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The map label for this gene is pheA

Identifier: 85058558

GI number: 85058558

Start: 979608

End: 980765

Strand: Reverse

Name: pheA

Synonym: SG0580

Alternate gene names: 85058558

Gene position: 980765-979608 (Counterclockwise)

Preceding gene: 85058559

Following gene: 85058537

Centisome position: 23.51

GC content: 53.97

Gene sequence:

>1158_bases
ATGAATGACAACACTTTATTGACGCTGCGCGATCGGATTAGCGCGCTGGATCTCAAATTGCTGTCTTTACTCGCGGAAAG
GCGCGCTCTGGCCCTGGACGTCGCGTGGAGCAAGATAGCCTCCCAGCGGCCAATACGCGATATGGCGAGGGAGCGCGAAC
TGCTAAAACGCCTGGTCGCGGAGGGTCAGAAATGTGATCTGGATGGCTTCTATGTGACCCGGCTGTTCCAAATGATTATT
GAAGATTCGGTATTAACGCAGCAAGCCCTACTGCAGTTGGATCTTAATCACCAGGAGCAGCTTTCGGCGCGCATCGCCTT
TCTTGGCCCCAAAGGCTCTTATTCTCACCTGGCGGCCCGGCAGTACGGCGCGCGCTATTTTGAACAGGTCGTCGAATGCA
GTTGCCAAAAACTTGCCGATATTGTGCAACTCGTGGAAACGGGCCAAGCGGAATACGGTATTTTTCCTATCGAAAATAGT
AGCTCAGGTTCCATCAATGAAGTGTACGACCTGCTGCAACACACGCATCTGTCTTTAGTGGGCGAAATCGCTATCCCTAT
CGATCATTGCGTTCTGGTCAGCGGCAATACCAATCTTGATCGTATCGAGGTCATCTACAGCCATCCGCAACCTTTCCAGC
AGTGCAGTCAGTTTCTGGGACATTTTCCTCAGTGGCAATTTGAATATTGCGAAAGCACCGCCGCGGCCATGGAAAAAGTG
GCGCAGCTCAACCGGCCTACGGTCGCCGCGCTAGGCAGCGGCCAGGGTGGCGCCTTATACGGCCTGCAGGTGCTGGAAAG
CAACTTGGCCAACCAGCAGCAAAATATCACCCGCTTTATCGTACTGGCCCGCAAACCTATCGACGTCACCGAGCAGGTGC
CGGCTAAAACCACTCTTATCATGGCTACCGGCCAACAGTCCGGCTCCCTGGTTGAGGCGCTGTTGGTGCTGCGGACCCAC
GGTATTGTGATGACGCGTCTGGAATCCCGGCCGATACACGGTAATGCGCATGAAGAGATGTTTTATATCGATGTGCAGGC
CAATTTGCGCAGCGTGAAAATGCAAAAAGCGCTGCGCGAACTGCAAGCCATTACGCGTTCGTTAAAAGTGCTAGGCTGCT
ACCCCAGCGAGAACGTTTTGCCGGTGGATCCGGAGTAA

Upstream 100 bases:

>100_bases
AGAGGCCCGCGCTCGTCGTGTGAGAAATAAAGCGAAGACGAACCGCAAAGCCTCCTGAAACAGGGGGCTTTTTTTGCTGG
CGTGAAAGGTAACCCATATT

Downstream 100 bases:

>100_bases
CCGGCACCGGTAATCAGCCACTTGTCATTAGCGACTTGTGACCTGCAAGGCGCCGCCGCCGTGCTTCCCGTCTCCCCGTC
CTACGCCTGTTTACGGCGAG

Product: bifunctional chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MNDNTLLTLRDRISALDLKLLSLLAERRALALDVAWSKIASQRPIRDMARERELLKRLVAEGQKCDLDGFYVTRLFQMII
EDSVLTQQALLQLDLNHQEQLSARIAFLGPKGSYSHLAARQYGARYFEQVVECSCQKLADIVQLVETGQAEYGIFPIENS
SSGSINEVYDLLQHTHLSLVGEIAIPIDHCVLVSGNTNLDRIEVIYSHPQPFQQCSQFLGHFPQWQFEYCESTAAAMEKV
AQLNRPTVAALGSGQGGALYGLQVLESNLANQQQNITRFIVLARKPIDVTEQVPAKTTLIMATGQQSGSLVEALLVLRTH
GIVMTRLESRPIHGNAHEEMFYIDVQANLRSVKMQKALRELQAITRSLKVLGCYPSENVLPVDPE

Sequences:

>Translated_385_residues
MNDNTLLTLRDRISALDLKLLSLLAERRALALDVAWSKIASQRPIRDMARERELLKRLVAEGQKCDLDGFYVTRLFQMII
EDSVLTQQALLQLDLNHQEQLSARIAFLGPKGSYSHLAARQYGARYFEQVVECSCQKLADIVQLVETGQAEYGIFPIENS
SSGSINEVYDLLQHTHLSLVGEIAIPIDHCVLVSGNTNLDRIEVIYSHPQPFQQCSQFLGHFPQWQFEYCESTAAAMEKV
AQLNRPTVAALGSGQGGALYGLQVLESNLANQQQNITRFIVLARKPIDVTEQVPAKTTLIMATGQQSGSLVEALLVLRTH
GIVMTRLESRPIHGNAHEEMFYIDVQANLRSVKMQKALRELQAITRSLKVLGCYPSENVLPVDPE
>Mature_385_residues
MNDNTLLTLRDRISALDLKLLSLLAERRALALDVAWSKIASQRPIRDMARERELLKRLVAEGQKCDLDGFYVTRLFQMII
EDSVLTQQALLQLDLNHQEQLSARIAFLGPKGSYSHLAARQYGARYFEQVVECSCQKLADIVQLVETGQAEYGIFPIENS
SSGSINEVYDLLQHTHLSLVGEIAIPIDHCVLVSGNTNLDRIEVIYSHPQPFQQCSQFLGHFPQWQFEYCESTAAAMEKV
AQLNRPTVAALGSGQGGALYGLQVLESNLANQQQNITRFIVLARKPIDVTEQVPAKTTLIMATGQQSGSLVEALLVLRTH
GIVMTRLESRPIHGNAHEEMFYIDVQANLRSVKMQKALRELQAITRSLKVLGCYPSENVLPVDPE

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=383, Percent_Identity=69.4516971279373, Blast_Score=558, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6324013, Length=327, Percent_Identity=27.5229357798165, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 43024; Mature: 43024

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNTLLTLRDRISALDLKLLSLLAERRALALDVAWSKIASQRPIRDMARERELLKRLVA
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGQKCDLDGFYVTRLFQMIIEDSVLTQQALLQLDLNHQEQLSARIAFLGPKGSYSHLAAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCHHHHHHH
QYGARYFEQVVECSCQKLADIVQLVETGQAEYGIFPIENSSSGSINEVYDLLQHTHLSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
GEIAIPIDHCVLVSGNTNLDRIEVIYSHPQPFQQCSQFLGHFPQWQFEYCESTAAAMEKV
HHHEECEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQLNRPTVAALGSGQGGALYGLQVLESNLANQQQNITRFIVLARKPIDVTEQVPAKTTLI
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHCCCCCEEEE
MATGQQSGSLVEALLVLRTHGIVMTRLESRPIHGNAHEEMFYIDVQANLRSVKMQKALRE
EEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
LQAITRSLKVLGCYPSENVLPVDPE
HHHHHHHHHEEEECCCCCEECCCCC
>Mature Secondary Structure
MNDNTLLTLRDRISALDLKLLSLLAERRALALDVAWSKIASQRPIRDMARERELLKRLVA
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EGQKCDLDGFYVTRLFQMIIEDSVLTQQALLQLDLNHQEQLSARIAFLGPKGSYSHLAAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCCCCHHHHHHH
QYGARYFEQVVECSCQKLADIVQLVETGQAEYGIFPIENSSSGSINEVYDLLQHTHLSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
GEIAIPIDHCVLVSGNTNLDRIEVIYSHPQPFQQCSQFLGHFPQWQFEYCESTAAAMEKV
HHHEECEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQLNRPTVAALGSGQGGALYGLQVLESNLANQQQNITRFIVLARKPIDVTEQVPAKTTLI
HHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHCCCCCEEEE
MATGQQSGSLVEALLVLRTHGIVMTRLESRPIHGNAHEEMFYIDVQANLRSVKMQKALRE
EEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
LQAITRSLKVLGCYPSENVLPVDPE
HHHHHHHHHEEEECCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512561; 1444388 [H]