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The map label for this gene is queF [H]

Identifier: 84625611

GI number: 84625611

Start: 4453703

End: 4454518

Strand: Reverse

Name: queF [H]

Synonym: XOO_3954

Alternate gene names: 84625611

Gene position: 4454518-4453703 (Counterclockwise)

Preceding gene: 84625613

Following gene: 84625610

Centisome position: 90.17

GC content: 64.09

Gene sequence:

>816_bases
ATGAATACCCCAGAAGATTCCACTCTTGGTCGCGAGGTCGCCTATCCGAGCGGCTACGACCCGTCGTTGCTGTTTCCGAT
TCCACGTGCTGCCGGACGCGAGGCGATCGGGCTGACCGGCGCCTTGCCGTTTACCGGCCGCGATCGCTGGCATGCCTACG
AGCTCAGTTGGCTCGACGCACACGGCAAGCCCTGCGTGGCCACCGCCACCTTGCATGTGCCACATGACTCGCCGGCGTTG
ATCGAATCCAAGTCGCTCAAGCTGTATCTCAACTCGCTCAACGCGACCCGTTTCAACAGCGCCGAAGCGGTGCGTACGCG
CATCGTCACCGACCTGTCCACGCGTGCCGGTGCCGATGTCTCGGTGGAGTTCGGCCTGCCGCCGATCGATGGGGTGGGCG
ATGGCGAATCGATCGACGCGCTGGATGTCAGCATCGACGATTACGGCCCACCCAACGCCGCGTATCTGACGGCGCAGGCA
CAACCAGTGGTGGAAGAAGTGCTGACCTCGGCCCTGCTCAAGTCCAATTGCCCGGTCACCGGTCAACCGGATTGGGCCAG
CGTCACCCTGCATTACCGCGGCGCACCGATCGAGCGCGAAGGGCTGCTGCGCTATCTGGTGAGTTTCCGCGACCACGCCG
AATTTCATGAGCAATGCGTGGAGCGTATCTTCCATGACGTGCTGCTCCGCTGTGCGCCCGAATGGCTGGTGGTGGAAGCG
CGCTACACCCGCCGTGGCGGGCTGGACATCAATCCGCTGCGCAGCTCGCTCAGCGTGCCGGCACCGCTGTCGGTCTTCCG
CGACCTGCGCCAGTAA

Upstream 100 bases:

>100_bases
GCGCCGGCGCCCTCGCGCTCCCATGCTCCCGTCTGTCAATCGCGTAAGCCCGTGGGCGTCATACACCTCACGGCCAGCCC
GGGCGGCCTAGAATAGCCGG

Downstream 100 bases:

>100_bases
TCGCATCATTCAAGGGTCGCGCCGGCTTCACGGCGGCACGATCCATGCTTGACGGCACTCTGTGCAGCCTGCAGGTCCGG
TGATGCAATGCAGGGCTGCA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGREAIGLTGALPFTGRDRWHAYELSWLDAHGKPCVATATLHVPHDSPAL
IESKSLKLYLNSLNATRFNSAEAVRTRIVTDLSTRAGADVSVEFGLPPIDGVGDGESIDALDVSIDDYGPPNAAYLTAQA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIEREGLLRYLVSFRDHAEFHEQCVERIFHDVLLRCAPEWLVVEA
RYTRRGGLDINPLRSSLSVPAPLSVFRDLRQ

Sequences:

>Translated_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGREAIGLTGALPFTGRDRWHAYELSWLDAHGKPCVATATLHVPHDSPAL
IESKSLKLYLNSLNATRFNSAEAVRTRIVTDLSTRAGADVSVEFGLPPIDGVGDGESIDALDVSIDDYGPPNAAYLTAQA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIEREGLLRYLVSFRDHAEFHEQCVERIFHDVLLRCAPEWLVVEA
RYTRRGGLDINPLRSSLSVPAPLSVFRDLRQ
>Mature_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGREAIGLTGALPFTGRDRWHAYELSWLDAHGKPCVATATLHVPHDSPAL
IESKSLKLYLNSLNATRFNSAEAVRTRIVTDLSTRAGADVSVEFGLPPIDGVGDGESIDALDVSIDDYGPPNAAYLTAQA
QPVVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIEREGLLRYLVSFRDHAEFHEQCVERIFHDVLLRCAPEWLVVEA
RYTRRGGLDINPLRSSLSVPAPLSVFRDLRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=268, Percent_Identity=52.2388059701493, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 29714; Mature: 29714

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGREAIGLTGALPFTGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCEEEEEEEEECC
HGKPCVATATLHVPHDSPALIESKSLKLYLNSLNATRFNSAEAVRTRIVTDLSTRAGADV
CCCCEEEEEEEECCCCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCE
SVEFGLPPIDGVGDGESIDALDVSIDDYGPPNAAYLTAQAQPVVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLHYRGAPIEREGLLRYLVSFRDHAEFHEQCVERIFHDVLLRCAPEWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RYTRRGGLDINPLRSSLSVPAPLSVFRDLRQ
EECCCCCCCCHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGREAIGLTGALPFTGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCEEEEEEEEECC
HGKPCVATATLHVPHDSPALIESKSLKLYLNSLNATRFNSAEAVRTRIVTDLSTRAGADV
CCCCEEEEEEEECCCCCCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCE
SVEFGLPPIDGVGDGESIDALDVSIDDYGPPNAAYLTAQAQPVVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLHYRGAPIEREGLLRYLVSFRDHAEFHEQCVERIFHDVLLRCAPEWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RYTRRGGLDINPLRSSLSVPAPLSVFRDLRQ
EECCCCCCCCHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]