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The map label for this gene is manA [H]

Identifier: 85059439

GI number: 85059439

Start: 2446493

End: 2447662

Strand: Reverse

Name: manA [H]

Synonym: SG1461

Alternate gene names: 85059439

Gene position: 2447662-2446493 (Counterclockwise)

Preceding gene: 85059441

Following gene: 85059438

Centisome position: 58.68

GC content: 58.21

Gene sequence:

>1170_bases
ATGGAAAAGATGATAAATACCCTCCAGCATTACTCCTGGGGCAGTAAAGACGCCCTGACTCGGCTTTACGGAATTGCCAA
TCCCGATGGCCGGCCGATGGCGGAATTGTGGATGGGTGCGCACCCTAAGAGCAGCTCGTGCGTTGTTGATGCGCAAGGCG
ATGAGATAACGCTGCGTTCGCTGATTGCGCGCGATCCGGCGGGCATGCTGGGCCGTGCGGTGGCTGACCGCTTCGGCGAA
CTGCCCTTTTTGTTCAAAGTGCTGTGCGCCGCCGAGCCGTTGTCTATCCAGGTCCACCCCAGTCTAGAGGCGGCGAAAAT
CGGTTTTGCGAGGGAAAATCAGGCGGGTATTGCCCCTGATGCGCCCAACCGCAATTACAAAGACGCCAATCACAAGCCGG
AACTGGTGTTTGCCCTGACGCCATTCCAGGCGATGAACGGCTTTCGTACGCTGTCGTCCATTGTCTCACTGCTACAGCCC
GTCGCGGGGGCCCATCCCGCTATCGCCGCCTTACTTGCCCACCCTTATGAGCAACATTTGGCGCAGCTGTTCGGCGATTT
ACTGTCAATGACCGGCGAGCAAAAATCCCGCGCTCTAGATATCCTTACCGCCACGCTGGCCCATAAGCACGGCGAACCCT
GGGATACCGTGCGAATGATTGCCGACGTCTATCCCGAAGACAGCGGCCTGTTCTCACCGCTGCTGTTGAATGTCATTACC
CTGCAGCCTGGTCAGGCGATGTTTTTATTCGCACAAACCCCCCATGCCTACCTCAAAGGCGTGGCCCTGGAGGTGATGGC
CAACTCGGACAATGTAATGCGCGCCGGGCTGACGCCGAAATACATCGACGTGCCGGAGCTGATGGCCAACGTCCAGTTCG
TCGCCAAACCCGAGGATACGCTATTAACCGTGCCCCAAGAGCAAGAGGGTACGCTGTTCTTCCCGATTCCGGTGGACGAT
TTTGCTTTTTCGCTGCATGCGCTAACGCCAGCGCCGCGCGTGCTGGCCCAGGACAGCGCGGCCATTTTATTTTGCGTTGA
CGGGCAGGCGACCCTAGACGGCAACGGGCAACAAATTACGCTGCAGCCGGGTGAGTCGGTCTTTATCGCCGCGCAGGAGT
CACCCATTAAGGTCAGCGGCAGTGGCCGTTTAGCCCGTGTTTACAACTAA

Upstream 100 bases:

>100_bases
GCCAGGGCATTGGTAGTATAGTCCCACATAAACGCCTACCATTATGCGAGCGGTGAGCGCTATTACAATACGAATCAATA
AATTATTAACGGGAACGATC

Downstream 100 bases:

>100_bases
CGCTAACGCAGGCCGGACGAATTGCTTGTCAGTTGCTACTATTAGTTTCATGGCTTGGGCATTATTCCCTCGGTCATGTG
CGCTTTTTGTTGCCTTAGGC

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEKMINTLQHYSWGSKDALTRLYGIANPDGRPMAELWMGAHPKSSSCVVDAQGDEITLRSLIARDPAGMLGRAVADRFGE
LPFLFKVLCAAEPLSIQVHPSLEAAKIGFARENQAGIAPDAPNRNYKDANHKPELVFALTPFQAMNGFRTLSSIVSLLQP
VAGAHPAIAALLAHPYEQHLAQLFGDLLSMTGEQKSRALDILTATLAHKHGEPWDTVRMIADVYPEDSGLFSPLLLNVIT
LQPGQAMFLFAQTPHAYLKGVALEVMANSDNVMRAGLTPKYIDVPELMANVQFVAKPEDTLLTVPQEQEGTLFFPIPVDD
FAFSLHALTPAPRVLAQDSAAILFCVDGQATLDGNGQQITLQPGESVFIAAQESPIKVSGSGRLARVYN

Sequences:

>Translated_389_residues
MEKMINTLQHYSWGSKDALTRLYGIANPDGRPMAELWMGAHPKSSSCVVDAQGDEITLRSLIARDPAGMLGRAVADRFGE
LPFLFKVLCAAEPLSIQVHPSLEAAKIGFARENQAGIAPDAPNRNYKDANHKPELVFALTPFQAMNGFRTLSSIVSLLQP
VAGAHPAIAALLAHPYEQHLAQLFGDLLSMTGEQKSRALDILTATLAHKHGEPWDTVRMIADVYPEDSGLFSPLLLNVIT
LQPGQAMFLFAQTPHAYLKGVALEVMANSDNVMRAGLTPKYIDVPELMANVQFVAKPEDTLLTVPQEQEGTLFFPIPVDD
FAFSLHALTPAPRVLAQDSAAILFCVDGQATLDGNGQQITLQPGESVFIAAQESPIKVSGSGRLARVYN
>Mature_389_residues
MEKMINTLQHYSWGSKDALTRLYGIANPDGRPMAELWMGAHPKSSSCVVDAQGDEITLRSLIARDPAGMLGRAVADRFGE
LPFLFKVLCAAEPLSIQVHPSLEAAKIGFARENQAGIAPDAPNRNYKDANHKPELVFALTPFQAMNGFRTLSSIVSLLQP
VAGAHPAIAALLAHPYEQHLAQLFGDLLSMTGEQKSRALDILTATLAHKHGEPWDTVRMIADVYPEDSGLFSPLLLNVIT
LQPGQAMFLFAQTPHAYLKGVALEVMANSDNVMRAGLTPKYIDVPELMANVQFVAKPEDTLLTVPQEQEGTLFFPIPVDD
FAFSLHALTPAPRVLAQDSAAILFCVDGQATLDGNGQQITLQPGESVFIAAQESPIKVSGSGRLARVYN

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=407, Percent_Identity=35.8722358722359, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1787899, Length=389, Percent_Identity=68.1233933161954, Blast_Score=550, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71997620, Length=401, Percent_Identity=34.1645885286783, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17557650, Length=403, Percent_Identity=27.2952853598015, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=409, Percent_Identity=30.0733496332518, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21356061, Length=393, Percent_Identity=31.5521628498728, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 42117; Mature: 42117

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMINTLQHYSWGSKDALTRLYGIANPDGRPMAELWMGAHPKSSSCVVDAQGDEITLRS
CHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCHHHH
LIARDPAGMLGRAVADRFGELPFLFKVLCAAEPLSIQVHPSLEAAKIGFARENQAGIAPD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCC
APNRNYKDANHKPELVFALTPFQAMNGFRTLSSIVSLLQPVAGAHPAIAALLAHPYEQHL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHH
AQLFGDLLSMTGEQKSRALDILTATLAHKHGEPWDTVRMIADVYPEDSGLFSPLLLNVIT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHEE
LQPGQAMFLFAQTPHAYLKGVALEVMANSDNVMRAGLTPKYIDVPELMANVQFVAKPEDT
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCEEEECCCCC
LLTVPQEQEGTLFFPIPVDDFAFSLHALTPAPRVLAQDSAAILFCVDGQATLDGNGQQIT
EEECCCCCCCEEEEECCHHHHHHEEEECCCCCHHEECCCCEEEEEECCCEEECCCCCEEE
LQPGESVFIAAQESPIKVSGSGRLARVYN
ECCCCEEEEEECCCCEEECCCCCEEEECC
>Mature Secondary Structure
MEKMINTLQHYSWGSKDALTRLYGIANPDGRPMAELWMGAHPKSSSCVVDAQGDEITLRS
CHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCHHHH
LIARDPAGMLGRAVADRFGELPFLFKVLCAAEPLSIQVHPSLEAAKIGFARENQAGIAPD
HHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCC
APNRNYKDANHKPELVFALTPFQAMNGFRTLSSIVSLLQPVAGAHPAIAALLAHPYEQHL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHH
AQLFGDLLSMTGEQKSRALDILTATLAHKHGEPWDTVRMIADVYPEDSGLFSPLLLNVIT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHEE
LQPGQAMFLFAQTPHAYLKGVALEVMANSDNVMRAGLTPKYIDVPELMANVQFVAKPEDT
ECCCCEEEEEECCCHHHHCCEEEEEEECCCCEEECCCCCCCCCHHHHHHCCEEEECCCCC
LLTVPQEQEGTLFFPIPVDDFAFSLHALTPAPRVLAQDSAAILFCVDGQATLDGNGQQIT
EEECCCCCCCEEEEECCHHHHHHEEEECCCCCHHEECCCCEEEEEECCCEEECCCCCEEE
LQPGESVFIAAQESPIKVSGSGRLARVYN
ECCCCEEEEEECCCCEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]