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The map label for this gene is rfbD [H]

Identifier: 85058309

GI number: 85058309

Start: 610853

End: 611698

Strand: Reverse

Name: rfbD [H]

Synonym: SG0331

Alternate gene names: 85058309

Gene position: 611698-610853 (Counterclockwise)

Preceding gene: 85058333

Following gene: 85058308

Centisome position: 14.66

GC content: 48.7

Gene sequence:

>846_bases
TTGAAAAAGAAAGTCGCCATTATTACTGGTATTACTGGCCAAGATGGTTCGTATCTTGCCGAATTCCTATTGAACAAAGG
GTATGAGGTCCATGGCATAAAGCGACGATGCTCGCAGTTTAATACACAGCGTATAGAACATATTTTCCAGACCTCTCATG
ACAATAACACAATGCTTAAATTGCATCATAGCGATCCTTTTGATACACCAAATTTAACCCGCATATTAGCGAAAGTCCAA
CCGGACGAAGTTTACAATCTGGGTGCGCAATCCCACGTCGCGGTCAGTTTTGATTCGCCCGAATATACCGCCGACGTCGA
TACCATAGGCGTATTGCGCTTATTAGAAGCTATCCGTTTTCTTGGCCTCACCGAAAAGACCCGATACTACCAGGCATCCA
CATCGGAGCTTTTCGGTCTGGTATGCGAACCCCGGCAGAAAGAAACTACGCCGTTCTATCCCCGCAGTCCTTATGCTGTG
GCCAAAATGTACGCCTACTGGATAACGGTGAATTATCGGGAAGCCTACGGCATCTACGCCTGCAACGGTATATTATTTAA
CCATGAATCTCCGCGCCGCGGTGAAACGTCCGTTACCCGCAAAATCACCCGCGGCCTGGCTAGTATTGCGCACGATCTGG
AAAAATGCCTGTATATGGACAACCTCGGTGCCCTGCGCGACTGGGGTCATGCACGGGATTATGTCCGCATGCAATGGATG
CTGCTGCAACAGGACAAACCTGAGGATTTCGTCATTGCCACCGGTAACCAGTATACGTTCGGGATTTTATTACCATGGCG
GCGCGGGAACTGGGCATTGAGATTGTTTTTGAGGGCCAAGGCATAA

Upstream 100 bases:

>100_bases
AGAAACTTTATATTTCACATTACTAGCTATGGATTAAGATAATAAAGAGCTACACTTGCCCAATCTTGACACCTCGACCA
TGATACCTTCAGGAGTTATT

Downstream 100 bases:

>100_bases
ATGAGAAAGCCTTTGTCAGCAAAATTACCGGTGGACTTGCCCCCTCGTTACGCGTCGGCGATGTTATTGTTGAAGTGGAC
GAGCGTTATTTCCGGCCTAC

Product: putative GDP-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKKKVAIITGITGQDGSYLAEFLLNKGYEVHGIKRRCSQFNTQRIEHIFQTSHDNNTMLKLHHSDPFDTPNLTRILAKVQ
PDEVYNLGAQSHVAVSFDSPEYTADVDTIGVLRLLEAIRFLGLTEKTRYYQASTSELFGLVCEPRQKETTPFYPRSPYAV
AKMYAYWITVNYREAYGIYACNGILFNHESPRRGETSVTRKITRGLASIAHDLEKCLYMDNLGALRDWGHARDYVRMQWM
LLQQDKPEDFVIATGNQYTFGILLPWRRGNWALRLFLRAKA

Sequences:

>Translated_281_residues
MKKKVAIITGITGQDGSYLAEFLLNKGYEVHGIKRRCSQFNTQRIEHIFQTSHDNNTMLKLHHSDPFDTPNLTRILAKVQ
PDEVYNLGAQSHVAVSFDSPEYTADVDTIGVLRLLEAIRFLGLTEKTRYYQASTSELFGLVCEPRQKETTPFYPRSPYAV
AKMYAYWITVNYREAYGIYACNGILFNHESPRRGETSVTRKITRGLASIAHDLEKCLYMDNLGALRDWGHARDYVRMQWM
LLQQDKPEDFVIATGNQYTFGILLPWRRGNWALRLFLRAKA
>Mature_281_residues
MKKKVAIITGITGQDGSYLAEFLLNKGYEVHGIKRRCSQFNTQRIEHIFQTSHDNNTMLKLHHSDPFDTPNLTRILAKVQ
PDEVYNLGAQSHVAVSFDSPEYTADVDTIGVLRLLEAIRFLGLTEKTRYYQASTSELFGLVCEPRQKETTPFYPRSPYAV
AKMYAYWITVNYREAYGIYACNGILFNHESPRRGETSVTRKITRGLASIAHDLEKCLYMDNLGALRDWGHARDYVRMQWM
LLQQDKPEDFVIATGNQYTFGILLPWRRGNWALRLFLRAKA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=259, Percent_Identity=62.1621621621622, Blast_Score=335, Evalue=2e-92,
Organism=Escherichia coli, GI1788366, Length=256, Percent_Identity=77.34375, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI48994969, Length=250, Percent_Identity=26, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788353, Length=259, Percent_Identity=24.7104247104247, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=259, Percent_Identity=63.3204633204633, Blast_Score=351, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI17539422, Length=259, Percent_Identity=63.3204633204633, Blast_Score=350, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI133901788, Length=259, Percent_Identity=63.3204633204633, Blast_Score=350, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI133901786, Length=259, Percent_Identity=63.3204633204633, Blast_Score=350, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI133901790, Length=259, Percent_Identity=63.3204633204633, Blast_Score=350, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI17507723, Length=260, Percent_Identity=63.0769230769231, Blast_Score=349, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24158427, Length=260, Percent_Identity=61.5384615384615, Blast_Score=336, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 32398; Mature: 32398

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVAIITGITGQDGSYLAEFLLNKGYEVHGIKRRCSQFNTQRIEHIFQTSHDNNTMLK
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LHHSDPFDTPNLTRILAKVQPDEVYNLGAQSHVAVSFDSPEYTADVDTIGVLRLLEAIRF
EECCCCCCCCHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LGLTEKTRYYQASTSELFGLVCEPRQKETTPFYPRSPYAVAKMYAYWITVNYREAYGIYA
HCCCCHHHEEECCHHHHHHEECCCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHCCCEEE
CNGILFNHESPRRGETSVTRKITRGLASIAHDLEKCLYMDNLGALRDWGHARDYVRMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHEEE
LLQQDKPEDFVIATGNQYTFGILLPWRRGNWALRLFLRAKA
EECCCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEEEECC
>Mature Secondary Structure
MKKKVAIITGITGQDGSYLAEFLLNKGYEVHGIKRRCSQFNTQRIEHIFQTSHDNNTMLK
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LHHSDPFDTPNLTRILAKVQPDEVYNLGAQSHVAVSFDSPEYTADVDTIGVLRLLEAIRF
EECCCCCCCCHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LGLTEKTRYYQASTSELFGLVCEPRQKETTPFYPRSPYAVAKMYAYWITVNYREAYGIYA
HCCCCHHHEEECCHHHHHHEECCCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHCCCEEE
CNGILFNHESPRRGETSVTRKITRGLASIAHDLEKCLYMDNLGALRDWGHARDYVRMQWM
ECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHEEE
LLQQDKPEDFVIATGNQYTFGILLPWRRGNWALRLFLRAKA
EECCCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]