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The map label for this gene is rnhA

Identifier: 85058569

GI number: 85058569

Start: 998648

End: 999139

Strand: Reverse

Name: rnhA

Synonym: SG0591

Alternate gene names: 85058569

Gene position: 999139-998648 (Counterclockwise)

Preceding gene: 85058572

Following gene: 85058567

Centisome position: 23.95

GC content: 58.94

Gene sequence:

>492_bases
ATGCGTAAACAGGTTGCAATTTTCACCGACGGTTCGTGCCTGGGCAATCCCGGTCCCGGCGGCTATGGCGCCATACTGCG
CTATAAGCAGCACGAAAAAACCTTCAGCGCCGGTTATCGCCTGACCACCAATAATCGCATGGAGCTGATGGCGGCGATTG
TGGCATTGGAGGCGCTGACGGATGCCTGCGAGGTGGTGCTTAGTACCGACAGCCAGTACGTCCGCCAGGGGATTACCCAA
TGGATACACAACTGGAAAAAGCGGGGCTGGAAAACCGCCTACAAGAAACCCGTGAAAAACGTCGACTTATGGCAGCGGCT
GGACGCGGCCATTCAGCCTCATACCCTGCGCTGGGATTGGGTAAAGGGCCACTCGGGCCACCCGGAAAACGAACGCTGCG
ATGAACTGGCTCGCACCGCCGCCTGCCATCCGGCATTGGAGGACATCGGTTATCGAGTGGAAGCGCAAACGAGCGGCGGC
CGGGCGGATTAA

Upstream 100 bases:

>100_bases
GTGGTTTCGGTATCCAGAACAATCTGTCGCGTAATGTCAGTGCTCATAATGCTCGTTTATGTCAGACTTGGGCTTTTAAC
ATGGAGCAAGTTTACCAGAG

Downstream 100 bases:

>100_bases
ATCGGGTTCACTTTGTTTATGAGACATGCCGTTGCCGGCAGCTGCGGGTGGTGGCGTTAACCGGCCGGCTTAATCCCCGG
GAGGGCGTCGAGTTCTTCAG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MRKQVAIFTDGSCLGNPGPGGYGAILRYKQHEKTFSAGYRLTTNNRMELMAAIVALEALTDACEVVLSTDSQYVRQGITQ
WIHNWKKRGWKTAYKKPVKNVDLWQRLDAAIQPHTLRWDWVKGHSGHPENERCDELARTAACHPALEDIGYRVEAQTSGG
RAD

Sequences:

>Translated_163_residues
MRKQVAIFTDGSCLGNPGPGGYGAILRYKQHEKTFSAGYRLTTNNRMELMAAIVALEALTDACEVVLSTDSQYVRQGITQ
WIHNWKKRGWKTAYKKPVKNVDLWQRLDAAIQPHTLRWDWVKGHSGHPENERCDELARTAACHPALEDIGYRVEAQTSGG
RAD
>Mature_163_residues
MRKQVAIFTDGSCLGNPGPGGYGAILRYKQHEKTFSAGYRLTTNNRMELMAAIVALEALTDACEVVLSTDSQYVRQGITQ
WIHNWKKRGWKTAYKKPVKNVDLWQRLDAAIQPHTLRWDWVKGHSGHPENERCDELARTAACHPALEDIGYRVEAQTSGG
RAD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=37.3239436619718, Blast_Score=80, Evalue=8e-16,
Organism=Escherichia coli, GI1786408, Length=155, Percent_Identity=82.5806451612903, Blast_Score=273, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=35.7142857142857, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505482, Length=131, Percent_Identity=35.8778625954198, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=37.5, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=37.5, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SODGM (Q2NVF9)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454271.1
- ProteinModelPortal:   Q2NVF9
- SMR:   Q2NVF9
- STRING:   Q2NVF9
- GeneID:   3866659
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0591
- NMPDR:   fig|343509.6.peg.1277
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2NVF9
- ProtClustDB:   PRK00203
- BioCyc:   SGLO343509:SG0591-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18368; Mature: 18368

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQVAIFTDGSCLGNPGPGGYGAILRYKQHEKTFSAGYRLTTNNRMELMAAIVALEALT
CCCEEEEEECCCCCCCCCCCCHHHHEEHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHH
DACEVVLSTDSQYVRQGITQWIHNWKKRGWKTAYKKPVKNVDLWQRLDAAIQPHTLRWDW
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEE
VKGHSGHPENERCDELARTAACHPALEDIGYRVEAQTSGGRAD
ECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MRKQVAIFTDGSCLGNPGPGGYGAILRYKQHEKTFSAGYRLTTNNRMELMAAIVALEALT
CCCEEEEEECCCCCCCCCCCCHHHHEEHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHH
DACEVVLSTDSQYVRQGITQWIHNWKKRGWKTAYKKPVKNVDLWQRLDAAIQPHTLRWDW
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCEEEEE
VKGHSGHPENERCDELARTAACHPALEDIGYRVEAQTSGGRAD
ECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA