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The map label for this gene is raiA [H]

Identifier: 85058559

GI number: 85058559

Start: 981052

End: 981381

Strand: Reverse

Name: raiA [H]

Synonym: SG0581

Alternate gene names: 85058559

Gene position: 981381-981052 (Counterclockwise)

Preceding gene: 85058560

Following gene: 85058558

Centisome position: 23.53

GC content: 50.91

Gene sequence:

>330_bases
ATGATAATCAACATTACCGGTAAGCAAATGGATATCACTCCCGCCATTCGTCAACACGCGGAAGAGCGCTTGGCTAAACT
GGCGAAATGGCAGACCCAGCTGATTAATCCCCATATTATCCTCTCTAAACAGCCGCAGGGATTTGTGGCCGATGCCACCC
TGAGCACGCCCAACGGACAGCTGGTGGCCAGTGCAACCCATCAGGATATGTATTTGGCCATCAACGAGCTGCTGGCGAAA
CTTGAACGTCAAATCAATAAAGTCCAGCATAAGGGTGAAGCTCGCCGCGCCGCAGAATGCGTTAAAACTGCGAATTTCAT
GGCGGTGTAA

Upstream 100 bases:

>100_bases
CACCGCCACGCCGAAAAAAAAGTGATCTAAATCACAAAAAAGATACCGGCTGCAGGTATGCTTAAACTATGTCGACGGAA
ACACGCAGAGAGGTAAGTTT

Downstream 100 bases:

>100_bases
TCCTGCCGGCCGGGTAAACCGTCCCGCCCGACAGTGAGCAACACATTGCATACGCGCCCCCGGGCGCGTTTTATATTGAC
AGAAAGAAAAGCGAGCGGTT

Product: putative sigma-54 modulation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MIINITGKQMDITPAIRQHAEERLAKLAKWQTQLINPHIILSKQPQGFVADATLSTPNGQLVASATHQDMYLAINELLAK
LERQINKVQHKGEARRAAECVKTANFMAV

Sequences:

>Translated_109_residues
MIINITGKQMDITPAIRQHAEERLAKLAKWQTQLINPHIILSKQPQGFVADATLSTPNGQLVASATHQDMYLAINELLAK
LERQINKVQHKGEARRAAECVKTANFMAV
>Mature_109_residues
MIINITGKQMDITPAIRQHAEERLAKLAKWQTQLINPHIILSKQPQGFVADATLSTPNGQLVASATHQDMYLAINELLAK
LERQINKVQHKGEARRAAECVKTANFMAV

Specific function: During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored,

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family [H]

Homologues:

Organism=Escherichia coli, GI1788949, Length=107, Percent_Identity=76.6355140186916, Blast_Score=176, Evalue=2e-46,
Organism=Escherichia coli, GI1789595, Length=87, Percent_Identity=37.9310344827586, Blast_Score=65, Evalue=1e-12,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003489 [H]

Pfam domain/function: PF02482 Ribosomal_S30AE [H]

EC number: NA

Molecular weight: Translated: 12141; Mature: 12141

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINITGKQMDITPAIRQHAEERLAKLAKWQTQLINPHIILSKQPQGFVADATLSTPNGQ
CEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECEECCCCCC
LVASATHQDMYLAINELLAKLERQINKVQHKGEARRAAECVKTANFMAV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIINITGKQMDITPAIRQHAEERLAKLAKWQTQLINPHIILSKQPQGFVADATLSTPNGQ
CEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECEECCCCCC
LVASATHQDMYLAINELLAKLERQINKVQHKGEARRAAECVKTANFMAV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]