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The map label for this gene is purD [H]

Identifier: 85058120

GI number: 85058120

Start: 233755

End: 235038

Strand: Reverse

Name: purD [H]

Synonym: SG0142

Alternate gene names: 85058120

Gene position: 235038-233755 (Counterclockwise)

Preceding gene: 85058121

Following gene: 85058115

Centisome position: 5.63

GC content: 60.51

Gene sequence:

>1284_bases
ATGAATATTTTAATTATCGGCAACGGTGGACGTGAACACTCCTTGGCCTGGAAAGCTGCTCAGTCGACGCTTGCCGACAA
CGTCTATGTCGCGCCCGGCAATGCCGGCACCGCGCTGGAGCCGGCGCTGCAAAATGTGGATATCGCCGCCACCGATATTC
CGGCGCTGGTAGCCTTCGCCCGGGAGCAACACATCGGGCTGACCATTGTCGGTCCTGAAGCGCCGCTGGTGCAGGGCGTG
GTGGATGCCTTCCGCGCCGCCGATCTGAAGATTTTTGGCCCGACGCAGGCCGCCGCGCAGTTGGAAGGCTCCAAAGCTTT
CAGCAAAGACTTTCTCGCCCGCCACGGTATACCGACCGCACGCTATGAAAACTTTACCGAGGTTGCGCCGGCGCTGGACT
ATGTGCGTCAGCAAGGGGCGCCTATCGTCATCAAAGCGGACGGCCTGGCCGCCGGTAAAGGCGTAATCGTCGCCATGACG
CAGGTGGAAGCGGAATCCGCCGTAAACGATATGTTGGCAGGTAACGCTTTTGGCGATGCCGGGCATCGTATTGTCATCGA
GGAATTTCTCGATGGCGAAGAGGCCAGCTTTATTGTCATGGTGGACGGTGAGCATGTGTTGCCAATGGCCACCAGCCAAG
ATCATAAACGCGTCGGCGATGGGGATACCGGTCCGAACACCGGCGGCATGGGCGCCTATTCGCCGGCGCCGGTAGTTACC
GACGAGGTGCACCGCCGGGTGATGGAGCAGGTCATTTGGCCAACGGTAAAGGGGATGGCGGCTGAAGGCAATGTCTACAC
CGGCTTCCTGTATGCCGGTTTGATGATAAGCCCTGATGGTCAGCCGAAAGTTATTGAGTTCAACTGCCGCTTTGGCGATC
CGGAAACCCAACCTATTATGCTGCGCATGCGCTCTGATCTGGTCGCCCACTGCCTGGCGGCCACCGAAGGCCGTTTGAAC
GAGGAAAGGTCCGAATGGGATGAACGGCCGGCGCTGGGTGTGGTATTGGCGGCGGGAGGCTATCCCGGCGACTACCTCAC
TGGGGATGAAATCAGCGGTCTACCGGCAACCGAGGCGGAAGGAGAAAAAATCTTTCACGCCGGTACCCGCCTGCATCAGG
GCAAGGTACTGACCCAGGGAGGTCGCGTGCTATGCATCACCGCGCTGGGAGCCACTGTTGCCGATGCGCAGGCGAAGGCC
TACCGCGCGGCCCAGGCCGTCCAATGGCGGGAAAGCTTCTGCCGCCGCGATATCGGCTACCGCGCTATAGCCCGCGACGT
CTGA

Upstream 100 bases:

>100_bases
CACGATAATGAAATCATCGCCGCCGCCAATGAGCATGGCATTGCCATGATCTTCACCCATATGCGTCATTTCCGCCATTA
AGCCCAGGAGTTAGCTCGCC

Downstream 100 bases:

>100_bases
TACCGCTGCCAGCGGCAGAAATCCTCGTTGGCGACTAGCAATAATTGGGTACCTTCCGGTGCTTCAAGCCAAGCAATTCC
CAATGCGCCCGACGTATGAC

Product: phosphoribosylglycineamide synthase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNILIIGNGGREHSLAWKAAQSTLADNVYVAPGNAGTALEPALQNVDIAATDIPALVAFAREQHIGLTIVGPEAPLVQGV
VDAFRAADLKIFGPTQAAAQLEGSKAFSKDFLARHGIPTARYENFTEVAPALDYVRQQGAPIVIKADGLAAGKGVIVAMT
QVEAESAVNDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DEVHRRVMEQVIWPTVKGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIMLRMRSDLVAHCLAATEGRLN
EERSEWDERPALGVVLAAGGYPGDYLTGDEISGLPATEAEGEKIFHAGTRLHQGKVLTQGGRVLCITALGATVADAQAKA
YRAAQAVQWRESFCRRDIGYRAIARDV

Sequences:

>Translated_427_residues
MNILIIGNGGREHSLAWKAAQSTLADNVYVAPGNAGTALEPALQNVDIAATDIPALVAFAREQHIGLTIVGPEAPLVQGV
VDAFRAADLKIFGPTQAAAQLEGSKAFSKDFLARHGIPTARYENFTEVAPALDYVRQQGAPIVIKADGLAAGKGVIVAMT
QVEAESAVNDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DEVHRRVMEQVIWPTVKGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIMLRMRSDLVAHCLAATEGRLN
EERSEWDERPALGVVLAAGGYPGDYLTGDEISGLPATEAEGEKIFHAGTRLHQGKVLTQGGRVLCITALGATVADAQAKA
YRAAQAVQWRESFCRRDIGYRAIARDV
>Mature_427_residues
MNILIIGNGGREHSLAWKAAQSTLADNVYVAPGNAGTALEPALQNVDIAATDIPALVAFAREQHIGLTIVGPEAPLVQGV
VDAFRAADLKIFGPTQAAAQLEGSKAFSKDFLARHGIPTARYENFTEVAPALDYVRQQGAPIVIKADGLAAGKGVIVAMT
QVEAESAVNDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DEVHRRVMEQVIWPTVKGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIMLRMRSDLVAHCLAATEGRLN
EERSEWDERPALGVVLAAGGYPGDYLTGDEISGLPATEAEGEKIFHAGTRLHQGKVLTQGGRVLCITALGATVADAQAKA
YRAAQAVQWRESFCRRDIGYRAIARDV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50.2358490566038, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50.2358490566038, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50.2358490566038, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50.2358490566038, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=77.5175644028103, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.7452830188679, Blast_Score=310, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.5968109339408, Blast_Score=336, Evalue=4e-93,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=43.5897435897436, Blast_Score=324, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=43.5897435897436, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45373; Mature: 45373

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHSLAWKAAQSTLADNVYVAPGNAGTALEPALQNVDIAATDIPALVAFA
CEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCEECCCHHHHHHHH
REQHIGLTIVGPEAPLVQGVVDAFRAADLKIFGPTQAAAQLEGSKAFSKDFLARHGIPTA
HHCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHH
RYENFTEVAPALDYVRQQGAPIVIKADGLAAGKGVIVAMTQVEAESAVNDMLAGNAFGDA
HHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEVHRRVMEQVIWPTVKGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEECEEECCCCCCCEEEEEECCCCCCCCCEE
LRMRSDLVAHCLAATEGRLNEERSEWDERPALGVVLAAGGYPGDYLTGDEISGLPATEAE
EEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GEKIFHAGTRLHQGKVLTQGGRVLCITALGATVADAQAKAYRAAQAVQWRESFCRRDIGY
CCEEEECCCEEECCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RAIARDV
HHHHCCC
>Mature Secondary Structure
MNILIIGNGGREHSLAWKAAQSTLADNVYVAPGNAGTALEPALQNVDIAATDIPALVAFA
CEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCCEECCCHHHHHHHH
REQHIGLTIVGPEAPLVQGVVDAFRAADLKIFGPTQAAAQLEGSKAFSKDFLARHGIPTA
HHCCCCEEEECCCCHHHHHHHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCHH
RYENFTEVAPALDYVRQQGAPIVIKADGLAAGKGVIVAMTQVEAESAVNDMLAGNAFGDA
HHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCC
GHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
DEVHRRVMEQVIWPTVKGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEECEEECCCCCCCEEEEEECCCCCCCCCEE
LRMRSDLVAHCLAATEGRLNEERSEWDERPALGVVLAAGGYPGDYLTGDEISGLPATEAE
EEHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCC
GEKIFHAGTRLHQGKVLTQGGRVLCITALGATVADAQAKAYRAAQAVQWRESFCRRDIGY
CCEEEECCCEEECCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RAIARDV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]