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The map label for this gene is amsJ [H]

Identifier: 85058955

GI number: 85058955

Start: 1608707

End: 1609828

Strand: Reverse

Name: amsJ [H]

Synonym: SG0977

Alternate gene names: 85058955

Gene position: 1609828-1608707 (Counterclockwise)

Preceding gene: 85058965

Following gene: 85058954

Centisome position: 38.59

GC content: 44.39

Gene sequence:

>1122_bases
ATGAAATTACTTTTGGTTGGTAATCACACCTGCAGCAATAGGGGAGATAGTGCCATTCTGTGCGGTATCGTACAATCGTT
AGAAGAGCTGGCACCCGGTATTCAAATAGACATCGTTAGCCGCTATGCGCAAAGCTCCCGCTACCTCATAGGAAGGGATA
TCAGAAAAGACACTCTCAGCGCGGCCAGGCGCAGCGGCAATGGTGGCTTGGTGCATAAAGTCATTAAAAAGATCGCCAAC
AGATTTACCCATAAAATAATCATATCGCATATCGAGAACAAGGGGCTTTACAAAAGTTTCAAAATCCCCCCCGTTTATCA
AAACTTCATCAATGAAATCAAAAAGTACGATGCCCTTATCAAGGTGGGCGGATCTTTTTTTGTTGATCTCTACGGGTCCC
ACCAGTTTGATCATGCGTTATGCGACTTTTTAGCTAATAAACCCATATTCATGGTCGGACATAGCGTGAACCCCTTCCAA
AAGCAGAGCTTCAACGATGTCGCCCACTTTGTGTTCAAAAAAGTCGATGCACTCATTTTGTGCGAAAGCGTTAGCCTTGA
CGTGATGAAAAAGAGCCAGATACCCACTAATAAGGTCATCGCGGGTATCGACACGGCATGGCTGGTGAAACTCGTATTGA
GCCAAAGTCGTTCTGCTTATGCTCTTGAATATTGGCGGGGGGTGATCGAGGCTTCCGATAGCATTGCGATGACATTTCTC
AAATTAGCGCCTTTCGACAAATGTCTCGGCATTACTCAGCAGGAGTACATGACCGCTTTTGCTAATTTAATCAATGAAAT
CACCCGTTCGGGAAAACAGGTTATCGCTTTTTCAACCTGTACGGGGATTGATAGCTATCACAATGATGATCGTATGATTG
CCCTTCATATGAAGGACATAGTGGAAAGCCCCGATAGGTTCCATGTCATTATGGACAAGTTTAATGATCTGGAGCTGGGT
GAGTTGCTCTCTCATTGCAAACTGACTATCGGCACCCGGCTTCATTCGGCCATTATTTCAATAAATTTTGGCACGCCGGC
TATTGCTATTAATTATGAGCATAAATCTCTGGGTATTATGCAGCAGTTAGGGTTGCCCGAATTGGCTGTTGATATCAAAT
AA

Upstream 100 bases:

>100_bases
AAACCCTTTTATATTGTCATATTCTTTTACACTTTTGTTTTATTGGTGGGCTGTAAAAAAATTTTGCGCTTGATTTCTAT
AATTTTCTAATGAGGTAGAA

Downstream 100 bases:

>100_bases
TTGATTAATGGGCAACTTATTGTAAAATATCATAATGCAATAGAAAACATTTCCTCACTGGAACTGCAGGTTAAAAATGC
AGTAGCACAGGAGCAGGACA

Product: amylovoran biosynthesis protein AmsJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKLLLVGNHTCSNRGDSAILCGIVQSLEELAPGIQIDIVSRYAQSSRYLIGRDIRKDTLSAARRSGNGGLVHKVIKKIAN
RFTHKIIISHIENKGLYKSFKIPPVYQNFINEIKKYDALIKVGGSFFVDLYGSHQFDHALCDFLANKPIFMVGHSVNPFQ
KQSFNDVAHFVFKKVDALILCESVSLDVMKKSQIPTNKVIAGIDTAWLVKLVLSQSRSAYALEYWRGVIEASDSIAMTFL
KLAPFDKCLGITQQEYMTAFANLINEITRSGKQVIAFSTCTGIDSYHNDDRMIALHMKDIVESPDRFHVIMDKFNDLELG
ELLSHCKLTIGTRLHSAIISINFGTPAIAINYEHKSLGIMQQLGLPELAVDIK

Sequences:

>Translated_373_residues
MKLLLVGNHTCSNRGDSAILCGIVQSLEELAPGIQIDIVSRYAQSSRYLIGRDIRKDTLSAARRSGNGGLVHKVIKKIAN
RFTHKIIISHIENKGLYKSFKIPPVYQNFINEIKKYDALIKVGGSFFVDLYGSHQFDHALCDFLANKPIFMVGHSVNPFQ
KQSFNDVAHFVFKKVDALILCESVSLDVMKKSQIPTNKVIAGIDTAWLVKLVLSQSRSAYALEYWRGVIEASDSIAMTFL
KLAPFDKCLGITQQEYMTAFANLINEITRSGKQVIAFSTCTGIDSYHNDDRMIALHMKDIVESPDRFHVIMDKFNDLELG
ELLSHCKLTIGTRLHSAIISINFGTPAIAINYEHKSLGIMQQLGLPELAVDIK
>Mature_373_residues
MKLLLVGNHTCSNRGDSAILCGIVQSLEELAPGIQIDIVSRYAQSSRYLIGRDIRKDTLSAARRSGNGGLVHKVIKKIAN
RFTHKIIISHIENKGLYKSFKIPPVYQNFINEIKKYDALIKVGGSFFVDLYGSHQFDHALCDFLANKPIFMVGHSVNPFQ
KQSFNDVAHFVFKKVDALILCESVSLDVMKKSQIPTNKVIAGIDTAWLVKLVLSQSRSAYALEYWRGVIEASDSIAMTFL
KLAPFDKCLGITQQEYMTAFANLINEITRSGKQVIAFSTCTGIDSYHNDDRMIALHMKDIVESPDRFHVIMDKFNDLELG
ELLSHCKLTIGTRLHSAIISINFGTPAIAINYEHKSLGIMQQLGLPELAVDIK

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor [H]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=375, Percent_Identity=53.0666666666667, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 41693; Mature: 41693

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLLVGNHTCSNRGDSAILCGIVQSLEELAPGIQIDIVSRYAQSSRYLIGRDIRKDTLS
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEECCHHHHHHHH
AARRSGNGGLVHKVIKKIANRFTHKIIISHIENKGLYKSFKIPPVYQNFINEIKKYDALI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHH
KVGGSFFVDLYGSHQFDHALCDFLANKPIFMVGHSVNPFQKQSFNDVAHFVFKKVDALIL
HCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEE
CESVSLDVMKKSQIPTNKVIAGIDTAWLVKLVLSQSRSAYALEYWRGVIEASDSIAMTFL
ECCCCHHHHHHCCCCHHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
KLAPFDKCLGITQQEYMTAFANLINEITRSGKQVIAFSTCTGIDSYHNDDRMIALHMKDI
HHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEHHH
VESPDRFHVIMDKFNDLELGELLSHCKLTIGTRLHSAIISINFGTPAIAINYEHKSLGIM
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECHHHHHHH
QQLGLPELAVDIK
HHCCCCCEEEECC
>Mature Secondary Structure
MKLLLVGNHTCSNRGDSAILCGIVQSLEELAPGIQIDIVSRYAQSSRYLIGRDIRKDTLS
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCEEECCHHHHHHHH
AARRSGNGGLVHKVIKKIANRFTHKIIISHIENKGLYKSFKIPPVYQNFINEIKKYDALI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHH
KVGGSFFVDLYGSHQFDHALCDFLANKPIFMVGHSVNPFQKQSFNDVAHFVFKKVDALIL
HCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEE
CESVSLDVMKKSQIPTNKVIAGIDTAWLVKLVLSQSRSAYALEYWRGVIEASDSIAMTFL
ECCCCHHHHHHCCCCHHHEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
KLAPFDKCLGITQQEYMTAFANLINEITRSGKQVIAFSTCTGIDSYHNDDRMIALHMKDI
HHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEEEHHH
VESPDRFHVIMDKFNDLELGELLSHCKLTIGTRLHSAIISINFGTPAIAINYEHKSLGIM
HCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECHHHHHHH
QQLGLPELAVDIK
HHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]