGCGGTGATCCTGGAAGATTTTCCGCATATGGCGGGTACAGCCTCGTCGCTGGCGGG
TACGCTGCGTTTCGGCGTCGGCGCGATTATCGGCGCGCTATTGTCGCTGTCGACGTTTAACAGCGCCTGGCCGATGGTGC
TGTCCATGGCCATTTGCAGCAGCGTTGCGCTGCTTTTGTATCTGTATGCCAGCCGTCCGCGCAAGGGCAAAGCCGCGGGC
CGTGCCGGAAGACTCGAGCGCTAG

Upstream 100 bases:

>100_bases
GAATACCGCGCGCTGACCGCCGGGGAAGTTGCCGGCGCGGGTCTGCTGGTAAATTAAGCCAATAATACATCTCATCAGTT
TGTTGAAAGGAGTATGGAGA

Downstream 100 bases:

>100_bases
GCCCGGCTGATTCGCCGCCGCGCCAGACTGATGTATGCGCTAAACCCGGCTGCGCGGCAGGGGCGCCGGGCGGCCTTGGG
GATCATGTTGTGAAATGTCG

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MHPERKKHVGIVVILGLLSMLMPLAIDMYLPALPIIATEYGVSAGSVQMTLSAYVLGFAIGQLFYGPMADCLGRKPVIMF
GTFCFALAAAACALSQSVEMLIYMRLLHGLAAAAASVVINALMRDMFTKDDFSRMMSFVVLVMTVAPLLAPIIGGGLLVL
LSWHAIFWTIGLAALVATGLVLVFIRETLPRERRQKFRLSTTIGNFGSLFRHKRVFSYMLASGFSFAGMFSFLSAGPFVY
IDLNDVSPQNFGYYFALNVVFLFILTLINSRFVARFAAQSMFRLGLTIQFSTCLWMIVVLTLHLGFIPLVVGVALFVGCV
AMVTSNGMAVILEDFPHMAGTASSLAGTLRFGVGAIIGALLSLSTFNSAWPMVLSMAICSSVALLLYLYASRPRKGKAAG
RAGRLER

Sequences:

>Translated_407_residues
MHPERKKHVGIVVILGLLSMLMPLAIDMYLPALPIIATEYGVSAGSVQMTLSAYVLGFAIGQLFYGPMADCLGRKPVIMF
GTFCFALAAAACALSQSVEMLIYMRLLHGLAAAAASVVINALMRDMFTKDDFSRMMSFVVLVMTVAPLLAPIIGGGLLVL
LSWHAIFWTIGLAALVATGLVLVFIRETLPRERRQKFRLSTTIGNFGSLFRHKRVFSYMLASGFSFAGMFSFLSAGPFVY
IDLNDVSPQNFGYYFALNVVFLFILTLINSRFVARFAAQSMFRLGLTIQFSTCLWMIVVLTLHLGFIPLVVGVALFVGCV
AMVTSNGMAVILEDFPHMAGTASSLAGTLRFGVGAIIGALLSLSTFNSAWPMVLSMAICSSVALLLYLYASRPRKGKAAG
RAGRLER
>Mature_407_residues
MHPERKKHVGIVVILGLLSMLMPLAIDMYLPALPIIATEYGVSAGSVQMTLSAYVLGFAIGQLFYGPMADCLGRKPVIMF
GTFCFALAAAACALSQSVEMLIYMRLLHGLAAAAASVVINALMRDMFTKDDFSRMMSFVVLVMTVAPLLAPIIGGGLLVL
LSWHAIFWTIGLAALVATGLVLVFIRETLPRERRQKFRLSTTIGNFGSLFRHKRVFSYMLASGFSFAGMFSFLSAGPFVY
IDLNDVSPQNFGYYFALNVVFLFILTLINSRFVARFAAQSMFRLGLTIQFSTCLWMIVVLTLHLGFIPLVVGVALFVGCV
AMVTSNGMAVILEDFPHMAGTASSLAGTLRFGVGAIIGALLSLSTFNSAWPMVLSMAICSSVALLLYLYASRPRKGKAAG
RAGRLER

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=395, Percent_Identity=67.8481012658228, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI48994889, Length=375, Percent_Identity=28.8, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87082312, Length=354, Percent_Identity=29.3785310734463, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790146, Length=173, Percent_Identity=32.3699421965318, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787065, Length=229, Percent_Identity=23.5807860262009, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790794, Length=254, Percent_Identity=22.4409448818898, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790195, Length=174, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081983, Length=174, Percent_Identity=27.0114942528736, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789207, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081738, Length=188, Percent_Identity=32.4468085106383, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319657, Length=173, Percent_Identity=25.4335260115607, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=185, Percent_Identity=26.4864864864865, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=167, Percent_Identity=25.1497005988024, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322071, Length=201, Percent_Identity=22.3880597014925, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44135; Mature: 44135

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]