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The map label for this gene is cysU [H]

Identifier: 84625141

GI number: 84625141

Start: 3949667

End: 3950527

Strand: Reverse

Name: cysU [H]

Synonym: XOO_3484

Alternate gene names: 84625141

Gene position: 3950527-3949667 (Counterclockwise)

Preceding gene: 84625142

Following gene: 84625140

Centisome position: 79.97

GC content: 64.0

Gene sequence:

>861_bases
ATGGCGATGTCGGTTGCACCCCACTCAGCGCCGTCATCGCGCCGACGCGTGATGCCGGGGCTGGGCCTGAGTTTGGGTAT
CACCCTGACCTGGCTTGGCCTGATCGTGCTGATCCCGCTGCTGGGGATCTTTATCAAGACCAGCGGCCTGGGTTGGGAGG
GTCTGTGGCGGATATGGAGCGAGCCGCGCGTGCTGTCGGCATTGCGGGTGAGCTTCGGTACGGCGCTTGCGGCTGGCGCT
TTCAATGCGGTGATGGGCACGTGGGTGGCGTGGGTGTTCGTGCGCTACCAGTTTCCGGGCAAGCGCTTCCTGGATGCGGT
GATCGATCTGCCGTTTGCGTTGCCGACGGCGGTGGCCGGCATTGCGCTGACCGCGCTGTATGGCGGCAAGGGTTGGGTCG
GCCGCTGGCTGGAAGCAATCGGTATCAAGGTGGCTTACACGCAGCTGGGCATCGTGCTGGCGCTGGTGTTCGTCGGCTTA
CCGTTCGTGGTGCGCGTGGTGCAGCCGGTGCTGGCCGAGAGCGATCGCGAAGTAGAGGCTGCCGCTGCCACATTGGGCGC
CTCGCGCTGGCAAACGGTATGGCGGGTAGTGCTGCCAGGCGTGTGGCCAGCGGTCCTGACCGGATTCGCGCTGGCGTTCG
CACGTGGCGTGGGTGAGTACGGTTCGGTGATCTTCATCGCCGGCAACTTGCCCAATTCCACCGAGATCGCGCCGCTGCTG
ATCACCATCCGGCTGGAAGAGTTCGACTATGCGGGCGCGACTGCCATTGCCGCGGCGATGTTATTACTGTCATTCGTCAT
TTTGCTGGTGGTCAATACCTTGCAGGCACGCCTGCTGCAGTATCAACGGAGGCCCGCATGA

Upstream 100 bases:

>100_bases
GGTGACCATCCAGCAGGCGTTTGGCTCTTGGGACAAGGCGCAACAGGAGCACTTCGCCGACGGTGGTGTGTTCGACCAGA
TCCAGGCCAACAAGTAGGCG

Downstream 100 bases:

>100_bases
GCGATGCTGTTTCCTCGCTGCCCTCCATGTTGCCGACCCATGCCATGACCAGGCAGGCGCGTCGCATCACCGAGTCGGCC
AGCAACGAGCCGCGTTGGGT

Product: ABC transporter sulfate permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MAMSVAPHSAPSSRRRVMPGLGLSLGITLTWLGLIVLIPLLGIFIKTSGLGWEGLWRIWSEPRVLSALRVSFGTALAAGA
FNAVMGTWVAWVFVRYQFPGKRFLDAVIDLPFALPTAVAGIALTALYGGKGWVGRWLEAIGIKVAYTQLGIVLALVFVGL
PFVVRVVQPVLAESDREVEAAAATLGASRWQTVWRVVLPGVWPAVLTGFALAFARGVGEYGSVIFIAGNLPNSTEIAPLL
ITIRLEEFDYAGATAIAAAMLLLSFVILLVVNTLQARLLQYQRRPA

Sequences:

>Translated_286_residues
MAMSVAPHSAPSSRRRVMPGLGLSLGITLTWLGLIVLIPLLGIFIKTSGLGWEGLWRIWSEPRVLSALRVSFGTALAAGA
FNAVMGTWVAWVFVRYQFPGKRFLDAVIDLPFALPTAVAGIALTALYGGKGWVGRWLEAIGIKVAYTQLGIVLALVFVGL
PFVVRVVQPVLAESDREVEAAAATLGASRWQTVWRVVLPGVWPAVLTGFALAFARGVGEYGSVIFIAGNLPNSTEIAPLL
ITIRLEEFDYAGATAIAAAMLLLSFVILLVVNTLQARLLQYQRRPA
>Mature_285_residues
AMSVAPHSAPSSRRRVMPGLGLSLGITLTWLGLIVLIPLLGIFIKTSGLGWEGLWRIWSEPRVLSALRVSFGTALAAGAF
NAVMGTWVAWVFVRYQFPGKRFLDAVIDLPFALPTAVAGIALTALYGGKGWVGRWLEAIGIKVAYTQLGIVLALVFVGLP
FVVRVVQPVLAESDREVEAAAATLGASRWQTVWRVVLPGVWPAVLTGFALAFARGVGEYGSVIFIAGNLPNSTEIAPLLI
TIRLEEFDYAGATAIAAAMLLLSFVILLVVNTLQARLLQYQRRPA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=266, Percent_Identity=49.2481203007519, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI87082099, Length=260, Percent_Identity=33.0769230769231, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1786980, Length=159, Percent_Identity=38.9937106918239, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787368, Length=234, Percent_Identity=27.3504273504274, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786254, Length=224, Percent_Identity=26.7857142857143, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30739; Mature: 30608

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSVAPHSAPSSRRRVMPGLGLSLGITLTWLGLIVLIPLLGIFIKTSGLGWEGLWRIWS
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
EPRVLSALRVSFGTALAAGAFNAVMGTWVAWVFVRYQFPGKRFLDAVIDLPFALPTAVAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
IALTALYGGKGWVGRWLEAIGIKVAYTQLGIVLALVFVGLPFVVRVVQPVLAESDREVEA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AAATLGASRWQTVWRVVLPGVWPAVLTGFALAFARGVGEYGSVIFIAGNLPNSTEIAPLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
ITIRLEEFDYAGATAIAAAMLLLSFVILLVVNTLQARLLQYQRRPA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AMSVAPHSAPSSRRRVMPGLGLSLGITLTWLGLIVLIPLLGIFIKTSGLGWEGLWRIWS
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
EPRVLSALRVSFGTALAAGAFNAVMGTWVAWVFVRYQFPGKRFLDAVIDLPFALPTAVAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
IALTALYGGKGWVGRWLEAIGIKVAYTQLGIVLALVFVGLPFVVRVVQPVLAESDREVEA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AAATLGASRWQTVWRVVLPGVWPAVLTGFALAFARGVGEYGSVIFIAGNLPNSTEIAPLL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
ITIRLEEFDYAGATAIAAAMLLLSFVILLVVNTLQARLLQYQRRPA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]