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The map label for this gene is leuB

Identifier: 162382797

GI number: 162382797

Start: 765941

End: 767032

Strand: Reverse

Name: leuB

Synonym: SG0436

Alternate gene names: 162382797

Gene position: 767032-765941 (Counterclockwise)

Preceding gene: 85058415

Following gene: 85058413

Centisome position: 18.39

GC content: 61.17

Gene sequence:

>1092_bases
ATGAGCAAGACCTACCATATTGCGGTTTTACCCGGCGACGGTATCGGCCCGGAAGTCATGGCGCAGGCCTATAAGATTCT
TGATGCGGTGCGCCAGCGCTTCAACGTGCGCATTTCCACCAGCGAGTATGATGTGGGCGGCGCGGCTATCGACCGCCAGG
GCAGCCCGCTGCCCGCCGGCACCGTTGCCGGCTGCGAGCAGGCGGATGCGATTCTGTTCGGTTCAGTGGGCGGTCCGAAA
TGGGAACACTTGCCGGCGGCGGAACAGCCGGAGCGCGGCGCCCTGCTGCCGCTGCGTAAACACTTCAAGCTGTTTTCCAA
CCTGCGCCCGTCGCGCCTGTACCCTGGGCTTGAGGCCTATTGCCCGCTGCGCGCCAATATCGCCGAACGCGGATTCGATA
TCTTGTGCGTGCGGGAACTCACCGGTGGCATCTATTTCGGCCAACCGAAAGGCCGCGAAGGCACCGGTCCGCACGAGCAT
GCTTTCGATACCGAGGTGTACTATCGCTTTGAGATTGAACGCATCGCCCGCATAGCTTTTGAATCGGCGCGCAAACGGCG
CGGCAAAGTCACCTCCATCGACAAAGCCAACGTGTTGCAAAGCTCCATTCTGTGGCGCAAGGTGGTCAGTCAGGTGGCAC
CGGACTACCCGGATGTCGCTCTGTCGCATTTGTATATCGACAACGCCACCATGCAATTGATTAAAGATCCGTCCCAGTTC
GATGTGATGCTGTGCTCGAATCTGTTCGGCGATATTCTCTCCGACGAATGCGCCATGATCACCGGCTCCATGGGCATGCT
GCCTTCGGCCAGCCTGAATGAGCAGGGCTTCGGCATGTATGAGCCGGCCGGTGGCTCGGCGCCGGATATCGCCGGCAAGG
ATATCGCCAACCCGGTGGCACAGATTTTGTCCGCCTCCCTGCTGCTGCGCTACAGCCTGGGGCTGGACGACGCCGCGGAC
GCGATTGAGCTCGCGGTTAATCAGGCGCTGGAAGCCGGACACCGCACCGCCGACCTGGCCGGCGGCGGCAGCGCGGTAGG
TACCGGTGAAATGGGCGATATTATCGCCGCGTTAATCAGTCAAGGGGCATAA

Upstream 100 bases:

>100_bases
ATTCGATGAACACTATCTGGCGCGCGCGGCAAGTCGGTATCGAATTGCGGCGCCTGCACCAACACAACAACAGTCAGGAT
ATGCAGGAAACGGTGTAATG

Downstream 100 bases:

>100_bases
TCAACATGGGCAAGTCGTTATACCAAAAATTATATGATGCACACGTGGTTTATGAAGCGGAGAACGAAACGCCGCTTTTG
TATATCGACCGCCATTTGGT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSKTYHIAVLPGDGIGPEVMAQAYKILDAVRQRFNVRISTSEYDVGGAAIDRQGSPLPAGTVAGCEQADAILFGSVGGPK
WEHLPAAEQPERGALLPLRKHFKLFSNLRPSRLYPGLEAYCPLRANIAERGFDILCVRELTGGIYFGQPKGREGTGPHEH
AFDTEVYYRFEIERIARIAFESARKRRGKVTSIDKANVLQSSILWRKVVSQVAPDYPDVALSHLYIDNATMQLIKDPSQF
DVMLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGMYEPAGGSAPDIAGKDIANPVAQILSASLLLRYSLGLDDAAD
AIELAVNQALEAGHRTADLAGGGSAVGTGEMGDIIAALISQGA

Sequences:

>Translated_363_residues
MSKTYHIAVLPGDGIGPEVMAQAYKILDAVRQRFNVRISTSEYDVGGAAIDRQGSPLPAGTVAGCEQADAILFGSVGGPK
WEHLPAAEQPERGALLPLRKHFKLFSNLRPSRLYPGLEAYCPLRANIAERGFDILCVRELTGGIYFGQPKGREGTGPHEH
AFDTEVYYRFEIERIARIAFESARKRRGKVTSIDKANVLQSSILWRKVVSQVAPDYPDVALSHLYIDNATMQLIKDPSQF
DVMLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGMYEPAGGSAPDIAGKDIANPVAQILSASLLLRYSLGLDDAAD
AIELAVNQALEAGHRTADLAGGGSAVGTGEMGDIIAALISQGA
>Mature_362_residues
SKTYHIAVLPGDGIGPEVMAQAYKILDAVRQRFNVRISTSEYDVGGAAIDRQGSPLPAGTVAGCEQADAILFGSVGGPKW
EHLPAAEQPERGALLPLRKHFKLFSNLRPSRLYPGLEAYCPLRANIAERGFDILCVRELTGGIYFGQPKGREGTGPHEHA
FDTEVYYRFEIERIARIAFESARKRRGKVTSIDKANVLQSSILWRKVVSQVAPDYPDVALSHLYIDNATMQLIKDPSQFD
VMLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGMYEPAGGSAPDIAGKDIANPVAQILSASLLLRYSLGLDDAADA
IELAVNQALEAGHRTADLAGGGSAVGTGEMGDIIAALISQGA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=346, Percent_Identity=34.393063583815, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI28178821, Length=371, Percent_Identity=28.0323450134771, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI28178816, Length=366, Percent_Identity=28.6885245901639, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4758582, Length=349, Percent_Identity=27.5071633237822, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=27.9635258358663, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI28178819, Length=204, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=81.4917127071823, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1788101, Length=373, Percent_Identity=36.9973190348526, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787381, Length=401, Percent_Identity=25.9351620947631, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=34.1107871720117, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=31.728045325779, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=29.4943820224719, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=43.2795698924731, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=33.5195530726257, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=354, Percent_Identity=31.9209039548023, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=364, Percent_Identity=29.3956043956044, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24643268, Length=353, Percent_Identity=33.9943342776204, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24643270, Length=353, Percent_Identity=33.9943342776204, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24661184, Length=351, Percent_Identity=30.4843304843305, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078633, Length=349, Percent_Identity=29.512893982808, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650122, Length=349, Percent_Identity=29.512893982808, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078635, Length=349, Percent_Identity=29.7994269340974, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078637, Length=349, Percent_Identity=29.512893982808, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078639, Length=347, Percent_Identity=29.6829971181556, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=26.7241379310345, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=26.7241379310345, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=22.2857142857143, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_SODGM (Q2NVW4)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454116.2
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2NVW4
- SMR:   Q2NVW4
- STRING:   Q2NVW4
- GeneID:   3867411
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0436
- NMPDR:   fig|343509.6.peg.1006
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q2NVW4
- ProtClustDB:   PRK00772
- BioCyc:   SGLO343509:SG0436-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38854; Mature: 38722

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 100-100 BINDING 110-110 BINDING 139-139 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTYHIAVLPGDGIGPEVMAQAYKILDAVRQRFNVRISTSEYDVGGAAIDRQGSPLPAG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEECCCCCCCCCC
TVAGCEQADAILFGSVGGPKWEHLPAAEQPERGALLPLRKHFKLFSNLRPSRLYPGLEAY
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHC
CPLRANIAERGFDILCVRELTGGIYFGQPKGREGTGPHEHAFDTEVYYRFEIERIARIAF
CCCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
ESARKRRGKVTSIDKANVLQSSILWRKVVSQVAPDYPDVALSHLYIDNATMQLIKDPSQF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCHHHHHCCCCHH
DVMLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGMYEPAGGSAPDIAGKDIANPVA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QILSASLLLRYSLGLDDAADAIELAVNQALEAGHRTADLAGGGSAVGTGEMGDIIAALIS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHH
QGA
CCC
>Mature Secondary Structure 
SKTYHIAVLPGDGIGPEVMAQAYKILDAVRQRFNVRISTSEYDVGGAAIDRQGSPLPAG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEECCCCCCCCCC
TVAGCEQADAILFGSVGGPKWEHLPAAEQPERGALLPLRKHFKLFSNLRPSRLYPGLEAY
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHC
CPLRANIAERGFDILCVRELTGGIYFGQPKGREGTGPHEHAFDTEVYYRFEIERIARIAF
CCCHHHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
ESARKRRGKVTSIDKANVLQSSILWRKVVSQVAPDYPDVALSHLYIDNATMQLIKDPSQF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCHHHHHCCCCHH
DVMLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGMYEPAGGSAPDIAGKDIANPVA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QILSASLLLRYSLGLDDAADAIELAVNQALEAGHRTADLAGGGSAVGTGEMGDIIAALIS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHH
QGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA