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The map label for this gene is pflA

Identifier: 85058965

GI number: 85058965

Start: 1623918

End: 1624658

Strand: Reverse

Name: pflA

Synonym: SG0987

Alternate gene names: 85058965

Gene position: 1624658-1623918 (Counterclockwise)

Preceding gene: 85058966

Following gene: 85058955

Centisome position: 38.95

GC content: 53.17

Gene sequence:

>741_bases
ATGTCTGTTACTGGTCGTATCCATTCCTTTGAATCCTGCGGAACCGTAGATGGCCCAGGCATTCGGTTTATCATTTTTTT
CCAGGGTTGCCTGATGCGCTGTTTGTATTGCCATAATCGCGATACCTGGGATACCCACGGCGGACGGGAAATTACCGTTG
AGGAGATCATGCGTGAGGTGATCTCCTACCGCCACTTTATGAACGCGTCCGGCGGCGGCGTCACCGCGTCCGGCGGCGAG
GCGATTTTGCAGGCAGAGTTTGTACGAGACTGGTTCCGCGCCTGCCATGCCGAAGGCATCAACACCTGCGTGGATACCAA
CGGCTTTGTTCGCCGCTATGATCCGGTGATTGACGAATTGCTGGAGGTCACCGATTTGGTCATGCTGGATATCAAAGAGA
TGAACGATGATATCCATCAGAATCTGGTCGGGGTTTCCAATCATCGTACACTGGATTTCGCACGTTACCTGGCCAAGATT
AATAAGCGGACCTGGTTGCGCTATGTGGTGGTGCCGGGTTGGTCGGATGACGATAAATCCGTGCACATGCTGGGCGAGTT
TACCCAGCATATGAGCAATATCGAGAAAATCGAGCTGCTGCCTTATCATGAGCTGGGTAAGCACAAATGGATCGCCATGG
GCGAGGAGTACCGCCTTGAGGGCGTTAAACCGCCCACCACCGCGAGCATGGATCACATCCGTGACATTTTGGCCGGCTAC
GGTCATAAGGTTATGTACTGA

Upstream 100 bases:

>100_bases
CGGCGCACCCAATGGAACAGCGCGTTTTGTCTGACGGCTATACTTACCGTAAGCAGTCTTTCAAACTTATTATTCTATTA
TCAATGGAGAACACCCTGCC

Downstream 100 bases:

>100_bases
CCAGGCGGGCTCTTCCGCGTCTCCTGCGCTCGGGTTCCGCTGCCGGCAGCGCAGCGACGATGTAAGCCCAGCGGCAACGT
AGCGTGGCGCAATGTTATCG

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVTGRIHSFESCGTVDGPGIRFIIFFQGCLMRCLYCHNRDTWDTHGGREITVEEIMREVISYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACHAEGINTCVDTNGFVRRYDPVIDELLEVTDLVMLDIKEMNDDIHQNLVGVSNHRTLDFARYLAKI
NKRTWLRYVVVPGWSDDDKSVHMLGEFTQHMSNIEKIELLPYHELGKHKWIAMGEEYRLEGVKPPTTASMDHIRDILAGY
GHKVMY

Sequences:

>Translated_246_residues
MSVTGRIHSFESCGTVDGPGIRFIIFFQGCLMRCLYCHNRDTWDTHGGREITVEEIMREVISYRHFMNASGGGVTASGGE
AILQAEFVRDWFRACHAEGINTCVDTNGFVRRYDPVIDELLEVTDLVMLDIKEMNDDIHQNLVGVSNHRTLDFARYLAKI
NKRTWLRYVVVPGWSDDDKSVHMLGEFTQHMSNIEKIELLPYHELGKHKWIAMGEEYRLEGVKPPTTASMDHIRDILAGY
GHKVMY
>Mature_245_residues
SVTGRIHSFESCGTVDGPGIRFIIFFQGCLMRCLYCHNRDTWDTHGGREITVEEIMREVISYRHFMNASGGGVTASGGEA
ILQAEFVRDWFRACHAEGINTCVDTNGFVRRYDPVIDELLEVTDLVMLDIKEMNDDIHQNLVGVSNHRTLDFARYLAKIN
KRTWLRYVVVPGWSDDDKSVHMLGEFTQHMSNIEKIELLPYHELGKHKWIAMGEEYRLEGVKPPTTASMDHIRDILAGYG
HKVMY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=81.3008130081301, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790389, Length=273, Percent_Identity=26.3736263736264, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI226510931, Length=171, Percent_Identity=32.7485380116959, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28201; Mature: 28069

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTGRIHSFESCGTVDGPGIRFIIFFQGCLMRCLYCHNRDTWDTHGGREITVEEIMREV
CCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
ISYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHAEGINTCVDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHH
LEVTDLVMLDIKEMNDDIHQNLVGVSNHRTLDFARYLAKINKRTWLRYVVVPGWSDDDKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCH
VHMLGEFTQHMSNIEKIELLPYHELGKHKWIAMGEEYRLEGVKPPTTASMDHIRDILAGY
HHHHHHHHHHHCCHHHEEECCHHHHCCCCEEEECCHHEECCCCCCCCCCHHHHHHHHHHC
GHKVMY
CCCCCC
>Mature Secondary Structure 
SVTGRIHSFESCGTVDGPGIRFIIFFQGCLMRCLYCHNRDTWDTHGGREITVEEIMREV
CCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHH
ISYRHFMNASGGGVTASGGEAILQAEFVRDWFRACHAEGINTCVDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHH
LEVTDLVMLDIKEMNDDIHQNLVGVSNHRTLDFARYLAKINKRTWLRYVVVPGWSDDDKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCH
VHMLGEFTQHMSNIEKIELLPYHELGKHKWIAMGEEYRLEGVKPPTTASMDHIRDILAGY
HHHHHHHHHHHCCHHHEEECCHHHHCCCCEEEECCHHEECCCCCCCCCCHHHHHHHHHHC
GHKVMY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]