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The map label for this gene is cysW [H]

Identifier: 84625140

GI number: 84625140

Start: 3948717

End: 3949670

Strand: Reverse

Name: cysW [H]

Synonym: XOO_3483

Alternate gene names: 84625140

Gene position: 3949670-3948717 (Counterclockwise)

Preceding gene: 84625141

Following gene: 84625139

Centisome position: 79.95

GC content: 63.1

Gene sequence:

>954_bases
ATGAGCGATGCTGTTTCCTCGCTGCCCTCCATGTTGCCGACCCATGCCATGACCAGGCAGGCGCGTCGCATCACCGAGTC
GGCCAGCAACGAGCCGCGTTGGGTGCAATGGCTGATGATGTTCGGCGCGGTACTCTTTTTGTTGGCGTTTTTGCTGCTGC
CGCTGGTGCTGGTGTTTGCCGAGGCGTTGCGTGGCGGTTGGGAGACCTTCCTGCGCGTAGTGGTCGACCCGGACGCGTTG
TCGGCGATCAAGCTGACCTTGCTGGTGACGGCGATCGTGCTGCCATTGAATCTGATATTCGGCGTGGCCGCTGCGTGGGC
CATCAGCAAGCACCAGTTCTGGGGCAAACGTCTGCTGGTCAGCCTGATCGACCTGCCGTTTTCGGTGTCGCCGGTGGTGG
CGGGCTTGATCTTCGTGCTGATCTTCGGCCGCAGCGGCTGGGCCTGGCCATTGATCGACGAAGGCTGGCATGTGCAGTTG
CCGGCGCTGGGCGAATGTGTGATCCAGCTGCCGCGGATCGTGTTCGCGTTGCCGGGCATCGTGCTGGCGACCACGTTCGT
GACCTTCCCCTTCATCGCGCGCGAACTGATGCCGCTGATGGAGCAGCAGGGCAGCGACGAAGAACTGGCGGCACTGAGTC
TGGGCGCGAACGGCTGGCAGATGTTCTGGCGAGTGACGCTGCCCAATATCCGTTGGGGCCTGTTGTATGGCGTGCTGCTG
TGTGCGGCGCGTGCGATGGGCGAATTCGGCGCTGTGTCGGTGGTGTCCGGGCATATCCGTGGACGCACCAACACGCTGCC
GCTGCATGTGGAAATTCTCTACAACGAATATGCCTACAGCGCCGCATTCGCCTGCGCCTCGCTGCTGGCACTGACCGCGC
TGCTGACCTTGGCGCTGAAGACGTATCTGGAATGGCGGCACGGCGATTCGCTGGCCGCCAACCACCGCCACTGA

Upstream 100 bases:

>100_bases
CGACTGCCATTGCCGCGGCGATGTTATTACTGTCATTCGTCATTTTGCTGGTGGTCAATACCTTGCAGGCACGCCTGCTG
CAGTATCAACGGAGGCCCGC

Downstream 100 bases:

>100_bases
GGTGACCATGGGCATCCGCATCCACCATCTGCGCAAGCAGTTCGAGACCTTTACCGCGCTGGACGACATCACCCTGGATG
TGCGCCAGGGCGAGTTGCTG

Product: ABC transporter sulfate permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSDAVSSLPSMLPTHAMTRQARRITESASNEPRWVQWLMMFGAVLFLLAFLLLPLVLVFAEALRGGWETFLRVVVDPDAL
SAIKLTLLVTAIVLPLNLIFGVAAAWAISKHQFWGKRLLVSLIDLPFSVSPVVAGLIFVLIFGRSGWAWPLIDEGWHVQL
PALGECVIQLPRIVFALPGIVLATTFVTFPFIARELMPLMEQQGSDEELAALSLGANGWQMFWRVTLPNIRWGLLYGVLL
CAARAMGEFGAVSVVSGHIRGRTNTLPLHVEILYNEYAYSAAFACASLLALTALLTLALKTYLEWRHGDSLAANHRH

Sequences:

>Translated_317_residues
MSDAVSSLPSMLPTHAMTRQARRITESASNEPRWVQWLMMFGAVLFLLAFLLLPLVLVFAEALRGGWETFLRVVVDPDAL
SAIKLTLLVTAIVLPLNLIFGVAAAWAISKHQFWGKRLLVSLIDLPFSVSPVVAGLIFVLIFGRSGWAWPLIDEGWHVQL
PALGECVIQLPRIVFALPGIVLATTFVTFPFIARELMPLMEQQGSDEELAALSLGANGWQMFWRVTLPNIRWGLLYGVLL
CAARAMGEFGAVSVVSGHIRGRTNTLPLHVEILYNEYAYSAAFACASLLALTALLTLALKTYLEWRHGDSLAANHRH
>Mature_316_residues
SDAVSSLPSMLPTHAMTRQARRITESASNEPRWVQWLMMFGAVLFLLAFLLLPLVLVFAEALRGGWETFLRVVVDPDALS
AIKLTLLVTAIVLPLNLIFGVAAAWAISKHQFWGKRLLVSLIDLPFSVSPVVAGLIFVLIFGRSGWAWPLIDEGWHVQLP
ALGECVIQLPRIVFALPGIVLATTFVTFPFIARELMPLMEQQGSDEELAALSLGANGWQMFWRVTLPNIRWGLLYGVLLC
AARAMGEFGAVSVVSGHIRGRTNTLPLHVEILYNEYAYSAAFACASLLALTALLTLALKTYLEWRHGDSLAANHRH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=304, Percent_Identity=46.3815789473684, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1788764, Length=243, Percent_Identity=32.9218106995885, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786980, Length=197, Percent_Identity=32.994923857868, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34985; Mature: 34854

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAVSSLPSMLPTHAMTRQARRITESASNEPRWVQWLMMFGAVLFLLAFLLLPLVLVFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALRGGWETFLRVVVDPDALSAIKLTLLVTAIVLPLNLIFGVAAAWAISKHQFWGKRLLV
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIDLPFSVSPVVAGLIFVLIFGRSGWAWPLIDEGWHVQLPALGECVIQLPRIVFALPGI
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
VLATTFVTFPFIARELMPLMEQQGSDEELAALSLGANGWQMFWRVTLPNIRWGLLYGVLL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHH
CAARAMGEFGAVSVVSGHIRGRTNTLPLHVEILYNEYAYSAAFACASLLALTALLTLALK
HHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLEWRHGDSLAANHRH
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDAVSSLPSMLPTHAMTRQARRITESASNEPRWVQWLMMFGAVLFLLAFLLLPLVLVFA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALRGGWETFLRVVVDPDALSAIKLTLLVTAIVLPLNLIFGVAAAWAISKHQFWGKRLLV
HHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLIDLPFSVSPVVAGLIFVLIFGRSGWAWPLIDEGWHVQLPALGECVIQLPRIVFALPGI
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
VLATTFVTFPFIARELMPLMEQQGSDEELAALSLGANGWQMFWRVTLPNIRWGLLYGVLL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHH
CAARAMGEFGAVSVVSGHIRGRTNTLPLHVEILYNEYAYSAAFACASLLALTALLTLALK
HHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYLEWRHGDSLAANHRH
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]