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The map label for this gene is ksgA

Identifier: 85058402

GI number: 85058402

Start: 746161

End: 746979

Strand: Reverse

Name: ksgA

Synonym: SG0424

Alternate gene names: 85058402

Gene position: 746979-746161 (Counterclockwise)

Preceding gene: 85058403

Following gene: 85058401

Centisome position: 17.91

GC content: 58.49

Gene sequence:

>819_bases
ATGAATAACCGCGTACATCAGGGGCATCTGGCGCGCAAGCGTTTCGGCCAGAACTTTCTGCACGACAGCTCAATCATTGA
TGCTATTGTTGCCGCCATTCATCCGCAGCCCGGCCAGGCAATAGTGGAAATCGGGCCCGGCCTCGGCGCCCTGACCGAAC
CGGTGGCGGAGCGCGTCGATGAGATGACTGTTATCGAGCTCGACCGCGATCTGGCCGCCCGTCTGGCCAGCCATCCTTTT
TTACAGTCCAAGCTGAACATTGTGCAGAAGGACGCGATGACGGTCGATTTTGCCGCGCTGTCCGCCGAACGGGGGCAGCC
GCTGCGGGTCTTCGGTAATCTGCCGTACAATATTTCCACGCCGCTTATGTTTCACCTTTTCAGCTATACTCATGCCATTC
GCGATATGCACTTCATGCTGCAAAAAGAGGTGGTCAACCGACTGGTGGACGGCCCGAACGGCAAGGCCTACGGCCGGTTA
AGCGTCATGGCCCAGTATTACTGCCAGATCATCCCGGTGCTGGAAGTGCCGCCTGCCTCGTTTCGGCCGGCGCCGAAAGT
GGATTCGGCGGTGGTGCGGCTGGTACCCTATGCCACGCTGCCGTTCCCGGTGAAGGATCTGAGCAAACTCGCCACCTTGA
CCAGCCTGGCGTTCAACCAGCGCCGCAAGACGTTGCGTAATAGCCTGGGTAATCTTTTTAGCGCGCAACAGCTGGCGGAA
CAGGGTATCGACGCAACGCTGCGCGCGGAAAATGTGAGCGTTGAGCTTTATTGCCGTTTAGCCAACGTGCTGGCGGAGCG
CACCGTTTCACAAGACTAA

Upstream 100 bases:

>100_bases
GATCACGGCACCGCGCTGGAGCTGGCCGGATCGGGTCAGGCGGAGGCCGGCAGTATCAAAACGGCTCTTAATATCGCCAT
CGACATGATAAAACATCGTA

Downstream 100 bases:

>100_bases
CAGGAGTGCTTTGATGATTAACTCGCCCCGTGTCTGTGTTCAGGTTCAGAGCATGTACATCGAGACGCAGTCTCAGCCGG
AAAGTGATCGTTATGTCTTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNNRVHQGHLARKRFGQNFLHDSSIIDAIVAAIHPQPGQAIVEIGPGLGALTEPVAERVDEMTVIELDRDLAARLASHPF
LQSKLNIVQKDAMTVDFAALSAERGQPLRVFGNLPYNISTPLMFHLFSYTHAIRDMHFMLQKEVVNRLVDGPNGKAYGRL
SVMAQYYCQIIPVLEVPPASFRPAPKVDSAVVRLVPYATLPFPVKDLSKLATLTSLAFNQRRKTLRNSLGNLFSAQQLAE
QGIDATLRAENVSVELYCRLANVLAERTVSQD

Sequences:

>Translated_272_residues
MNNRVHQGHLARKRFGQNFLHDSSIIDAIVAAIHPQPGQAIVEIGPGLGALTEPVAERVDEMTVIELDRDLAARLASHPF
LQSKLNIVQKDAMTVDFAALSAERGQPLRVFGNLPYNISTPLMFHLFSYTHAIRDMHFMLQKEVVNRLVDGPNGKAYGRL
SVMAQYYCQIIPVLEVPPASFRPAPKVDSAVVRLVPYATLPFPVKDLSKLATLTSLAFNQRRKTLRNSLGNLFSAQQLAE
QGIDATLRAENVSVELYCRLANVLAERTVSQD
>Mature_272_residues
MNNRVHQGHLARKRFGQNFLHDSSIIDAIVAAIHPQPGQAIVEIGPGLGALTEPVAERVDEMTVIELDRDLAARLASHPF
LQSKLNIVQKDAMTVDFAALSAERGQPLRVFGNLPYNISTPLMFHLFSYTHAIRDMHFMLQKEVVNRLVDGPNGKAYGRL
SVMAQYYCQIIPVLEVPPASFRPAPKVDSAVVRLVPYATLPFPVKDLSKLATLTSLAFNQRRKTLRNSLGNLFSAQQLAE
QGIDATLRAENVSVELYCRLANVLAERTVSQD

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=244, Percent_Identity=30.327868852459, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI156415992, Length=287, Percent_Identity=31.0104529616725, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=73.8970588235294, Blast_Score=422, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25146882, Length=214, Percent_Identity=33.1775700934579, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25141369, Length=290, Percent_Identity=25.8620689655172, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=214, Percent_Identity=28.9719626168224, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=32.8947368421053, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357273, Length=288, Percent_Identity=29.8611111111111, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SODGM (Q2NVX6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454104.1
- ProteinModelPortal:   Q2NVX6
- SMR:   Q2NVX6
- STRING:   Q2NVX6
- GeneID:   3867535
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0424
- NMPDR:   fig|343509.6.peg.988
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q2NVX6
- ProtClustDB:   PRK00274
- BioCyc:   SGLO343509:SG0424-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30151; Mature: 30151

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 18-18 BINDING 20-20 BINDING 45-45 BINDING 66-66 BINDING 91-91 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA