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The map label for this gene is fcl [C]

Identifier: 85058308

GI number: 85058308

Start: 607050

End: 607973

Strand: Reverse

Name: fcl [C]

Synonym: SG0330

Alternate gene names: 85058308

Gene position: 607973-607050 (Counterclockwise)

Preceding gene: 85058309

Following gene: 85058304

Centisome position: 14.58

GC content: 52.92

Gene sequence:

>924_bases
ATGGAACTGGACGCTAATATCCTTATTACCGGCGCCAATGGCGTTTTGGGCCACGGCCTGTGGCATGCTCTACAGGCGCA
AGGATACTGTCATTTCCTTTGTCCGACCCGCCAGGAATTGAACGCGCTGAACCGGCAACAGGTGGACGATTTCTTTGCTC
ACCATCGGCCGCACTACGTATTCCATCTTGCCTCGCTGGTGTTCGGTCTGCAAGGCAACATGGAAAATCAGTTAAACGCT
ATCGCGACCAATACGTTAATCAATCAGCATTTACTTCTCACCTGTCAGCGATATCCGGTGCGCAAAATCTTTTTTGCCAG
TACCGTTGCCGGCTATGGCTATCCATACCATTCATTGCCGTTAACCGAGCCGTATTTTTTCAGTGGTGCGCCTCACTCCG
GTGAATATGGTTATGGTATGGCAAAGCGCCATGCGCTGGGCTATCTGGAAATCCTTCGGCGGGAATATGATATTGACTAC
TGCTACGGTATTTTTACCAACCTGTTCGGTCCTAACAACCGCTTCGATATCCACTGCGTCCATGTCATCCCCTCGTTAAT
TGAAAAAGCCGCCGCCAGTATCCGGCGAGAGGATCGCTGTCTCCCTGTATGGGGGCGGCTGGAAACCAGCCGCGATTTCA
TGTTTAGCAAAGAGGCCGGCGCGGCGGTGTTGCACGCCTTTCTGCACGGCAGCGGTATGATTAATATCGCCAGCGGATAC
GAATCTACCCTGGCGCAGGTGGTTGCAGCAATCAACCTTTGCTATCAATCCCGCCTGAATATCGTCTGGGACGCCGACGC
GCCCCGCAGCGTCGACATCAGCCACCTGCGCGCACTTGGTTATCACCCCTCGTCCAACCTTAATGATGCCATCGCAAAAA
CGGTCGACTGGCATCGGCAAAATACTGACAGGATACGCAAATGA

Upstream 100 bases:

>100_bases
TTAAGGCGTTTTACAATACTTTCTTTCTCATGCAAAACGGAGCGCTACGTCAGATAAGCCACAGAGAAATAGATGATTAT
ATTTAAACAGGGAGTGGGCA

Downstream 100 bases:

>100_bases
AAAGAATCGCCATCGTATCGACTTTCGCCGAAAGCTGTGGCAACTCTTATTTCACCTAAATATTGGTGAACTCAATGCGC
CGTATGGGCTATCACGTGGA

Product: GDP-L-fucose-synthase

Products: GDP-4-dehydro-6-L-deoxygalactose; guanosine diphosphate fucose; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MELDANILITGANGVLGHGLWHALQAQGYCHFLCPTRQELNALNRQQVDDFFAHHRPHYVFHLASLVFGLQGNMENQLNA
IATNTLINQHLLLTCQRYPVRKIFFASTVAGYGYPYHSLPLTEPYFFSGAPHSGEYGYGMAKRHALGYLEILRREYDIDY
CYGIFTNLFGPNNRFDIHCVHVIPSLIEKAAASIRREDRCLPVWGRLETSRDFMFSKEAGAAVLHAFLHGSGMINIASGY
ESTLAQVVAAINLCYQSRLNIVWDADAPRSVDISHLRALGYHPSSNLNDAIAKTVDWHRQNTDRIRK

Sequences:

>Translated_307_residues
MELDANILITGANGVLGHGLWHALQAQGYCHFLCPTRQELNALNRQQVDDFFAHHRPHYVFHLASLVFGLQGNMENQLNA
IATNTLINQHLLLTCQRYPVRKIFFASTVAGYGYPYHSLPLTEPYFFSGAPHSGEYGYGMAKRHALGYLEILRREYDIDY
CYGIFTNLFGPNNRFDIHCVHVIPSLIEKAAASIRREDRCLPVWGRLETSRDFMFSKEAGAAVLHAFLHGSGMINIASGY
ESTLAQVVAAINLCYQSRLNIVWDADAPRSVDISHLRALGYHPSSNLNDAIAKTVDWHRQNTDRIRK
>Mature_307_residues
MELDANILITGANGVLGHGLWHALQAQGYCHFLCPTRQELNALNRQQVDDFFAHHRPHYVFHLASLVFGLQGNMENQLNA
IATNTLINQHLLLTCQRYPVRKIFFASTVAGYGYPYHSLPLTEPYFFSGAPHSGEYGYGMAKRHALGYLEILRREYDIDY
CYGIFTNLFGPNNRFDIHCVHVIPSLIEKAAASIRREDRCLPVWGRLETSRDFMFSKEAGAAVLHAFLHGSGMINIASGY
ESTLAQVVAAINLCYQSRLNIVWDADAPRSVDISHLRALGYHPSSNLNDAIAKTVDWHRQNTDRIRK

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=316, Percent_Identity=23.7341772151899, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788365, Length=319, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17554428, Length=320, Percent_Identity=23.4375, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922778, Length=320, Percent_Identity=24.6875, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271 [C]

Molecular weight: Translated: 34691; Mature: 34691

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDANILITGANGVLGHGLWHALQAQGYCHFLCPTRQELNALNRQQVDDFFAHHRPHYV
CCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHH
FHLASLVFGLQGNMENQLNAIATNTLINQHLLLTCQRYPVRKIFFASTVAGYGYPYHSLP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCC
LTEPYFFSGAPHSGEYGYGMAKRHALGYLEILRREYDIDYCYGIFTNLFGPNNRFDIHCV
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEHH
HVIPSLIEKAAASIRREDRCLPVWGRLETSRDFMFSKEAGAAVLHAFLHGSGMINIASGY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEECCCH
ESTLAQVVAAINLCYQSRLNIVWDADAPRSVDISHLRALGYHPSSNLNDAIAKTVDWHRQ
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
NTDRIRK
CHHHHCC
>Mature Secondary Structure
MELDANILITGANGVLGHGLWHALQAQGYCHFLCPTRQELNALNRQQVDDFFAHHRPHYV
CCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHH
FHLASLVFGLQGNMENQLNAIATNTLINQHLLLTCQRYPVRKIFFASTVAGYGYPYHSLP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCC
LTEPYFFSGAPHSGEYGYGMAKRHALGYLEILRREYDIDYCYGIFTNLFGPNNRFDIHCV
CCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEHH
HVIPSLIEKAAASIRREDRCLPVWGRLETSRDFMFSKEAGAAVLHAFLHGSGMINIASGY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEECCCH
ESTLAQVVAAINLCYQSRLNIVWDADAPRSVDISHLRALGYHPSSNLNDAIAKTVDWHRQ
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
NTDRIRK
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-4-dehydro-6-deoxy-D-mannose; GDP-4-dehydro-6-L-deoxygalactose; NADPH; Proton [C]

Specific reaction: GDP-4-dehydro-6-deoxy-D-mannose = GDP-4-dehydro-6-L-deoxygalactose GDP-4-dehydro-6-L-deoxygalactose + NADPH + Proton = guanosine diphosphate fucose + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]