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The map label for this gene is lutR [H]

Identifier: 85058151

GI number: 85058151

Start: 314962

End: 315669

Strand: Reverse

Name: lutR [H]

Synonym: SG0173

Alternate gene names: 85058151

Gene position: 315669-314962 (Counterclockwise)

Preceding gene: 85058156

Following gene: 85058149

Centisome position: 7.57

GC content: 48.87

Gene sequence:

>708_bases
ATGGACGTTATCGCACTTAAGAAAGTGACCCTCACCGAACAGATAATGGAGCAAATCGCCTCGCAAATTACCTCAGGCAA
GCTCAAACCCGGTCAGCGTCTGCCAAACGAACGCGCAATGGGCGAAATGTTTAACGTTACCCGTAGCCGCGTGCGTGAGG
CGCTACGTGCCCTTTCGTTGATTGGCTTAATCGAGATAAAACCCGGCGATGGCAGCTATGTCTGCGAAAACAAGCCGCAT
TTACCGGAAGAAACCATAACCTGGATTTTCAACAAGGAAATTCATAATCTGGATGAAGTCTATGCCGCCCGTAAATTGAT
AGAAGAAGAGGTCTTCCTCACGGCGGTAGACACCGCCAGCAATGATGACATCATCCGCCTGCAGTCCATTATGGACAACA
TGACGGAAGTGCCGCTAGCGGGCGGCAAGCCGGAAGATTATCATCTTCTACTGGACGAGTATGATCTGTTAATGGGCAGG
ATCTTCGGCAACCAGATCTACGACAAGCTGATGCAAACCATTATCATGTTGCGCAAGGACTTTTCCCACAAGCTCTTAAA
GGCAGAGGACGCGATGGCGCATAGCCTGAAACTTCGCCTGAAGATTCATGACGCGTTTGTCGAGCGCGATAAAGCGCAAT
TGAAAAAAAACTTGCACACCTTTTTTCAGTCATCACGCAAATTCTACGACAAGATGGCGGACAACTAA

Upstream 100 bases:

>100_bases
CAAAACACCGCAGCCGTCCCGCGCGGCGCCAAGACCATTGCACAACGGGGCGCGATACCCTAAATTCATGGACATGAACA
CTGTCCCTGCCGGAGGGAAA

Downstream 100 bases:

>100_bases
GAAAACGCTATGGCTAAAGTAAAATCGGCGGAAAGAACCTTCCGGCTCGTGGAACTCATCGCCAGCCATCGCGAGGGGCT
GACTTTTTCTCAGTTGCAGG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MDVIALKKVTLTEQIMEQIASQITSGKLKPGQRLPNERAMGEMFNVTRSRVREALRALSLIGLIEIKPGDGSYVCENKPH
LPEETITWIFNKEIHNLDEVYAARKLIEEEVFLTAVDTASNDDIIRLQSIMDNMTEVPLAGGKPEDYHLLLDEYDLLMGR
IFGNQIYDKLMQTIIMLRKDFSHKLLKAEDAMAHSLKLRLKIHDAFVERDKAQLKKNLHTFFQSSRKFYDKMADN

Sequences:

>Translated_235_residues
MDVIALKKVTLTEQIMEQIASQITSGKLKPGQRLPNERAMGEMFNVTRSRVREALRALSLIGLIEIKPGDGSYVCENKPH
LPEETITWIFNKEIHNLDEVYAARKLIEEEVFLTAVDTASNDDIIRLQSIMDNMTEVPLAGGKPEDYHLLLDEYDLLMGR
IFGNQIYDKLMQTIIMLRKDFSHKLLKAEDAMAHSLKLRLKIHDAFVERDKAQLKKNLHTFFQSSRKFYDKMADN
>Mature_235_residues
MDVIALKKVTLTEQIMEQIASQITSGKLKPGQRLPNERAMGEMFNVTRSRVREALRALSLIGLIEIKPGDGSYVCENKPH
LPEETITWIFNKEIHNLDEVYAARKLIEEEVFLTAVDTASNDDIIRLQSIMDNMTEVPLAGGKPEDYHLLLDEYDLLMGR
IFGNQIYDKLMQTIIMLRKDFSHKLLKAEDAMAHSLKLRLKIHDAFVERDKAQLKKNLHTFFQSSRKFYDKMADN

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=143, Percent_Identity=30.0699300699301, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI48994961, Length=128, Percent_Identity=30.46875, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27080; Mature: 27080

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIALKKVTLTEQIMEQIASQITSGKLKPGQRLPNERAMGEMFNVTRSRVREALRALSL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGLIEIKPGDGSYVCENKPHLPEETITWIFNKEIHNLDEVYAARKLIEEEVFLTAVDTAS
HHEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
NDDIIRLQSIMDNMTEVPLAGGKPEDYHLLLDEYDLLMGRIFGNQIYDKLMQTIIMLRKD
CCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSHKLLKAEDAMAHSLKLRLKIHDAFVERDKAQLKKNLHTFFQSSRKFYDKMADN
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVIALKKVTLTEQIMEQIASQITSGKLKPGQRLPNERAMGEMFNVTRSRVREALRALSL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGLIEIKPGDGSYVCENKPHLPEETITWIFNKEIHNLDEVYAARKLIEEEVFLTAVDTAS
HHEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
NDDIIRLQSIMDNMTEVPLAGGKPEDYHLLLDEYDLLMGRIFGNQIYDKLMQTIIMLRKD
CCCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSHKLLKAEDAMAHSLKLRLKIHDAFVERDKAQLKKNLHTFFQSSRKFYDKMADN
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]