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The map label for this gene is leuA

Identifier: 85058415

GI number: 85058415

Start: 767035

End: 768612

Strand: Reverse

Name: leuA

Synonym: SG0437

Alternate gene names: 85058415

Gene position: 768612-767035 (Counterclockwise)

Preceding gene: 85058417

Following gene: 162382797

Centisome position: 18.43

GC content: 57.1

Gene sequence:

>1578_bases
ATGAGCCAACAAGTCATTATTTTCGATACGACGCTGCGTGATGGTGAACAGGCGCTGCAGGCGAGCCTGAGCGTAAAAGA
GAAACTGCAGGTTGCGCAGGCGCTGGAAAGAATGGGCGTTGACGTCATGGAGGTGGGCTTTCCGGTGTCTTCCCCCGGCG
ATTTCGAGTCGGTGCAAACCATTGCCCGCCAGATCAAAAACAGCCGGGTTTGCGGCCTGGCGCGCTGCGTCGATCGCGAT
ATTGATGTCGCGGCCGAGGCGCTGCGCGTGGCGGAAGCGTTCCGTATCCATTTGTTCCTGGCCACCTCGACCCTGCATGT
GGAATCCAAGCTAAAAAAGAGCTTCGACCAGGTGGTCGAAATGGCGGTGCACTCTGTTAAACGTGCGCGCAACTATACCG
ATGACGTCGAATTTTCCTGCGAGGACGCCGGCCGCACCCCGATCGACAACCTGTGCCGTATTGTTGAAGCCACCATTAAA
GCTGGTGCGCGCACCATCAATATTCCCGATACGGTGGGCTACACCACGCCGAATCAGTTCGGCGGCATTATCAGTTCCCT
GTATCAGCACGTGCCAAATATCGATAAGGCCATCATCTCGGTCCATTGCCACGACGACCTGGGCATGGCGGTCGGCAACT
CTATCGCCGCAATTCAGTCCGGCGCCCGTCAAGTGGAAGGGACGCTGAACGGCATCGGCGAACGCGCCGGCAATACCGCG
CTGGAAGAGGTAATCATGGCGATCAAAGTGCGCCAGGATCTGCTGAACGTCCACACTGGCATCCATCATCAGGAAATCTA
CCGCACCAGCCAGGTAGTCAGCCAGCTGTGCAACATGCCTATCCCCGCCAATAAAGCAGTAGTGGGCGCCAATGCCTTCG
CTCACTCTTCCGGTATTCATCAGGACGGCGTGCTGAAGAATCGCCAGAACTACGAAATCATGACACCGGAAATCATCGGC
CTGAAAGAAGTGCAGTTGAATCTGACCTCCCGCTCCGGTCGCGCGGCGGTGAAACACCGCATGCAGGAGATGGGCTATCA
GGAAAGCAATTACTCCCTGGACGAACTGTATGACGCCTTCCTGAAACTCGCCGATAAAAAGGGGCAGGTCTTCGATTACG
ATCTGGAAGCGCTGGCGTTTATCAATAATCAGCAGGAGCAATCGGAGCATTTCCGCCTGGAATACTTCAGCGTGCAATCC
AGCTCCGCCGATATCGCCACCGCCTCGGTGAAACTGGCCTTCGGTGACGAGGTGCACGCCGAAGCCGCTACCGGCAACGG
CCCGGTAGATGCCGTCTACGAGGCGCTGAACCGCATTACGCAACTCCCCATTCAGTTGGAAAAGTATCAGTTGACCGCCA
AGGGTCACGGCCGTGACGCGCTGGGCCAGGTGGATATCGTCGTGGAGTATGAGGGTCGCCGTTTCCACGGCGTCGGTCTG
GCCACGGATATCATCGAATCCTCCGCCCTGGCGATGGTCAATTCGATGAACACTATCTGGCGCGCGCGGCAAGTCGGTAT
CGAATTGCGGCGCCTGCACCAACACAACAACAGTCAGGATATGCAGGAAACGGTGTAA

Upstream 100 bases:

>100_bases
CAGAACTGAATTCGGGCCACTCCAGATTAAAACCCGCGCACTACTGCGCGGGTTTTTTTATGCCTGGCGTGTGCGGAAAC
CAACAGAATAAGGATCGACC

Downstream 100 bases:

>100_bases
TGATGAGCAAGACCTACCATATTGCGGTTTTACCCGGCGACGGTATCGGCCCGGAAGTCATGGCGCAGGCCTATAAGATT
CTTGATGCGGTGCGCCAGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQVAQALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDRD
IDVAAEALRVAEAFRIHLFLATSTLHVESKLKKSFDQVVEMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEATIK
AGARTINIPDTVGYTTPNQFGGIISSLYQHVPNIDKAIISVHCHDDLGMAVGNSIAAIQSGARQVEGTLNGIGERAGNTA
LEEVIMAIKVRQDLLNVHTGIHHQEIYRTSQVVSQLCNMPIPANKAVVGANAFAHSSGIHQDGVLKNRQNYEIMTPEIIG
LKEVQLNLTSRSGRAAVKHRMQEMGYQESNYSLDELYDAFLKLADKKGQVFDYDLEALAFINNQQEQSEHFRLEYFSVQS
SSADIATASVKLAFGDEVHAEAATGNGPVDAVYEALNRITQLPIQLEKYQLTAKGHGRDALGQVDIVVEYEGRRFHGVGL
ATDIIESSALAMVNSMNTIWRARQVGIELRRLHQHNNSQDMQETV

Sequences:

>Translated_525_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQVAQALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDRD
IDVAAEALRVAEAFRIHLFLATSTLHVESKLKKSFDQVVEMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEATIK
AGARTINIPDTVGYTTPNQFGGIISSLYQHVPNIDKAIISVHCHDDLGMAVGNSIAAIQSGARQVEGTLNGIGERAGNTA
LEEVIMAIKVRQDLLNVHTGIHHQEIYRTSQVVSQLCNMPIPANKAVVGANAFAHSSGIHQDGVLKNRQNYEIMTPEIIG
LKEVQLNLTSRSGRAAVKHRMQEMGYQESNYSLDELYDAFLKLADKKGQVFDYDLEALAFINNQQEQSEHFRLEYFSVQS
SSADIATASVKLAFGDEVHAEAATGNGPVDAVYEALNRITQLPIQLEKYQLTAKGHGRDALGQVDIVVEYEGRRFHGVGL
ATDIIESSALAMVNSMNTIWRARQVGIELRRLHQHNNSQDMQETV
>Mature_524_residues
SQQVIIFDTTLRDGEQALQASLSVKEKLQVAQALERMGVDVMEVGFPVSSPGDFESVQTIARQIKNSRVCGLARCVDRDI
DVAAEALRVAEAFRIHLFLATSTLHVESKLKKSFDQVVEMAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEATIKA
GARTINIPDTVGYTTPNQFGGIISSLYQHVPNIDKAIISVHCHDDLGMAVGNSIAAIQSGARQVEGTLNGIGERAGNTAL
EEVIMAIKVRQDLLNVHTGIHHQEIYRTSQVVSQLCNMPIPANKAVVGANAFAHSSGIHQDGVLKNRQNYEIMTPEIIGL
KEVQLNLTSRSGRAAVKHRMQEMGYQESNYSLDELYDAFLKLADKKGQVFDYDLEALAFINNQQEQSEHFRLEYFSVQSS
SADIATASVKLAFGDEVHAEAATGNGPVDAVYEALNRITQLPIQLEKYQLTAKGHGRDALGQVDIVVEYEGRRFHGVGLA
TDIIESSALAMVNSMNTIWRARQVGIELRRLHQHNNSQDMQETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=298, Percent_Identity=23.489932885906, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786261, Length=525, Percent_Identity=78.4761904761905, Blast_Score=869, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=26.8022181146026, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=28.0555555555556, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=360, Percent_Identity=28.0555555555556, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=25.3731343283582, Blast_Score=136, Evalue=9e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SODGM (Q2NVW3)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454117.1
- ProteinModelPortal:   Q2NVW3
- SMR:   Q2NVW3
- STRING:   Q2NVW3
- GeneID:   3867412
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0437
- NMPDR:   fig|343509.6.peg.1007
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q2NVW3
- ProtClustDB:   PRK00915
- BioCyc:   SGLO343509:SG0437-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57870; Mature: 57739

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSVKEKLQVAQALERMGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHH
IARQIKNSRVCGLARCVDRDIDVAAEALRVAEAFRIHLFLATSTLHVESKLKKSFDQVVE
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHH
MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEATIKAGARTINIPDTVGYTTPNQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIISSLYQHVPNIDKAIISVHCHDDLGMAVGNSIAAIQSGARQVEGTLNGIGERAGNTA
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LEEVIMAIKVRQDLLNVHTGIHHQEIYRTSQVVSQLCNMPIPANKAVVGANAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHCCCCC
QDGVLKNRQNYEIMTPEIIGLKEVQLNLTSRSGRAAVKHRMQEMGYQESNYSLDELYDAF
CCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINNQQEQSEHFRLEYFSVQSSSADIATASVKLAFGDEVHA
HHHHCCCCCEEECCHHHHHHHCCCHHHHHCEEEEEEEECCCCCCEEEEEEEEEECCCCCC
EAATGNGPVDAVYEALNRITQLPIQLEKYQLTAKGHGRDALGQVDIVVEYEGRRFHGVGL
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCEEECCCH
ATDIIESSALAMVNSMNTIWRARQVGIELRRLHQHNNSQDMQETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSVKEKLQVAQALERMGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHH
IARQIKNSRVCGLARCVDRDIDVAAEALRVAEAFRIHLFLATSTLHVESKLKKSFDQVVE
HHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHH
MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEATIKAGARTINIPDTVGYTTPNQF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIISSLYQHVPNIDKAIISVHCHDDLGMAVGNSIAAIQSGARQVEGTLNGIGERAGNTA
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LEEVIMAIKVRQDLLNVHTGIHHQEIYRTSQVVSQLCNMPIPANKAVVGANAFAHSSGIH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHCCCCC
QDGVLKNRQNYEIMTPEIIGLKEVQLNLTSRSGRAAVKHRMQEMGYQESNYSLDELYDAF
CCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFINNQQEQSEHFRLEYFSVQSSSADIATASVKLAFGDEVHA
HHHHCCCCCEEECCHHHHHHHCCCHHHHHCEEEEEEEECCCCCCEEEEEEEEEECCCCCC
EAATGNGPVDAVYEALNRITQLPIQLEKYQLTAKGHGRDALGQVDIVVEYEGRRFHGVGL
CCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEEEECCCEEECCCH
ATDIIESSALAMVNSMNTIWRARQVGIELRRLHQHNNSQDMQETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA