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The map label for this gene is pspA [H]

Identifier: 85059480

GI number: 85059480

Start: 2519265

End: 2519618

Strand: Reverse

Name: pspA [H]

Synonym: SG1502

Alternate gene names: 85059480

Gene position: 2519618-2519265 (Counterclockwise)

Preceding gene: 85059486

Following gene: 85059479

Centisome position: 60.41

GC content: 52.26

Gene sequence:

>354_bases
ATGGACATTTTTTCCCGCTTTACCGACATCGTTAACGCCAACATCAATGCACTGCTGGATAAAGCGGAGGATCTGGAAAA
ACTGGTACGCCTGATGATTCAAGAAATGGAAGATACGCTGGTAGAGGTTCGGACGACGTCCGCGCAGGTGCTGGCGGAAA
AGAAACAGTTGTTACGCCGTATCGAGCAAGCCGACCTTCAGCAGGCAGAGTGGCAGAGCAAAGCAGAACTGGCATTGTTG
AAGTATAAAGAAGATTTAGCGCGTTCCGCGCTGATGGAAAAGCAGCGTTTGGTGAAACTTGCCGCGGCGCTGCGCCAGGA
CGTGAGGGTTATCGAAGAGACCCGACGCGGATGA

Upstream 100 bases:

>100_bases
CAATTAGATATTTTTTACCAGAGAGTTGGCGCGAAAAATGTATTAGTCAAACGGTGTGAGTTAGCCAGACTTTTAACCCG
ATCTTAAGAGGATAATCATA

Downstream 100 bases:

>100_bases
AGTATGAGATCGGCGAGTTGGAAAACAAACTGGCCGAAACCCGCGCCCGTCAGCAGGCGCTAACGCTTCGCCATCAGGCG
GCCTCGTCGTCGCGCGATAT

Product: putative phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MDIFSRFTDIVNANINALLDKAEDLEKLVRLMIQEMEDTLVEVRTTSAQVLAEKKQLLRRIEQADLQQAEWQSKAELALL
KYKEDLARSALMEKQRLVKLAAALRQDVRVIEETRRG

Sequences:

>Translated_117_residues
MDIFSRFTDIVNANINALLDKAEDLEKLVRLMIQEMEDTLVEVRTTSAQVLAEKKQLLRRIEQADLQQAEWQSKAELALL
KYKEDLARSALMEKQRLVKLAAALRQDVRVIEETRRG
>Mature_117_residues
MDIFSRFTDIVNANINALLDKAEDLEKLVRLMIQEMEDTLVEVRTTSAQVLAEKKQLLRRIEQADLQQAEWQSKAELALL
KYKEDLARSALMEKQRLVKLAAALRQDVRVIEETRRG

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=114, Percent_Identity=71.0526315789474, Blast_Score=157, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 13568; Mature: 13568

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFSRFTDIVNANINALLDKAEDLEKLVRLMIQEMEDTLVEVRTTSAQVLAEKKQLLRR
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQADLQQAEWQSKAELALLKYKEDLARSALMEKQRLVKLAAALRQDVRVIEETRRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIFSRFTDIVNANINALLDKAEDLEKLVRLMIQEMEDTLVEVRTTSAQVLAEKKQLLRR
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQADLQQAEWQSKAELALLKYKEDLARSALMEKQRLVKLAAALRQDVRVIEETRRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]