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The map label for this gene is ybiS [H]

Identifier: 85059020

GI number: 85059020

Start: 1730410

End: 1731537

Strand: Reverse

Name: ybiS [H]

Synonym: SG1042

Alternate gene names: 85059020

Gene position: 1731537-1730410 (Counterclockwise)

Preceding gene: 85059025

Following gene: 85059019

Centisome position: 41.51

GC content: 55.85

Gene sequence:

>1128_bases
ATGAAAATGAAAATTCGCGTGTTCTGTAGCATGGTATTGGCAATGGCTGTTTACAGTCAGTCCGCCTTCGCCGTTGTTTA
TCCGCTGCCGGAAGGTAATAGCCGCCTGGTGGGGGAAAATCTGCAAATCACCGTTCCCGAAGGGAACAAGCTTCCCCTAG
AACATTTTGCCGCTCAGTTCCAAATGGGGCTGAGCAATATGCTTGAAGCCAATCCGGGCGTGGATGTCTATCTACCGGCC
GCCGGCACCAAGATGATCATTCCGCAACAGTTGATTTTGCCCGACACGCCGCATGAGGGTATTGTGATTAACAGTGCCGA
AATGCGGTTGTATTATTATCCGAAAGGCACCAAGACGGTCATCGTGTTGCCCATTGGTATTGGCGAGCTGGGCAAAGACA
CCCCCTCCAATTGGGTGACCTCGGTGCAGCGTAAAAAAGATGGTCCTACCTGGACACCGACCAAAGCCATACATGCAGAA
TATGCCGAGCGCGGTGAAACGCTGCCGGAAGTGTTTCCGGCAGGCCCGGATAACCCGATGGGGTTGTATGCGCTCTATGT
CGGCCGCCTGTATGCGATTCATGGCACCAACGCTAACTTCGGTATCGGCCTGCGCGTGAGCCACGGCTGCGTGCGTTTGC
GCGCCGACGATATCAAATATCTGTTTGATCATGTTCCGGTCGGCACCCGCGTTCAATTCATCAATGAACCGGTAAAGGCC
ACGATCGAACCGGATGGCTCACGTTATCTGGAAGTGCACAACCCGCTCTCTGTCACCGAAGAGCAGTTTAACTCCAACGA
GCCGGTACCGATTAATCTGCCCGTCAACGTCAGCCATGTACTGGTGGACCCCAGCGTAAACCAAAGTACCGTCGACCAAA
CCCTGCAGCGTCGTTCCGGCATGCCGGAAAATATCACCGCGCAGGGCGACAGCAGCCAAACCGCAACGGTACAGGTACAG
CCGGTTGATGAGCGTACCATTGCCGAAGGTCCCAAAACCGAGCAGGCGCAGCAGGGCAACGCCGCCCCGGTCACCCTGCA
AACCAGCGTGCCTGAACAAAACGGCGCTACGGTCCCAGTGGACAACACCGCGCAGGCGCAAAATACCGGCGGGTGCCGCG
CCAATTGA

Upstream 100 bases:

>100_bases
TTCCACGCCGGGGATTGAGCTCCTATAATGCACATTCGGCACTCTATACCGGGTAAACACACTCATTGACAGCCTGTAAG
GCGATAACATCTGACAATTT

Downstream 100 bases:

>100_bases
CCAGGCCACGCCTGCTGCCGGCGGCGACGTTCAAACCCCGCAATAATTCCGCGGTCTCGGCTTGTGCCGATCGGACCACC
CATGACGCAGGTTGCCTCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKMKIRVFCSMVLAMAVYSQSAFAVVYPLPEGNSRLVGENLQITVPEGNKLPLEHFAAQFQMGLSNMLEANPGVDVYLPA
AGTKMIIPQQLILPDTPHEGIVINSAEMRLYYYPKGTKTVIVLPIGIGELGKDTPSNWVTSVQRKKDGPTWTPTKAIHAE
YAERGETLPEVFPAGPDNPMGLYALYVGRLYAIHGTNANFGIGLRVSHGCVRLRADDIKYLFDHVPVGTRVQFINEPVKA
TIEPDGSRYLEVHNPLSVTEEQFNSNEPVPINLPVNVSHVLVDPSVNQSTVDQTLQRRSGMPENITAQGDSSQTATVQVQ
PVDERTIAEGPKTEQAQQGNAAPVTLQTSVPEQNGATVPVDNTAQAQNTGGCRAN

Sequences:

>Translated_375_residues
MKMKIRVFCSMVLAMAVYSQSAFAVVYPLPEGNSRLVGENLQITVPEGNKLPLEHFAAQFQMGLSNMLEANPGVDVYLPA
AGTKMIIPQQLILPDTPHEGIVINSAEMRLYYYPKGTKTVIVLPIGIGELGKDTPSNWVTSVQRKKDGPTWTPTKAIHAE
YAERGETLPEVFPAGPDNPMGLYALYVGRLYAIHGTNANFGIGLRVSHGCVRLRADDIKYLFDHVPVGTRVQFINEPVKA
TIEPDGSRYLEVHNPLSVTEEQFNSNEPVPINLPVNVSHVLVDPSVNQSTVDQTLQRRSGMPENITAQGDSSQTATVQVQ
PVDERTIAEGPKTEQAQQGNAAPVTLQTSVPEQNGATVPVDNTAQAQNTGGCRAN
>Mature_375_residues
MKMKIRVFCSMVLAMAVYSQSAFAVVYPLPEGNSRLVGENLQITVPEGNKLPLEHFAAQFQMGLSNMLEANPGVDVYLPA
AGTKMIIPQQLILPDTPHEGIVINSAEMRLYYYPKGTKTVIVLPIGIGELGKDTPSNWVTSVQRKKDGPTWTPTKAIHAE
YAERGETLPEVFPAGPDNPMGLYALYVGRLYAIHGTNANFGIGLRVSHGCVRLRADDIKYLFDHVPVGTRVQFINEPVKA
TIEPDGSRYLEVHNPLSVTEEQFNSNEPVPINLPVNVSHVLVDPSVNQSTVDQTLQRRSGMPENITAQGDSSQTATVQVQ
PVDERTIAEGPKTEQAQQGNAAPVTLQTSVPEQNGATVPVDNTAQAQNTGGCRAN

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=302, Percent_Identity=70.1986754966888, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788299, Length=304, Percent_Identity=59.8684210526316, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787356, Length=292, Percent_Identity=48.2876712328767, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787968, Length=309, Percent_Identity=46.6019417475728, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 40734; Mature: 40734

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKIRVFCSMVLAMAVYSQSAFAVVYPLPEGNSRLVGENLQITVPEGNKLPLEHFAAQF
CCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCEEEECCCCCCCCHHHHHHHH
QMGLSNMLEANPGVDVYLPAAGTKMIIPQQLILPDTPHEGIVINSAEMRLYYYPKGTKTV
HHHHHHHHHCCCCCEEEECCCCCEEECCCCEECCCCCCCCEEEECCEEEEEEECCCCCEE
IVLPIGIGELGKDTPSNWVTSVQRKKDGPTWTPTKAIHAEYAERGETLPEVFPAGPDNPM
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCC
GLYALYVGRLYAIHGTNANFGIGLRVSHGCVRLRADDIKYLFDHVPVGTRVQFINEPVKA
HHHEEEEEEEEEEECCCCCEEEEEEEECCEEEEEHHHHHHHHHHCCCCCEEEEECCCEEE
TIEPDGSRYLEVHNPLSVTEEQFNSNEPVPINLPVNVSHVLVDPSVNQSTVDQTLQRRSG
EECCCCCEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
MPENITAQGDSSQTATVQVQPVDERTIAEGPKTEQAQQGNAAPVTLQTSVPEQNGATVPV
CCCCEEECCCCCCEEEEEEEECCCCHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCEECC
DNTAQAQNTGGCRAN
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKMKIRVFCSMVLAMAVYSQSAFAVVYPLPEGNSRLVGENLQITVPEGNKLPLEHFAAQF
CCEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCEEEECCCCCCCCHHHHHHHH
QMGLSNMLEANPGVDVYLPAAGTKMIIPQQLILPDTPHEGIVINSAEMRLYYYPKGTKTV
HHHHHHHHHCCCCCEEEECCCCCEEECCCCEECCCCCCCCEEEECCEEEEEEECCCCCEE
IVLPIGIGELGKDTPSNWVTSVQRKKDGPTWTPTKAIHAEYAERGETLPEVFPAGPDNPM
EEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCC
GLYALYVGRLYAIHGTNANFGIGLRVSHGCVRLRADDIKYLFDHVPVGTRVQFINEPVKA
HHHEEEEEEEEEEECCCCCEEEEEEEECCEEEEEHHHHHHHHHHCCCCCEEEEECCCEEE
TIEPDGSRYLEVHNPLSVTEEQFNSNEPVPINLPVNVSHVLVDPSVNQSTVDQTLQRRSG
EECCCCCEEEEECCCCHHHHHHHCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHCC
MPENITAQGDSSQTATVQVQPVDERTIAEGPKTEQAQQGNAAPVTLQTSVPEQNGATVPV
CCCCEEECCCCCCEEEEEEEECCCCHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCEECC
DNTAQAQNTGGCRAN
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]