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The map label for this gene is metR [H]

Identifier: 85058069

GI number: 85058069

Start: 140708

End: 141619

Strand: Reverse

Name: metR [H]

Synonym: SG0091

Alternate gene names: 85058069

Gene position: 141619-140708 (Counterclockwise)

Preceding gene: 85058093

Following gene: 85058066

Centisome position: 3.4

GC content: 59.65

Gene sequence:

>912_bases
ATGATCGAACTCAAACATCTGCGAACGCTGCAGGCGTTGCGTAACAGCGGTTCGCTGGCGGCGGCCGCATCGCAGCTACA
CCAGACCCAATCGGCGCTGTCGCATCAGTTCAGCGATCTGGAACAGCGGCTCGGCTTTCGCCTGTTCGTACGCAAAAGCC
AGCCGCTGCGTTTTACGCCGCAGGGCGAGATCCTGCTGCAGCTGGCCGAACAAATCTTGCCGCAGGTTCAGCAGGCTCTG
CAAACCTGCCAGCAACCGCACCAGGCCACGCTGCGTTTAGCTATCGAGTGCCATAGTTGTATACAGTGGATGACGCCGGC
CCTGGCGGAATTCCACCAACAATGGCCGCAAGTGTTAATGGATTTCCGCTCCGGCGTCACCTTCGATCCGCAACCGGCGC
TCCAGCAGGGCGAACTGGATTTGGTTTTGACCTCGGATATTCTGCCGCGTAGCGGGCTGTACTATGCGCCCATGTTCGAT
TTCGAGGTCCGGCTGGTGCTGTCGCCGGATCATCCGCTGGCGGCCAAAAAGGTCATTGCGCCGCAGGACCTGGACCAGGA
AACATTGCTTATCTATCCGGTACAGCGCAACCGCCTGGACATTTGGCGCCATTTCCTACAGCCGGCGGGCATTAATCCCA
TGTTGAAAACCGTGGATAACACCCTGCTGCTGATACAAATGGTATCGGCGCGCATGGGTATCGCCGCGTTGCCGCATTGG
GTCGTGGAAAGCTTTGAACGCCAGGGGCTTATCGCCACCCGGACGCTTGGGGAAGGGTTGTGGAGCCGGCTTTACGCTGC
CGTACGCGACGGCGAACAACGCCAGCCGGCGACCGAGGCATTCATCCACTCAGCCCAACAGCACGCCTGCGCGCATTTGC
CATATGTCCGCTGCGCCGAGGCAAACCTCTAA

Upstream 100 bases:

>100_bases
CCCTCTCCCATTTAGACGTCCAGATGTTTACACATCCATAATCACCATGTAGTGTACTGTTCACAAGCGCAATTTATTCA
CTTCCAGGTGAAACACTTTC

Downstream 100 bases:

>100_bases
CCTTGTATCTTGAAATTTAGTTAGCATGGTGACGAACGTTCCCGCAGGCAGACGGCGGCGCCCCAAAGCTCAGCTGCGTA
AACCGACCAACCGCTTTGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIELKHLRTLQALRNSGSLAAAASQLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLAEQILPQVQQAL
QTCQQPHQATLRLAIECHSCIQWMTPALAEFHQQWPQVLMDFRSGVTFDPQPALQQGELDLVLTSDILPRSGLYYAPMFD
FEVRLVLSPDHPLAAKKVIAPQDLDQETLLIYPVQRNRLDIWRHFLQPAGINPMLKTVDNTLLLIQMVSARMGIAALPHW
VVESFERQGLIATRTLGEGLWSRLYAAVRDGEQRQPATEAFIHSAQQHACAHLPYVRCAEANL

Sequences:

>Translated_303_residues
MIELKHLRTLQALRNSGSLAAAASQLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLAEQILPQVQQAL
QTCQQPHQATLRLAIECHSCIQWMTPALAEFHQQWPQVLMDFRSGVTFDPQPALQQGELDLVLTSDILPRSGLYYAPMFD
FEVRLVLSPDHPLAAKKVIAPQDLDQETLLIYPVQRNRLDIWRHFLQPAGINPMLKTVDNTLLLIQMVSARMGIAALPHW
VVESFERQGLIATRTLGEGLWSRLYAAVRDGEQRQPATEAFIHSAQQHACAHLPYVRCAEANL
>Mature_303_residues
MIELKHLRTLQALRNSGSLAAAASQLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEILLQLAEQILPQVQQAL
QTCQQPHQATLRLAIECHSCIQWMTPALAEFHQQWPQVLMDFRSGVTFDPQPALQQGELDLVLTSDILPRSGLYYAPMFD
FEVRLVLSPDHPLAAKKVIAPQDLDQETLLIYPVQRNRLDIWRHFLQPAGINPMLKTVDNTLLLIQMVSARMGIAALPHW
VVESFERQGLIATRTLGEGLWSRLYAAVRDGEQRQPATEAFIHSAQQHACAHLPYVRCAEANL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=300, Percent_Identity=82.6666666666667, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=27.2108843537415, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=25.2631578947368, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=254, Percent_Identity=27.9527559055118, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787530, Length=303, Percent_Identity=22.7722772277228, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=288, Percent_Identity=23.2638888888889, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081904, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786448, Length=214, Percent_Identity=28.9719626168224, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=234, Percent_Identity=26.0683760683761, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789506, Length=212, Percent_Identity=25.4716981132075, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=24.6212121212121, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=170, Percent_Identity=27.6470588235294, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=28.1746031746032, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=210, Percent_Identity=25.7142857142857, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=240, Percent_Identity=22.9166666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.0588235294118, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=25.130890052356, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI2367136, Length=238, Percent_Identity=23.5294117647059, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34326; Mature: 34326

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLRTLQALRNSGSLAAAASQLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLAEQILPQVQQALQTCQQPHQATLRLAIECHSCIQWMTPALAEFHQQWPQVLM
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFRSGVTFDPQPALQQGELDLVLTSDILPRSGLYYAPMFDFEVRLVLSPDHPLAAKKVIA
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHCCC
PQDLDQETLLIYPVQRNRLDIWRHFLQPAGINPMLKTVDNTLLLIQMVSARMGIAALPHW
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLIATRTLGEGLWSRLYAAVRDGEQRQPATEAFIHSAQQHACAHLPYVRCAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC
ANL
CCC
>Mature Secondary Structure
MIELKHLRTLQALRNSGSLAAAASQLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEILLQLAEQILPQVQQALQTCQQPHQATLRLAIECHSCIQWMTPALAEFHQQWPQVLM
CHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFRSGVTFDPQPALQQGELDLVLTSDILPRSGLYYAPMFDFEVRLVLSPDHPLAAKKVIA
HHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHCCC
PQDLDQETLLIYPVQRNRLDIWRHFLQPAGINPMLKTVDNTLLLIQMVSARMGIAALPHW
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVESFERQGLIATRTLGEGLWSRLYAAVRDGEQRQPATEAFIHSAQQHACAHLPYVRCAE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCC
ANL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]