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The map label for this gene is hisF

Identifier: 85059102

GI number: 85059102

Start: 1867121

End: 1867897

Strand: Reverse

Name: hisF

Synonym: SG1124

Alternate gene names: 85059102

Gene position: 1867897-1867121 (Counterclockwise)

Preceding gene: 85059103

Following gene: 85059101

Centisome position: 44.78

GC content: 58.04

Gene sequence:

>777_bases
ATGCTGGCAAAACGCATAATTCCCTGTCTGGATGTCCGGCAGGGCCAGGTGGTAAAAGGGGTGCAATTCCGAAATCACGA
AATCATCGGCGGTATTGTGCCGCTGGTGTGCCGCTATGCCGAAGAAGGGGCCGATGAGTTGGTATTCTATGATATCACCG
CCTCCGCCGACGCCCGGGTGGTCGACAAGCGCTGGATAGCCGACGTCGCCGAGGTCATCGACATTCCCTTCTGCGTAGCC
GGCGGTATCCAAAGCGTGGCGCAGGCCGGCGAAATCCTCTCTTACGGCGCCGACAAAATTTCGATTAATTCCCCGGCGCT
GGCGGACCCGACGCTCATCAGCCGCCTGGCGGATCGCTTTGGCGTCCAATGTATTGTCGTCGGTATCGATACCTGGTTCG
ACGCCGCGAGCGGGCGTTATCAGGTCAATCAATATACAGGCGACGAAACCCGCACCCACATCACCCCCTGGCAAACCCTG
GACTGGATAGAAGAAGTTCAGCGGCGCGGCGCGGGGGAGCTCGTATTGAATATGATGAATCAGGACGGCGTGCGTAATGG
CTACGATCTGATGCAATTGCGGCAGGTCCGGGAACGCTGCCACGTTCCGCTTATCGCTTCCGGCGGCGCCGGCACGATGG
CGCATTTTCTCTCGGCGTTTCGCGACGCGCAGGTGGACGGCGCACTGGCGGCGTCGGTGTTTCATAAGCAGATCATCAAT
ATCGGTGAATTAAAGCAGTTCTTGGCGGCAGAGGGAGTGGAAATCAGATTATGTTAA

Upstream 100 bases:

>100_bases
GCCTGGCGGACATCGCCCAGCTTCGCCACAGCGGCGTGCAGGGCGTCATCGTCGGCCGCTCGCTGTTGGAAAACAAATTC
ACCGTGGCGGAGGCGCTGGC

Downstream 100 bases:

>100_bases
CCGAACAACAACGCCAGCAGCTGGATTGGCAAAAAACCGGCGGGATGATGCTGCCCGCTATCGTCCAACACGCCGTCTCC
GGCGAGGTTTTGATGCTGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRQGQVVKGVQFRNHEIIGGIVPLVCRYAEEGADELVFYDITASADARVVDKRWIADVAEVIDIPFCVA
GGIQSVAQAGEILSYGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFDAASGRYQVNQYTGDETRTHITPWQTL
DWIEEVQRRGAGELVLNMMNQDGVRNGYDLMQLRQVRERCHVPLIASGGAGTMAHFLSAFRDAQVDGALAASVFHKQIIN
IGELKQFLAAEGVEIRLC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRQGQVVKGVQFRNHEIIGGIVPLVCRYAEEGADELVFYDITASADARVVDKRWIADVAEVIDIPFCVA
GGIQSVAQAGEILSYGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFDAASGRYQVNQYTGDETRTHITPWQTL
DWIEEVQRRGAGELVLNMMNQDGVRNGYDLMQLRQVRERCHVPLIASGGAGTMAHFLSAFRDAQVDGALAASVFHKQIIN
IGELKQFLAAEGVEIRLC
>Mature_258_residues
MLAKRIIPCLDVRQGQVVKGVQFRNHEIIGGIVPLVCRYAEEGADELVFYDITASADARVVDKRWIADVAEVIDIPFCVA
GGIQSVAQAGEILSYGADKISINSPALADPTLISRLADRFGVQCIVVGIDTWFDAASGRYQVNQYTGDETRTHITPWQTL
DWIEEVQRRGAGELVLNMMNQDGVRNGYDLMQLRQVRERCHVPLIASGGAGTMAHFLSAFRDAQVDGALAASVFHKQIIN
IGELKQFLAAEGVEIRLC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=82.9457364341085, Blast_Score=453, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=29.2452830188679, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SODGM (Q2NTX6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454804.1
- ProteinModelPortal:   Q2NTX6
- SMR:   Q2NTX6
- STRING:   Q2NTX6
- GeneID:   3867552
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1124
- NMPDR:   fig|343509.6.peg.2307
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- PhylomeDB:   Q2NTX6
- ProtClustDB:   PRK02083
- BioCyc:   SGLO343509:SG1124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28330; Mature: 28330

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRQGQVVKGVQFRNHEIIGGIVPLVCRYAEEGADELVFYDITASADARV
CCHHHHCCHHCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
VDKRWIADVAEVIDIPFCVAGGIQSVAQAGEILSYGADKISINSPALADPTLISRLADRF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDAASGRYQVNQYTGDETRTHITPWQTLDWIEEVQRRGAGELVLNMMN
CCEEEEEEECHHHCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLMQLRQVRERCHVPLIASGGAGTMAHFLSAFRDAQVDGALAASVFHKQIIN
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQFLAAEGVEIRLC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRQGQVVKGVQFRNHEIIGGIVPLVCRYAEEGADELVFYDITASADARV
CCHHHHCCHHCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHH
VDKRWIADVAEVIDIPFCVAGGIQSVAQAGEILSYGADKISINSPALADPTLISRLADRF
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDAASGRYQVNQYTGDETRTHITPWQTLDWIEEVQRRGAGELVLNMMN
CCEEEEEEECHHHCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHH
QDGVRNGYDLMQLRQVRERCHVPLIASGGAGTMAHFLSAFRDAQVDGALAASVFHKQIIN
CCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
IGELKQFLAAEGVEIRLC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA