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The map label for this gene is oprF [H]

Identifier: 84625147

GI number: 84625147

Start: 3954855

End: 3955952

Strand: Reverse

Name: oprF [H]

Synonym: XOO_3490

Alternate gene names: 84625147

Gene position: 3955952-3954855 (Counterclockwise)

Preceding gene: 84625160

Following gene: 84625146

Centisome position: 80.08

GC content: 62.39

Gene sequence:

>1098_bases
ATGAACAAGAAAATTCTCACTGCCGCGTTGCTTGGCGGTCTGGCAGTTGCCCAGGCTGCTTCCGCGCAGGAGTTCGACGA
CCGTTGGTACCTGACCGGTAGCGCTGGCTTCAATTTCCAGGACCGCGATCGTTTGACCAATGATGCCCCGTTCGTGACCC
TGGGCCTGGGCAAGTTCGTGAGCCCGAACTGGTCGATCGACGGTGAACTGAACTATCAGAACCCGAACTTCGACGCCAAC
CAGGATCTGAACTGGAGCCAGTACGGCATCTCGTTCGACCTGCGTCGCCACTTCATCCAGGAAGGCCGCGGCTGGAACCC
CTACCTGCTGTTCGGTCTGGGCTATCAGCGTTCGGAAGAAGAATTCGACGCGACCCCGAACCCGGTGTCGCCGGGCCAGC
GTAAGGACGGCAACTTTGCCGCCAAGGCTGGTGTCGGTCTGCAGACCACCTTCGACAAGCGCGTCGCCGTGCGTGCCGAG
TTGGCCTATCGCGCTGACTTCAACGACCAGAGCGTCGCCGCTCCGCGCGAAAACTGGTTCGGCGACGTGCTGGCTTCGGT
CGGCGTCGTGATCCCGTTGGGACCGGCTCCGTCGACCGCTCCGCCGCCGGCTCCGGCTCCGGTTGCTCCGAGCTGCGCCG
ATCTGGATGACGACGGTGACGGCGTCAACAACTGCGACGACAAGTGCCCGAACTCGCAGCCTGGCCAGACCATCGGTCCG
GACGGTTGCCCGGTGCCTGTCTCGATCGACCTGAAGGGCGTGAACTTCGACTTCAACAAGTCGACCCTGCGTCCGGACGC
GATCTCGATCCTGAGCGAAGCGACCGAAATCCTGAAGCGCTACCCCGACCTGAAGGTCGAGGTTGCCGGTCACACCGACT
CGAAGGGTACCGACGCGTACAACCAGAAGCTGTCCGAGCGTCGTGCGACCACCGTGTACGACTACCTGACCAAGAACGGC
GTCGATGCGTCGCGTCTGGTCGGTCCGATCGGCTACGGCAAGAGCCGTCCGATTGCTCCGAACACCAACCCGGATGGTTC
CGACAATCCGGAAGGCCGTGCGAAGAACCGTCGTACCGAGTTGAACGTCCAGAACTAA

Upstream 100 bases:

>100_bases
GCCGGCTTAATCTGCGCCCCGATGGCTCTGGATGTGACGAGCATCTTTACCAAGAACAATGTCTCTATCGGTTTTATCTC
CCAAAGAAGGAGCTGTATAA

Downstream 100 bases:

>100_bases
TTGGACCGCTAGCGTTGTTGCAGACAAAACCCGGCCTTGCGCCGGGTTTTGTCTATGCAGGCCCTGGAAAAGCGCGCTCA
AGCGCTGCTGATCCTGTCTG

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNKKILTAALLGGLAVAQAASAQEFDDRWYLTGSAGFNFQDRDRLTNDAPFVTLGLGKFVSPNWSIDGELNYQNPNFDAN
QDLNWSQYGISFDLRRHFIQEGRGWNPYLLFGLGYQRSEEEFDATPNPVSPGQRKDGNFAAKAGVGLQTTFDKRVAVRAE
LAYRADFNDQSVAAPRENWFGDVLASVGVVIPLGPAPSTAPPPAPAPVAPSCADLDDDGDGVNNCDDKCPNSQPGQTIGP
DGCPVPVSIDLKGVNFDFNKSTLRPDAISILSEATEILKRYPDLKVEVAGHTDSKGTDAYNQKLSERRATTVYDYLTKNG
VDASRLVGPIGYGKSRPIAPNTNPDGSDNPEGRAKNRRTELNVQN

Sequences:

>Translated_365_residues
MNKKILTAALLGGLAVAQAASAQEFDDRWYLTGSAGFNFQDRDRLTNDAPFVTLGLGKFVSPNWSIDGELNYQNPNFDAN
QDLNWSQYGISFDLRRHFIQEGRGWNPYLLFGLGYQRSEEEFDATPNPVSPGQRKDGNFAAKAGVGLQTTFDKRVAVRAE
LAYRADFNDQSVAAPRENWFGDVLASVGVVIPLGPAPSTAPPPAPAPVAPSCADLDDDGDGVNNCDDKCPNSQPGQTIGP
DGCPVPVSIDLKGVNFDFNKSTLRPDAISILSEATEILKRYPDLKVEVAGHTDSKGTDAYNQKLSERRATTVYDYLTKNG
VDASRLVGPIGYGKSRPIAPNTNPDGSDNPEGRAKNRRTELNVQN
>Mature_365_residues
MNKKILTAALLGGLAVAQAASAQEFDDRWYLTGSAGFNFQDRDRLTNDAPFVTLGLGKFVSPNWSIDGELNYQNPNFDAN
QDLNWSQYGISFDLRRHFIQEGRGWNPYLLFGLGYQRSEEEFDATPNPVSPGQRKDGNFAAKAGVGLQTTFDKRVAVRAE
LAYRADFNDQSVAAPRENWFGDVLASVGVVIPLGPAPSTAPPPAPAPVAPSCADLDDDGDGVNNCDDKCPNSQPGQTIGP
DGCPVPVSIDLKGVNFDFNKSTLRPDAISILSEATEILKRYPDLKVEVAGHTDSKGTDAYNQKLSERRATTVYDYLTKNG
VDASRLVGPIGYGKSRPIAPNTNPDGSDNPEGRAKNRRTELNVQN

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=110, Percent_Identity=40, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787191, Length=120, Percent_Identity=40, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786962, Length=108, Percent_Identity=37.962962962963, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 39585; Mature: 39585

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKILTAALLGGLAVAQAASAQEFDDRWYLTGSAGFNFQDRDRLTNDAPFVTLGLGKFV
CCCHHHHHHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCHHHCCCCCCCEEEEECCCCC
SPNWSIDGELNYQNPNFDANQDLNWSQYGISFDLRRHFIQEGRGWNPYLLFGLGYQRSEE
CCCCCCCCEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
EFDATPNPVSPGQRKDGNFAAKAGVGLQTTFDKRVAVRAELAYRADFNDQSVAAPRENWF
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHH
GDVLASVGVVIPLGPAPSTAPPPAPAPVAPSCADLDDDGDGVNNCDDKCPNSQPGQTIGP
HHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
DGCPVPVSIDLKGVNFDFNKSTLRPDAISILSEATEILKRYPDLKVEVAGHTDSKGTDAY
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
NQKLSERRATTVYDYLTKNGVDASRLVGPIGYGKSRPIAPNTNPDGSDNPEGRAKNRRTE
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LNVQN
ECCCC
>Mature Secondary Structure
MNKKILTAALLGGLAVAQAASAQEFDDRWYLTGSAGFNFQDRDRLTNDAPFVTLGLGKFV
CCCHHHHHHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCHHHCCCCCCCEEEEECCCCC
SPNWSIDGELNYQNPNFDANQDLNWSQYGISFDLRRHFIQEGRGWNPYLLFGLGYQRSEE
CCCCCCCCEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
EFDATPNPVSPGQRKDGNFAAKAGVGLQTTFDKRVAVRAELAYRADFNDQSVAAPRENWF
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHH
GDVLASVGVVIPLGPAPSTAPPPAPAPVAPSCADLDDDGDGVNNCDDKCPNSQPGQTIGP
HHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
DGCPVPVSIDLKGVNFDFNKSTLRPDAISILSEATEILKRYPDLKVEVAGHTDSKGTDAY
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
NQKLSERRATTVYDYLTKNGVDASRLVGPIGYGKSRPIAPNTNPDGSDNPEGRAKNRRTE
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
LNVQN
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]