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The map label for this gene is miaB

Identifier: 85058789

GI number: 85058789

Start: 1367519

End: 1368943

Strand: Reverse

Name: miaB

Synonym: SG0811

Alternate gene names: 85058789

Gene position: 1368943-1367519 (Counterclockwise)

Preceding gene: 85058791

Following gene: 85058788

Centisome position: 32.82

GC content: 56.07

Gene sequence:

>1425_bases
ATGACCAAAAAACTCCATATCAAAACCTGGGGCTGTCAGATGAATGAATACGATTCATCCAAGATGGCCGACTTACTGGA
TAGCACTCACGGCTATCAGTTGACGGACAACCCCGAAGAGGCGGATGTCCTGCTGCTTAACACCTGCTCCATCCGGGAAA
AAGCGCAGGAAAAGGTGTTCCATCAGCTGGGGCGCTGGCACATGCTTAAGGAAGCCCGACCGGAATTGATCATCGGCGTC
GGCGGCTGCGTGGCGTCGCAGGAAGGCGCCCATATCCGTGAGCGGGCCAATTACGTGGATATCATTTTCGGGCCTCAGAC
GCTGCACCGCCTGCCGGAAATGATTAATCATGTCAATGGCACCCGCAGCCCGGTGGTGGATATCAGCTTTCCGGAAATCG
AAAAATTCGATCGTCTGCCGGAACCGCGCGCCGAAGGGCCGACCGCCTTTGTGTCTATCATGGAAGGCTGCAACAAATAC
TGCAGTTTTTGCGTCGTACCCTATACCCGCGGCGAAGAAGTCAGCCGCCCGTGCGACGATATCCTGTTCGAGATAGCCCA
GCTTGCGGATCAGGGTGTGCGTGAAGTCAACCTGCTGGGACAGAACGTCAACGCCTATCGCGGCGCCAGCTATGACGGCG
GCATCTGCACCTTCGCCGAACTGCTGCGGCTGGTGGCGGCCATCGACGGTATCGACCGTATCCGCTATATCACCAGCCAC
CCTATTGAGTTTACCGACGATATTATCGACGTTTACCGCGATACGCCGGAACTGGTCAGCTTCGTGCACCTGCCGGTGCA
AAGCGGCTCCGATCGCATCCTGACCATGATGAAACGCGCCCACACCGCGCTGGAATATAAATCGATCATCCGCCGGCTGC
GCAAGGCGCGCCCGGATATCCAAATCAGCTCCGACTTCATCATCGGCTTTCCGGGCGAAACCCAGCAAGACTTTGAGCAA
ACGATGCAGCTTATCGCCGACGTGAATTTCGACATGAGCTTCAGCTTCATTTACTCCGCGCGTCCCGGCACGCCTGCCGC
TGATATGGTGGATAATGTCAGCGAAGAAGAGAAAAAACAGCGGCTCCATATCTTGCAGGAACGTATCACCCAACAGGCCA
TGCAGTACAGCCGCCGCATGAAGGGTAAAGTGCAACGTATTCTGGTGGAAGGCACATCGCGCAAAAATGTGATGGAGCTG
TCCGGCCGTACCGAAAACAATCGGGTGGTCAATTTTGAAGGCGCGCCGTCCATGATTGGCAAATTCGTCGATGTGGAGAT
CGTCGACGTGTATCCCAATTCCCTGCGCGGCGTCTTGTTGCGCACCGAAGATCAGATGACATTGCGCAGCCATGAAAGCA
CGGCGTCGGTCATTGCCCGCACCCGTAAAGAGAACGAACTGGGCGTGGGCATGTACCAGCCGTGA

Upstream 100 bases:

>100_bases
CTCGCGCGCTAGACTGGTCACAACACCGGCAAAGGATTACAATACGCGCCCTGCGCAAGCCTACGGGCTTTTTAATACTG
CATCGAGTACTGGCAAGCTG

Downstream 100 bases:

>100_bases
CCCCCCTGCCCGCCAGCTTTCCGGAGCGGCTTGCCGCTCCGCCGCCATGCGGCTCACCTGCTTGCATTGCACTAATTGTG
CCCCAATATACATATAATGG

Product: putative tRNA-thiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWHMLKEARPELIIGV
GGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNGTRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CSFCVVPYTRGEEVSRPCDDILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSH
PIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDIQISSDFIIGFPGETQQDFEQ
TMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQRLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMEL
SGRTENNRVVNFEGAPSMIGKFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWHMLKEARPELIIGV
GGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNGTRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CSFCVVPYTRGEEVSRPCDDILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSH
PIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDIQISSDFIIGFPGETQQDFEQ
TMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQRLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMEL
SGRTENNRVVNFEGAPSMIGKFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVFHQLGRWHMLKEARPELIIGVG
GCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNGTRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
SFCVVPYTRGEEVSRPCDDILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSHP
IEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDIQISSDFIIGFPGETQQDFEQT
MQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQRLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMELS
GRTENNRVVNFEGAPSMIGKFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=36.5904365904366, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=36.5904365904366, Blast_Score=287, Evalue=2e-77,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=28.6343612334802, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=85.2320675105485, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=390, Percent_Identity=28.974358974359, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.6133909287257, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=257, Percent_Identity=31.1284046692607, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=35.3348729792148, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922432, Length=485, Percent_Identity=28.8659793814433, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SODGM (Q2NUT9)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454491.1
- ProteinModelPortal:   Q2NUT9
- SMR:   Q2NUT9
- STRING:   Q2NUT9
- GeneID:   3868020
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0811
- NMPDR:   fig|343509.6.peg.1739
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2NUT9
- BioCyc:   SGLO343509:SG0811-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53799; Mature: 53668

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWHMLKEARPELIIGVGGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNG
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHCCC
TRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
ILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSH
HHHHHHHHHHCCHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
PIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIIGFPGETQQDFEQTMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCHHHHHHH
RLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMELSGRTENNRVVNFEGAPSMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCEEEEECCCHHHHH
KFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP
HHHCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWHMLKEARPELIIGVGGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNG
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHCCC
TRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
ILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSH
HHHHHHHHHHCCHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
PIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIIGFPGETQQDFEQTMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCHHHHHHH
RLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMELSGRTENNRVVNFEGAPSMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCEEEEECCCHHHHH
KFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP
HHHCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA