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The map label for this gene is arrD [H]

Identifier: 85058706

GI number: 85058706

Start: 1215147

End: 1215644

Strand: Reverse

Name: arrD [H]

Synonym: SG0728

Alternate gene names: 85058706

Gene position: 1215644-1215147 (Counterclockwise)

Preceding gene: 85058707

Following gene: 85058705

Centisome position: 29.14

GC content: 47.19

Gene sequence:

>498_bases
ATGTCTCCCGAACTTCGTAAACGATTGATAACAGTGACGTCAGGTGGCGTATTAGCGCTGGCTGCCGTCTTGGTGCAGTG
GCACGAAGGTAAGCGCTATAAGCCCTATCGTGATGGCGGCGGGGTGCTCACTGTCTGTCACGGTCATACCGGCAAGGACG
TTACGCCAGGGGAAATTTATAACGAAGAAGAATGTAACGCGTTGATGAAAAAGGATTTGCAGGTAGCACGTGCTACTGTT
GAACGTTATGTCACTGTTCAGCTTACCGATTTGCAGAAAGCCGCTTTGACTTCATTTGTTTACAATATCGGCAGTGGGGC
ATTTGCCAATTCAACGCTACTTAAAAAGTTGAACGCCGGAGATATTCAAGGCGCTTGTGACCAGATGCGCCGATGGAAAT
ACGATGAAGGAAAGGTATCAAACGGGCTGATTAACCGCAGAGAGGTAGAACGGGAAATTTGTTTAAATCCGAATGCACTA
ATCAATCCAACTCAATGA

Upstream 100 bases:

>100_bases
GGGATTTTCTCTACTTCCAGGGTTGGGATAACGGGCAGTATGAAACGTTCACACCCGACATGTACCAATACTGGGCTGAA
CTACCGATCGGATTACTATA

Downstream 100 bases:

>100_bases
GCCTTGGTTGTCGTTCAAGGCTTTTCTATACGCTCCGCTGTGTAAAGAATCACATCAAACCTGAACCTTTTGAAATGAGC
CTTTGAGGAAGTCGGTTAGT

Product: phage lysozyme lysis protein

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSPELRKRLITVTSGGVLALAAVLVQWHEGKRYKPYRDGGGVLTVCHGHTGKDVTPGEIYNEEECNALMKKDLQVARATV
ERYVTVQLTDLQKAALTSFVYNIGSGAFANSTLLKKLNAGDIQGACDQMRRWKYDEGKVSNGLINRREVEREICLNPNAL
INPTQ

Sequences:

>Translated_165_residues
MSPELRKRLITVTSGGVLALAAVLVQWHEGKRYKPYRDGGGVLTVCHGHTGKDVTPGEIYNEEECNALMKKDLQVARATV
ERYVTVQLTDLQKAALTSFVYNIGSGAFANSTLLKKLNAGDIQGACDQMRRWKYDEGKVSNGLINRREVEREICLNPNAL
INPTQ
>Mature_164_residues
SPELRKRLITVTSGGVLALAAVLVQWHEGKRYKPYRDGGGVLTVCHGHTGKDVTPGEIYNEEECNALMKKDLQVARATVE
RYVTVQLTDLQKAALTSFVYNIGSGAFANSTLLKKLNAGDIQGACDQMRRWKYDEGKVSNGLINRREVEREICLNPNALI
NPTQ

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=161, Percent_Identity=49.6894409937888, Blast_Score=154, Evalue=2e-39,
Organism=Escherichia coli, GI1787836, Length=114, Percent_Identity=43.859649122807, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 18277; Mature: 18146

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPELRKRLITVTSGGVLALAAVLVQWHEGKRYKPYRDGGGVLTVCHGHTGKDVTPGEIY
CCHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
NEEECNALMKKDLQVARATVERYVTVQLTDLQKAALTSFVYNIGSGAFANSTLLKKLNAG
CHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
DIQGACDQMRRWKYDEGKVSNGLINRREVEREICLNPNALINPTQ
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SPELRKRLITVTSGGVLALAAVLVQWHEGKRYKPYRDGGGVLTVCHGHTGKDVTPGEIY
CHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
NEEECNALMKKDLQVARATVERYVTVQLTDLQKAALTSFVYNIGSGAFANSTLLKKLNAG
CHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
DIQGACDQMRRWKYDEGKVSNGLINRREVEREICLNPNALINPTQ
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]