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The map label for this gene is folE [H]

Identifier: 84625993

GI number: 84625993

Start: 4901874

End: 4902503

Strand: Reverse

Name: folE [H]

Synonym: XOO_4336

Alternate gene names: 84625993

Gene position: 4902503-4901874 (Counterclockwise)

Preceding gene: 84625997

Following gene: 84625992

Centisome position: 99.24

GC content: 63.81

Gene sequence:

>630_bases
ATGCTCGCCCCCTTTTGGTGCACCTGCATGAGCCAGTCCGACCAGCCCGATTCCTCCGTGACCCAAACCCAGGCCGAAGA
CGCCGTGCGCATCTTGTTGCGCTGGGCGGGCGAAGATCCGACGCGTGAAGGCCTGCTGGACACCCCACGCCGGGTGGCCG
AAGCCTATGGCGACTGGTTCAGCGGCTACCGCATAGAACCGCGCGAATACCTGGAACGCACCTTCGAAGAAGTGGCCGGC
TATGACGAACTGATCGTGCTGCGCGACATCTCGTATGAAAGCCATTGCGAGCACCACATGGCGCCGATCATCGGCAAGGT
GCACGTGGGCTATCTGCCCCGCTGCAAGGTGGTCGGCATCAGCAAGCTGGCCCGCGTGGTGGAAAGCTACGCGCGCCGCT
TCCAGGTGCAGGAAAAGATGACCGCACAGATCGCCCAGTGCATCCAGGACGTGCTGCAACCGCTCGGCGTGGGCGTGGTG
GTGGAAGGCGCGCACGAATGCATGACCACCCGCGGCATCCACAAGCGCGGCGTCAGCATGGTGACCTCCAAGATGCTGGG
CAGCTTCCGCGAAGACGCGCGCACCCGCGCCGAATTCCTGCAGTTCATCGAAGTGGGTGGCAAGCGTTGA

Upstream 100 bases:

>100_bases
AACAGATAGGATGCTATCAATTCACTAATGAATCAGTAGTCGCTGAACATTTGCAAACTGGCGCAACCGACGTGGGGCAG
CCGCTCAGTCCTCGGGTACC

Downstream 100 bases:

>100_bases
GGTCGAGCTTGGCGTTGATGGCTGGTAGATTGATGCTCTACAAGCTGGCGGGACACCGTCGCTAAGGAATCGCATGCAGA
TTGAATCTATCGAAATCAAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MLAPFWCTCMSQSDQPDSSVTQTQAEDAVRILLRWAGEDPTREGLLDTPRRVAEAYGDWFSGYRIEPREYLERTFEEVAG
YDELIVLRDISYESHCEHHMAPIIGKVHVGYLPRCKVVGISKLARVVESYARRFQVQEKMTAQIAQCIQDVLQPLGVGVV
VEGAHECMTTRGIHKRGVSMVTSKMLGSFREDARTRAEFLQFIEVGGKR

Sequences:

>Translated_209_residues
MLAPFWCTCMSQSDQPDSSVTQTQAEDAVRILLRWAGEDPTREGLLDTPRRVAEAYGDWFSGYRIEPREYLERTFEEVAG
YDELIVLRDISYESHCEHHMAPIIGKVHVGYLPRCKVVGISKLARVVESYARRFQVQEKMTAQIAQCIQDVLQPLGVGVV
VEGAHECMTTRGIHKRGVSMVTSKMLGSFREDARTRAEFLQFIEVGGKR
>Mature_209_residues
MLAPFWCTCMSQSDQPDSSVTQTQAEDAVRILLRWAGEDPTREGLLDTPRRVAEAYGDWFSGYRIEPREYLERTFEEVAG
YDELIVLRDISYESHCEHHMAPIIGKVHVGYLPRCKVVGISKLARVVESYARRFQVQEKMTAQIAQCIQDVLQPLGVGVV
VEGAHECMTTRGIHKRGVSMVTSKMLGSFREDARTRAEFLQFIEVGGKR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=188, Percent_Identity=47.8723404255319, Blast_Score=177, Evalue=5e-45,
Organism=Homo sapiens, GI4503949, Length=188, Percent_Identity=47.8723404255319, Blast_Score=177, Evalue=5e-45,
Organism=Homo sapiens, GI66932970, Length=158, Percent_Identity=43.6708860759494, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI66932972, Length=148, Percent_Identity=45.945945945946, Blast_Score=131, Evalue=3e-31,
Organism=Escherichia coli, GI1788476, Length=175, Percent_Identity=36, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=179, Percent_Identity=47.4860335195531, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=51.123595505618, Blast_Score=181, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=48.2954545454545, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=48.2954545454545, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=48.2954545454545, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23712; Mature: 23712

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAPFWCTCMSQSDQPDSSVTQTQAEDAVRILLRWAGEDPTREGLLDTPRRVAEAYGDWF
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SGYRIEPREYLERTFEEVAGYDELIVLRDISYESHCEHHMAPIIGKVHVGYLPRCKVVGI
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SKLARVVESYARRFQVQEKMTAQIAQCIQDVLQPLGVGVVVEGAHECMTTRGIHKRGVSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHH
VTSKMLGSFREDARTRAEFLQFIEVGGKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAPFWCTCMSQSDQPDSSVTQTQAEDAVRILLRWAGEDPTREGLLDTPRRVAEAYGDWF
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
SGYRIEPREYLERTFEEVAGYDELIVLRDISYESHCEHHMAPIIGKVHVGYLPRCKVVGI
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
SKLARVVESYARRFQVQEKMTAQIAQCIQDVLQPLGVGVVVEGAHECMTTRGIHKRGVSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHH
VTSKMLGSFREDARTRAEFLQFIEVGGKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]