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The map label for this gene is xthA [H]

Identifier: 85059341

GI number: 85059341

Start: 2264533

End: 2265339

Strand: Reverse

Name: xthA [H]

Synonym: SG1363

Alternate gene names: 85059341

Gene position: 2265339-2264533 (Counterclockwise)

Preceding gene: 85059342

Following gene: 85059340

Centisome position: 54.31

GC content: 55.76

Gene sequence:

>807_bases
ATGAAGTTTGTTTCTTTCAATATCAACGGGTTACGCGCCCGGCCGCATCAGCTTGAAGCCATCATCACTACACTACAACC
GGACGTTATCGGTCTGCAGGAAACCAAAGTCCATGACGATATGTTTCCTCTCGAAGAAGTGTCGCGCCACGGTTACCATG
TTTATTACCACGGTCAGAAGGGGCACTACGGCGTGGCGCTGCTCAGCCGCGAGAAACCTCTGGCTATACGTCGCGGTTTC
AGTACCGACGGCGCAGAGGCACAACGCCGAATTATCATGGCGGATTTTCTCACCCCGAAAGGCGCGTTAACGGTGGTCAA
TGGCTACTTTCCCCAAGGCGAAAATCGTGACCATCCGCTTAAATTTCCGGCCAAAGAGCGTTTTTACCGCGATTTACAGG
GCTATGTGGAGCAAAGCCATCACGGCGAGTCCCTGCTGCTGATTATGGGAGATATGAATATCAGCCCCACCGATCTGGAC
ATTGGTATCGGCGAAGAGAGCCGTAAACGCTGGCTTCGTACCGGTAAGTGCTCCTTTCTGCCGGAGGAGCGGGCGTGGAT
GGAGCGCTTGCTGTCATGGGGACTAGTAGACACTTACCGCCAGGCCAACCCGACCGACGATAGTCGCTACTCCTGGTTTG
ATTATCGCTCGCGGGGCTTTGATGAGAACCGTGGCCTGCGCATCGATCTGTTGCTGGCCTCGAAGCCGCTGGCGGCAGTT
GTCCGGGCGGCCGGCATCGATTACACCATCCGCGCAATGGACAAACCGTCCGATCACGCACCGGTGTGGGCGGATTTCGC
TTTGTGA

Upstream 100 bases:

>100_bases
CTCTCTCGACCTTCACCGACCGGCACGACCACTGACGCACGTTTCCATGACTAGCGGTTTCTGCTAACATCAGCCAAAAT
ACTCTCACCTGGCATAAACG

Downstream 100 bases:

>100_bases
GTACGGCGCAACCGCTCAGTGTGGTAGCAGGGCTTATCCTGCGCGACGGCGCCCTGCTGCTGGCCCGGCGCGGCGACAAC
CGCGATCAGCCGGGTTTATG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKFVSFNINGLRARPHQLEAIITTLQPDVIGLQETKVHDDMFPLEEVSRHGYHVYYHGQKGHYGVALLSREKPLAIRRGF
STDGAEAQRRIIMADFLTPKGALTVVNGYFPQGENRDHPLKFPAKERFYRDLQGYVEQSHHGESLLLIMGDMNISPTDLD
IGIGEESRKRWLRTGKCSFLPEERAWMERLLSWGLVDTYRQANPTDDSRYSWFDYRSRGFDENRGLRIDLLLASKPLAAV
VRAAGIDYTIRAMDKPSDHAPVWADFAL

Sequences:

>Translated_268_residues
MKFVSFNINGLRARPHQLEAIITTLQPDVIGLQETKVHDDMFPLEEVSRHGYHVYYHGQKGHYGVALLSREKPLAIRRGF
STDGAEAQRRIIMADFLTPKGALTVVNGYFPQGENRDHPLKFPAKERFYRDLQGYVEQSHHGESLLLIMGDMNISPTDLD
IGIGEESRKRWLRTGKCSFLPEERAWMERLLSWGLVDTYRQANPTDDSRYSWFDYRSRGFDENRGLRIDLLLASKPLAAV
VRAAGIDYTIRAMDKPSDHAPVWADFAL
>Mature_268_residues
MKFVSFNINGLRARPHQLEAIITTLQPDVIGLQETKVHDDMFPLEEVSRHGYHVYYHGQKGHYGVALLSREKPLAIRRGF
STDGAEAQRRIIMADFLTPKGALTVVNGYFPQGENRDHPLKFPAKERFYRDLQGYVEQSHHGESLLLIMGDMNISPTDLD
IGIGEESRKRWLRTGKCSFLPEERAWMERLLSWGLVDTYRQANPTDDSRYSWFDYRSRGFDENRGLRIDLLLASKPLAAV
VRAAGIDYTIRAMDKPSDHAPVWADFAL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=277, Percent_Identity=27.4368231046931, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI18375503, Length=277, Percent_Identity=27.4368231046931, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI18375501, Length=277, Percent_Identity=27.4368231046931, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=74.4360902255639, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=30.8270676691729, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=30.8270676691729, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30707; Mature: 30707

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVSFNINGLRARPHQLEAIITTLQPDVIGLQETKVHDDMFPLEEVSRHGYHVYYHGQK
CEEEEECCCCEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
GHYGVALLSREKPLAIRRGFSTDGAEAQRRIIMADFLTPKGALTVVNGYFPQGENRDHPL
CCEEEEEEECCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCC
KFPAKERFYRDLQGYVEQSHHGESLLLIMGDMNISPTDLDIGIGEESRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCC
PEERAWMERLLSWGLVDTYRQANPTDDSRYSWFDYRSRGFDENRGLRIDLLLASKPLAAV
CHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEEECCCCHHHH
VRAAGIDYTIRAMDKPSDHAPVWADFAL
HHHCCCCEEEEECCCCCCCCCEEEECCC
>Mature Secondary Structure
MKFVSFNINGLRARPHQLEAIITTLQPDVIGLQETKVHDDMFPLEEVSRHGYHVYYHGQK
CEEEEECCCCEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
GHYGVALLSREKPLAIRRGFSTDGAEAQRRIIMADFLTPKGALTVVNGYFPQGENRDHPL
CCEEEEEEECCCCEEEECCCCCCCHHHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCC
KFPAKERFYRDLQGYVEQSHHGESLLLIMGDMNISPTDLDIGIGEESRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECCCHHHHHHHHCCCCCCC
PEERAWMERLLSWGLVDTYRQANPTDDSRYSWFDYRSRGFDENRGLRIDLLLASKPLAAV
CHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEHHHHCCCCCCCCEEEEEEECCCCHHHH
VRAAGIDYTIRAMDKPSDHAPVWADFAL
HHHCCCCEEEEECCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]