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The map label for this gene is mltD

Identifier: 85058566

GI number: 85058566

Start: 995663

End: 997033

Strand: Reverse

Name: mltD

Synonym: SG0588

Alternate gene names: 85058566

Gene position: 997033-995663 (Counterclockwise)

Preceding gene: 85058567

Following gene: 85058564

Centisome position: 23.9

GC content: 55.0

Gene sequence:

>1371_bases
ATGAAGACTCATGCGATCTTATTCGCCTCGGTCTTGCTGGTGGGGTGTCAGGCGTCCAGGCAGGACACGCCACCCCACGA
ACAGCATGCACAGAGTGTTTCTTCGGCAGGTCAAGGTGAAGCAGGAAAGTACACAGATGACCGAGCGGGCACCGCGCAAT
GGCAAGATATTAACACTAGCCTGCCGCAAGGCGATTTGTGGGACTTCATTGGCGACGAGCTGAAGATGCATATACCGGAT
AACGCCCGGGTCCGCGAGCAACAAAAGCGTTATTTGAAACAGAAGAGCTATCTCCACGATGTAACATTACGGGCAGAGCC
GTACGTGTACTGGATTGTCGAGCAGATTAAGCAACGTAAGATGCCGATGGAATTGGTACTGCTACCCATAGTGGAGAGCG
CTTTTGACCCTAAGGCAACATCCGCCTCTAACGCGGCTGGCCTTTGGCAGATTGTTCCCAGGACAGGCAAGAATTATGGC
TTGAAGCAAAATCAGTGGTATGATGGCCGCCGTGATGTGGTGGCGTCTACCACCGTTGCGCTGGATATGATGCAGCGGCT
TAACCGTATGTTCGACGGCGACTGGCTATTGACTATCGCTGCCTATAACAGCGGTGAAGGCCGTATTATGAAGGCCATTA
AGCGTAATAAGGCCAGGGGCCGCCCTACCGACTTCTGGTCGTTGGCGCTGCCTCGCGAAACCACCATTTATGTTCCGAAG
ATGCTTGCGCTTAGCAACCTCATTAAGAATAGCAAACAATTTGGCATCGCGTTGCCTAAATCGAATGCACAGCGCGCACT
GGCGCGGGTGGAAGTCGGCCAGCAGATCGGCCTGACGCAGGCGGCGGAAATGTCGGGCCTGTCGCTGACCAAGTTGAAAA
GCTTCAACACTGGCTATAAGCGCAACGTGACGGCGCCCAATGGACCGCATTACATAATGCTACCCAAGGAGCATGCCTCC
CAGCTGCGTACCTCGCTGGCGGAAGGCGGCATTGCGGTGGTACAGTCCACGCAGCTTGCCGATAACCTCGGCGGCAGCTA
TGAGGTCCGCGCCGGAGATACGTTATCCGGCATCGCACAACGGCTTAACGTTAAAACCGCTGAACTGCATAAGTGGAATA
ATCTGCGCAGCGCGCGTAGCCTGAAGGTGGGCCAGACGCTGAAAGTTGGCAGCGGCACGATGCAGATTGCCAACAACAAC
AGCATTACCTATCAGGTGCGCAAGGGGGATTCCCTGGAGAGCATAGCCCGCCGGCACGGCGTCGATATCAAAGACGTAAT
GCGCTGGAACGATGCGTTGACTAATGCGAACAACCTGCAGCCGGGGCTTAAGCTCACGCTGTTCGTTAACGGCAAGTCAA
CGGATTCCTGA

Upstream 100 bases:

>100_bases
GCATTACGCGAGAAGAAGGATCATTTCTAAAGGTTTTATTTGTAATGTCCCAGCCTTCAAAGTACCATTGCCCGTCTTTT
AAGCGACTATTGACATACAG

Downstream 100 bases:

>100_bases
GGCAGCGCTGATCGCCTCGTTTTTTCGGCGATGTGAGACAGCACGACGCGATGCCTGGGCAGATTCATGCCACCGTTAGC
ATTATAAAAAGCATACGCTA

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKTHAILFASVLLVGCQASRQDTPPHEQHAQSVSSAGQGEAGKYTDDRAGTAQWQDINTSLPQGDLWDFIGDELKMHIPD
NARVREQQKRYLKQKSYLHDVTLRAEPYVYWIVEQIKQRKMPMELVLLPIVESAFDPKATSASNAAGLWQIVPRTGKNYG
LKQNQWYDGRRDVVASTTVALDMMQRLNRMFDGDWLLTIAAYNSGEGRIMKAIKRNKARGRPTDFWSLALPRETTIYVPK
MLALSNLIKNSKQFGIALPKSNAQRALARVEVGQQIGLTQAAEMSGLSLTKLKSFNTGYKRNVTAPNGPHYIMLPKEHAS
QLRTSLAEGGIAVVQSTQLADNLGGSYEVRAGDTLSGIAQRLNVKTAELHKWNNLRSARSLKVGQTLKVGSGTMQIANNN
SITYQVRKGDSLESIARRHGVDIKDVMRWNDALTNANNLQPGLKLTLFVNGKSTDS

Sequences:

>Translated_456_residues
MKTHAILFASVLLVGCQASRQDTPPHEQHAQSVSSAGQGEAGKYTDDRAGTAQWQDINTSLPQGDLWDFIGDELKMHIPD
NARVREQQKRYLKQKSYLHDVTLRAEPYVYWIVEQIKQRKMPMELVLLPIVESAFDPKATSASNAAGLWQIVPRTGKNYG
LKQNQWYDGRRDVVASTTVALDMMQRLNRMFDGDWLLTIAAYNSGEGRIMKAIKRNKARGRPTDFWSLALPRETTIYVPK
MLALSNLIKNSKQFGIALPKSNAQRALARVEVGQQIGLTQAAEMSGLSLTKLKSFNTGYKRNVTAPNGPHYIMLPKEHAS
QLRTSLAEGGIAVVQSTQLADNLGGSYEVRAGDTLSGIAQRLNVKTAELHKWNNLRSARSLKVGQTLKVGSGTMQIANNN
SITYQVRKGDSLESIARRHGVDIKDVMRWNDALTNANNLQPGLKLTLFVNGKSTDS
>Mature_456_residues
MKTHAILFASVLLVGCQASRQDTPPHEQHAQSVSSAGQGEAGKYTDDRAGTAQWQDINTSLPQGDLWDFIGDELKMHIPD
NARVREQQKRYLKQKSYLHDVTLRAEPYVYWIVEQIKQRKMPMELVLLPIVESAFDPKATSASNAAGLWQIVPRTGKNYG
LKQNQWYDGRRDVVASTTVALDMMQRLNRMFDGDWLLTIAAYNSGEGRIMKAIKRNKARGRPTDFWSLALPRETTIYVPK
MLALSNLIKNSKQFGIALPKSNAQRALARVEVGQQIGLTQAAEMSGLSLTKLKSFNTGYKRNVTAPNGPHYIMLPKEHAS
QLRTSLAEGGIAVVQSTQLADNLGGSYEVRAGDTLSGIAQRLNVKTAELHKWNNLRSARSLKVGQTLKVGSGTMQIANNN
SITYQVRKGDSLESIARRHGVDIKDVMRWNDALTNANNLQPGLKLTLFVNGKSTDS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=456, Percent_Identity=64.0350877192982, Blast_Score=588, Evalue=1e-169,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50628; Mature: 50628

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHAILFASVLLVGCQASRQDTPPHEQHAQSVSSAGQGEAGKYTDDRAGTAQWQDINTS
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC
LPQGDLWDFIGDELKMHIPDNARVREQQKRYLKQKSYLHDVTLRAEPYVYWIVEQIKQRK
CCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHC
MPMELVLLPIVESAFDPKATSASNAAGLWQIVPRTGKNYGLKQNQWYDGRRDVVASTTVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LDMMQRLNRMFDGDWLLTIAAYNSGEGRIMKAIKRNKARGRPTDFWSLALPRETTIYVPK
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHH
MLALSNLIKNSKQFGIALPKSNAQRALARVEVGQQIGLTQAAEMSGLSLTKLKSFNTGYK
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCCCC
RNVTAPNGPHYIMLPKEHASQLRTSLAEGGIAVVQSTQLADNLGGSYEVRAGDTLSGIAQ
CCCCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCEEEECCCHHHHHHH
RLNVKTAELHKWNNLRSARSLKVGQTLKVGSGTMQIANNNSITYQVRKGDSLESIARRHG
HHCCCHHHHHHHHCHHHHHCCCCCCEEEECCCEEEEECCCEEEEEEECCCCHHHHHHHHC
VDIKDVMRWNDALTNANNLQPGLKLTLFVNGKSTDS
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTHAILFASVLLVGCQASRQDTPPHEQHAQSVSSAGQGEAGKYTDDRAGTAQWQDINTS
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC
LPQGDLWDFIGDELKMHIPDNARVREQQKRYLKQKSYLHDVTLRAEPYVYWIVEQIKQRK
CCCCCHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHHHHHC
MPMELVLLPIVESAFDPKATSASNAAGLWQIVPRTGKNYGLKQNQWYDGRRDVVASTTVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LDMMQRLNRMFDGDWLLTIAAYNSGEGRIMKAIKRNKARGRPTDFWSLALPRETTIYVPK
HHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHH
MLALSNLIKNSKQFGIALPKSNAQRALARVEVGQQIGLTQAAEMSGLSLTKLKSFNTGYK
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCCCC
RNVTAPNGPHYIMLPKEHASQLRTSLAEGGIAVVQSTQLADNLGGSYEVRAGDTLSGIAQ
CCCCCCCCCEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCEEEECCCHHHHHHH
RLNVKTAELHKWNNLRSARSLKVGQTLKVGSGTMQIANNNSITYQVRKGDSLESIARRHG
HHCCCHHHHHHHHCHHHHHCCCCCCEEEECCCEEEEECCCEEEEEEECCCCHHHHHHHHC
VDIKDVMRWNDALTNANNLQPGLKLTLFVNGKSTDS
CCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]