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The map label for this gene is htrB [H]

Identifier: 85059025

GI number: 85059025

Start: 1746424

End: 1747347

Strand: Reverse

Name: htrB [H]

Synonym: SG1047

Alternate gene names: 85059025

Gene position: 1747347-1746424 (Counterclockwise)

Preceding gene: 85059027

Following gene: 85059020

Centisome position: 41.89

GC content: 58.55

Gene sequence:

>924_bases
ATGACCACACTTCCTGTTTTTAATCGTTCTTTGCTGCATCCGCGTTATTGGCTAACCTGGCTGGGTATCGGGGCTCTCTA
TTTGCTGGTGCTGCTGCCTTATCCGCTGCTCTACGCGCTGGGTACCCGTCTGGGACGGCTTGCGATGCGCTTCATGCGCC
ACAGAGAGGCGATCGCGCGCCGTAATCTGCAATTGTGTTTTCCGGCCATGCCGGAAGCGGAGCGTGAAGCGCTGTTGCGG
CAGAATTTTGAATCGGTCGGCATGGGGCTTATCGAAACCAGCATCGCCTGGTTCTGGCCTGATTGGCGTATCAAGCGCTG
GATCTCCGTCAGCGGCCTGGAGCATATCGCCGGCGCGCGCGCGGAAAATAAAGGGGTGCTGCTTATCGGCATGCATTTTC
TCACTCTTGAACTGGGGGCGCGGATTTTTGGCATGTATAACCCCGGTATCGGCGTTTATCGCCCCAACGACAACCCGCTG
CTGGACTGGCTGCAAACCTGGGGGCGTATGCGCTCCAACAAATCCATGCTGGACCGGTCGGATATTAAAGGCATGATTCG
CGCTTTGAAAAACGGCGATATCATCTGGTACGCGCCGGATCATGACTATGGCCCCAAAAGCAGCGTATTCGCGCCGCTGT
TCGCCGTTCCCCAGGCCGCGACCACCGCCGGCACCTATTTGCTGGCCAAAACGGCCAAACCGGCGGTGATCCCGTTCATG
CCGCGCCGTTTGCCTCAGGGGCGCGGGTATGAGCTGTTGATTCTGCCCGATGAGCGCGACATGCCGCTGGATAGCGATAT
TGCCGCCGCCAGCTATATGAACAAGGTGGTGGAGCAGGCGATACTGCAGGCGCCGGATCAATACATGTGGCTGCATCGGC
GCTTTAAAACCCGCCCGCCCGGCGAACCTTCGTTGTACCGCTGA

Upstream 100 bases:

>100_bases
GCTTTATTCCACCGCAGCAAAAAAAATGCCAGAATATTGCAAGGTATTACGGCACGGCTGAAGGTACGGGCTTACCCGTC
ACATTCACACTTGGGACGCT

Downstream 100 bases:

>100_bases
TTTTTGCCGCGTCGGCCTCAGCCGGCATGGGGATTCCGGTGAAATTGCCCCTGCCGCCCTTTCGGCGCATACTGTGTCCC
TGACTTAATCTCCCCCTGCG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTLPVFNRSLLHPRYWLTWLGIGALYLLVLLPYPLLYALGTRLGRLAMRFMRHREAIARRNLQLCFPAMPEAEREALLR
QNFESVGMGLIETSIAWFWPDWRIKRWISVSGLEHIAGARAENKGVLLIGMHFLTLELGARIFGMYNPGIGVYRPNDNPL
LDWLQTWGRMRSNKSMLDRSDIKGMIRALKNGDIIWYAPDHDYGPKSSVFAPLFAVPQAATTAGTYLLAKTAKPAVIPFM
PRRLPQGRGYELLILPDERDMPLDSDIAAASYMNKVVEQAILQAPDQYMWLHRRFKTRPPGEPSLYR

Sequences:

>Translated_307_residues
MTTLPVFNRSLLHPRYWLTWLGIGALYLLVLLPYPLLYALGTRLGRLAMRFMRHREAIARRNLQLCFPAMPEAEREALLR
QNFESVGMGLIETSIAWFWPDWRIKRWISVSGLEHIAGARAENKGVLLIGMHFLTLELGARIFGMYNPGIGVYRPNDNPL
LDWLQTWGRMRSNKSMLDRSDIKGMIRALKNGDIIWYAPDHDYGPKSSVFAPLFAVPQAATTAGTYLLAKTAKPAVIPFM
PRRLPQGRGYELLILPDERDMPLDSDIAAASYMNKVVEQAILQAPDQYMWLHRRFKTRPPGEPSLYR
>Mature_306_residues
TTLPVFNRSLLHPRYWLTWLGIGALYLLVLLPYPLLYALGTRLGRLAMRFMRHREAIARRNLQLCFPAMPEAEREALLRQ
NFESVGMGLIETSIAWFWPDWRIKRWISVSGLEHIAGARAENKGVLLIGMHFLTLELGARIFGMYNPGIGVYRPNDNPLL
DWLQTWGRMRSNKSMLDRSDIKGMIRALKNGDIIWYAPDHDYGPKSSVFAPLFAVPQAATTAGTYLLAKTAKPAVIPFMP
RRLPQGRGYELLILPDERDMPLDSDIAAASYMNKVVEQAILQAPDQYMWLHRRFKTRPPGEPSLYR

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=66.3398692810458, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082094, Length=300, Percent_Identity=51.6666666666667, Blast_Score=351, Evalue=3e-98,
Organism=Escherichia coli, GI1788161, Length=309, Percent_Identity=31.0679611650485, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35181; Mature: 35050

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLPVFNRSLLHPRYWLTWLGIGALYLLVLLPYPLLYALGTRLGRLAMRFMRHREAIAR
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQLCFPAMPEAEREALLRQNFESVGMGLIETSIAWFWPDWRIKRWISVSGLEHIAGAR
CCCEEECCCCCHHHHHHHHHHCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AENKGVLLIGMHFLTLELGARIFGMYNPGIGVYRPNDNPLLDWLQTWGRMRSNKSMLDRS
CCCCCEEEEHHHHHHHHHHHHHEEEECCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DIKGMIRALKNGDIIWYAPDHDYGPKSSVFAPLFAVPQAATTAGTYLLAKTAKPAVIPFM
HHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHHHCCEEEEECCCCCCCCCC
PRRLPQGRGYELLILPDERDMPLDSDIAAASYMNKVVEQAILQAPDQYMWLHRRFKTRPP
HHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GEPSLYR
CCCCCCC
>Mature Secondary Structure 
TTLPVFNRSLLHPRYWLTWLGIGALYLLVLLPYPLLYALGTRLGRLAMRFMRHREAIAR
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQLCFPAMPEAEREALLRQNFESVGMGLIETSIAWFWPDWRIKRWISVSGLEHIAGAR
CCCEEECCCCCHHHHHHHHHHCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
AENKGVLLIGMHFLTLELGARIFGMYNPGIGVYRPNDNPLLDWLQTWGRMRSNKSMLDRS
CCCCCEEEEHHHHHHHHHHHHHEEEECCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DIKGMIRALKNGDIIWYAPDHDYGPKSSVFAPLFAVPQAATTAGTYLLAKTAKPAVIPFM
HHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHHHCCEEEEECCCCCCCCCC
PRRLPQGRGYELLILPDERDMPLDSDIAAASYMNKVVEQAILQAPDQYMWLHRRFKTRPP
HHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GEPSLYR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]