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The map label for this gene is rfaH

Identifier: 85058093

GI number: 85058093

Start: 192914

End: 193405

Strand: Reverse

Name: rfaH

Synonym: SG0115

Alternate gene names: 85058093

Gene position: 193405-192914 (Counterclockwise)

Preceding gene: 85058104

Following gene: 85058069

Centisome position: 4.64

GC content: 50.61

Gene sequence:

>492_bases
ATGGAAGCTTGGTATTTATTGTATTGTAAACGCGGGCAATTACTGCGAGCTAAAGAACATCTGGAACGGCAGGCCGTTGC
CTGTTTGACGCCGATGGTCACCCTGGAGAAAATCATCCGGGGCAAGCGCACAGAAACCTGCGAGCCACTGTTTCCAAACT
ATCTGTTTATCGAGTTCGATCCGGAAACGATCCATACGACCACCATCAGCGCAACCCGCGGTGTAAGCCACTTTGTCCGC
TTCGGCAGCTTACCGGTAATTATCCCTTCCGCAGTCATTAACGATCTGCGCGAGCATAGTTTTGACAACGTGACCGATCC
TGAGACGCCTTTATCCGGCGATAAGGTATTGATCACCACCGGAGTATTTGAGGGGCTGCAGGCCATTTACACCGAACCCG
ACGGCGAGGCGCGCTCTATGCTGTTGCTGAATCTATTGAACAAACAGGTCTATCAAAGCCTGGATAATCGTCTGTTTCAG
AAAATGCAGTAG

Upstream 100 bases:

>100_bases
TAAGCCGTCCTGACTGCCCGACAGCGGCGGCGTCCTGTGCTATATATATGTTATGGCTTTTGCTATGCTGAGACTTCGAT
GAGATAGGTAAATTGTTACT

Downstream 100 bases:

>100_bases
CCCGTATTAGCGCGGCGGACAACGCCGAAAAATCTGGCCATAGCAGCTACCTACAGCCCAAACCGCCTTAGTCCTGACAG
ACGTCATCGTGGCGCATACC

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MEAWYLLYCKRGQLLRAKEHLERQAVACLTPMVTLEKIIRGKRTETCEPLFPNYLFIEFDPETIHTTTISATRGVSHFVR
FGSLPVIIPSAVINDLREHSFDNVTDPETPLSGDKVLITTGVFEGLQAIYTEPDGEARSMLLLNLLNKQVYQSLDNRLFQ
KMQ

Sequences:

>Translated_163_residues
MEAWYLLYCKRGQLLRAKEHLERQAVACLTPMVTLEKIIRGKRTETCEPLFPNYLFIEFDPETIHTTTISATRGVSHFVR
FGSLPVIIPSAVINDLREHSFDNVTDPETPLSGDKVLITTGVFEGLQAIYTEPDGEARSMLLLNLLNKQVYQSLDNRLFQ
KMQ
>Mature_163_residues
MEAWYLLYCKRGQLLRAKEHLERQAVACLTPMVTLEKIIRGKRTETCEPLFPNYLFIEFDPETIHTTTISATRGVSHFVR
FGSLPVIIPSAVINDLREHSFDNVTDPETPLSGDKVLITTGVFEGLQAIYTEPDGEARSMLLLNLLNKQVYQSLDNRLFQ
KMQ

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=162, Percent_Identity=70.3703703703704, Blast_Score=252, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 18616; Mature: 18616

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAWYLLYCKRGQLLRAKEHLERQAVACLTPMVTLEKIIRGKRTETCEPLFPNYLFIEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
PETIHTTTISATRGVSHFVRFGSLPVIIPSAVINDLREHSFDNVTDPETPLSGDKVLITT
CCEEEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEH
GVFEGLQAIYTEPDGEARSMLLLNLLNKQVYQSLDNRLFQKMQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEAWYLLYCKRGQLLRAKEHLERQAVACLTPMVTLEKIIRGKRTETCEPLFPNYLFIEFD
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
PETIHTTTISATRGVSHFVRFGSLPVIIPSAVINDLREHSFDNVTDPETPLSGDKVLITT
CCEEEEEECHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEH
GVFEGLQAIYTEPDGEARSMLLLNLLNKQVYQSLDNRLFQKMQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]