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The map label for this gene is gloB

Identifier: 85058567

GI number: 85058567

Start: 997104

End: 997856

Strand: Reverse

Name: gloB

Synonym: SG0589

Alternate gene names: 85058567

Gene position: 997856-997104 (Counterclockwise)

Preceding gene: 85058569

Following gene: 85058566

Centisome position: 23.92

GC content: 49.27

Gene sequence:

>753_bases
ATGAATCTTATCAGCATTCCCGCATTGGCGGATAATTACATTTGGCTTTTACACAATGACGACAGGCGCTGCCTGGTGGT
CGATCCGGGCGATGCGACGCCGGTGTTGCAAGCGCTGGCCGACCACCGGCTGACCCCTGTCGCCGTTTTATTGACTCATC
ATCACCAGGACCATGTAGGCGGCGTTAGCGAGCTGCTGCAGCATTTTCCCGTGCCAGTCTATGGCCCAGAAGAAACCCGT
TCCAAAGGCGCAACGCAGATAGTCAACGAAGGCGATAGCCTGACATTACTGGACCACACCTTCAGCATAATGGCATTACC
CGGTCATACACTCGGACATATCGGATTTTATAGCGCGCCCTGGCTTTTTTGCGGCGACACGGTTTTTTCCGCAGGTAGCG
GCCGGCTGTTTGAGGGCACACCCAGGCAAATGTATGAGTCTTTTCAGAAGGTTAACCAGCTTTCGTCCGCTACCCTTATT
TGTGCCGCACATGAGTATACTTTAAGCAATTTAAACTTTGCTGCCGCCCTATTACCCCAAGATAGCGTTATCACTGATTA
TCAACGTAAAATTAAAGAGTTACGGCTGAAAAACCAGCCCAGCATGCCGACAAAACTGCATTTAGAGCGACAAATTAATC
TTTTCTTACGTTGCCATGACGTTAATTTACAAAACAAATTAAACGTTCACCCCGCTCCCAGCGAGGAATGGCGTGTTTTT
GCGGCATTACGCGAGAAGAAGGATCATTTCTAA

Upstream 100 bases:

>100_bases
ATCGTCCCATGAGACAGGCGCCTTAATCGTTTTGTTACTTTGCGCTGGTTTCATGTTTGTCTATCTTCTAAGTGCCATAG
AAAAAATAACGAGGTTAACA

Downstream 100 bases:

>100_bases
AGGTTTTATTTGTAATGTCCCAGCCTTCAAAGTACCATTGCCCGTCTTTTAAGCGACTATTGACATACAGATGAAGACTC
ATGCGATCTTATTCGCCTCG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNLISIPALADNYIWLLHNDDRRCLVVDPGDATPVLQALADHRLTPVAVLLTHHHQDHVGGVSELLQHFPVPVYGPEETR
SKGATQIVNEGDSLTLLDHTFSIMALPGHTLGHIGFYSAPWLFCGDTVFSAGSGRLFEGTPRQMYESFQKVNQLSSATLI
CAAHEYTLSNLNFAAALLPQDSVITDYQRKIKELRLKNQPSMPTKLHLERQINLFLRCHDVNLQNKLNVHPAPSEEWRVF
AALREKKDHF

Sequences:

>Translated_250_residues
MNLISIPALADNYIWLLHNDDRRCLVVDPGDATPVLQALADHRLTPVAVLLTHHHQDHVGGVSELLQHFPVPVYGPEETR
SKGATQIVNEGDSLTLLDHTFSIMALPGHTLGHIGFYSAPWLFCGDTVFSAGSGRLFEGTPRQMYESFQKVNQLSSATLI
CAAHEYTLSNLNFAAALLPQDSVITDYQRKIKELRLKNQPSMPTKLHLERQINLFLRCHDVNLQNKLNVHPAPSEEWRVF
AALREKKDHF
>Mature_250_residues
MNLISIPALADNYIWLLHNDDRRCLVVDPGDATPVLQALADHRLTPVAVLLTHHHQDHVGGVSELLQHFPVPVYGPEETR
SKGATQIVNEGDSLTLLDHTFSIMALPGHTLGHIGFYSAPWLFCGDTVFSAGSGRLFEGTPRQMYESFQKVNQLSSATLI
CAAHEYTLSNLNFAAALLPQDSVITDYQRKIKELRLKNQPSMPTKLHLERQINLFLRCHDVNLQNKLNVHPAPSEEWRVF
AALREKKDHF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=31.1538461538462, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=31.1538461538462, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI21703352, Length=245, Percent_Identity=33.0612244897959, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI116642887, Length=243, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI14150041, Length=226, Percent_Identity=32.7433628318584, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI46361987, Length=188, Percent_Identity=34.0425531914894, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=56.9721115537849, Blast_Score=290, Evalue=6e-80,
Organism=Escherichia coli, GI1787158, Length=156, Percent_Identity=35.2564102564103, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536925, Length=257, Percent_Identity=28.4046692607004, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320478, Length=190, Percent_Identity=30.5263157894737, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324614, Length=191, Percent_Identity=30.3664921465969, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=33.7164750957854, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=33.7164750957854, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=33.7164750957854, Blast_Score=143, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_SODGM (Q2NVG1)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454269.1
- ProteinModelPortal:   Q2NVG1
- SMR:   Q2NVG1
- STRING:   Q2NVG1
- GeneID:   3866657
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0589
- NMPDR:   fig|343509.6.peg.1275
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q2NVG1
- BioCyc:   SGLO343509:SG0589-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28100; Mature: 28100

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLISIPALADNYIWLLHNDDRRCLVVDPGDATPVLQALADHRLTPVAVLLTHHHQDHVG
CCEEECCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCHHCC
GVSELLQHFPVPVYGPEETRSKGATQIVNEGDSLTLLDHTFSIMALPGHTLGHIGFYSAP
CHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCCCEEEEECCEEEEEECCCCHHCCCCCCCC
WLFCGDTVFSAGSGRLFEGTPRQMYESFQKVNQLSSATLICAAHEYTLSNLNFAAALLPQ
EEEECCHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEECEEECCCCCEEEEECCC
DSVITDYQRKIKELRLKNQPSMPTKLHLERQINLFLRCHDVNLQNKLNVHPAPSEEWRVF
CCHHHHHHHHHHHHHHCCCCCCCCEEEHEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHH
AALREKKDHF
HHHHHHCCCC
>Mature Secondary Structure
MNLISIPALADNYIWLLHNDDRRCLVVDPGDATPVLQALADHRLTPVAVLLTHHHQDHVG
CCEEECCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCHHCC
GVSELLQHFPVPVYGPEETRSKGATQIVNEGDSLTLLDHTFSIMALPGHTLGHIGFYSAP
CHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCCCEEEEECCEEEEEECCCCHHCCCCCCCC
WLFCGDTVFSAGSGRLFEGTPRQMYESFQKVNQLSSATLICAAHEYTLSNLNFAAALLPQ
EEEECCHHHCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEECEEECCCCCEEEEECCC
DSVITDYQRKIKELRLKNQPSMPTKLHLERQINLFLRCHDVNLQNKLNVHPAPSEEWRVF
CCHHHHHHHHHHHHHHCCCCCCCCEEEHEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHH
AALREKKDHF
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA