Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 84625103

GI number: 84625103

Start: 3903204

End: 3904049

Strand: Reverse

Name: hemK [H]

Synonym: XOO_3446

Alternate gene names: 84625103

Gene position: 3904049-3903204 (Counterclockwise)

Preceding gene: 84625109

Following gene: 84625101

Centisome position: 79.03

GC content: 69.27

Gene sequence:

>846_bases
ATGTCCGACGATCTCGCTGCCATTCCAGCCGATCCGGTGCTGCGGCGCGCCGCCGAACGGATCGAGCGCTGTGATGCCGA
GGCGCTGCTGCTGCATGCGCTGGGGCGTGATCGCGCCTGGTTGTTCATGCACGGGCGCGATGCGGTGCCTCTATCGGTCG
CGCAGGCTTTCGAGGCGTTGGTGCAGCGCCGCGAGGCCGGCGAGCCGGTCGCCTACCTGACCGGCTCGCGCGGATTCTGG
ACGCTGGACCTGGCCGTCTCGCCGGCCACGCTGATCCCGCGCGCTGACACTGAGCTATTGGTCGAGCTGGCGCTGGAACG
CTTGGACACCTCGCCGGGCCGTCGAGCCGCCGATCTGGGCACCGGCAGCGGCGCGATTGCGCTGGCCATTGCCAGCGAAC
GTCCGCAGTCGCAGCTGATCGCCACCGATGCCAGCGCAGCGGCGCTGGGGATGGCGCGTCGCAACGCGGATCGCCACAGC
TTGCCCAATGTCGACTTCCGGCTGGGCAACTGGTTCGCGCCATTGGCGGGGGAGGCCTTCGACTTAATTGCCAGCAACCC
ACCTTATATCGCCGCGCACGACCCGCATCTACAGCAGGGCGATCTGCGCTACGAACCGGCAAGTGCGCTGGCCTCCGGCA
ACGACGGGCTGGACGATATCCGCCTGATCGTCGCCGACGCGCCTGCGCATCTGCTGCCTGGCGGCTGGCTGCTGCTGGAA
CATGGCTGGGACCAGGGGGATGCGGTGCGGGCATTGCTGGCCGCTTCCGGTTTCGACGCGGCGGCGACCTACCAGGACCT
GGAGGCGCGCGACCGGGTCACGCTAGGGCGCAAACCGCGGGCCTGA

Upstream 100 bases:

>100_bases
GGATGGGGCTATCGATTCTTCAGGCGGAATCTAGTGCAACCGTGGCGTACGCCATCATTGATAATGGTCAGCCGATGTTG
CGCATGAGTGAACAGTCCGT

Downstream 100 bases:

>100_bases
GAGCAGCTGACAAAACTGATGCCGGGACTGAACACCTGACCGTTTTAAGTCGGCGAATTCAACTCTGATTCGCAACGCTT
TTGAGGACCTCCTCGGAGAA

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSDDLAAIPADPVLRRAAERIERCDAEALLLHALGRDRAWLFMHGRDAVPLSVAQAFEALVQRREAGEPVAYLTGSRGFW
TLDLAVSPATLIPRADTELLVELALERLDTSPGRRAADLGTGSGAIALAIASERPQSQLIATDASAAALGMARRNADRHS
LPNVDFRLGNWFAPLAGEAFDLIASNPPYIAAHDPHLQQGDLRYEPASALASGNDGLDDIRLIVADAPAHLLPGGWLLLE
HGWDQGDAVRALLAASGFDAAATYQDLEARDRVTLGRKPRA

Sequences:

>Translated_281_residues
MSDDLAAIPADPVLRRAAERIERCDAEALLLHALGRDRAWLFMHGRDAVPLSVAQAFEALVQRREAGEPVAYLTGSRGFW
TLDLAVSPATLIPRADTELLVELALERLDTSPGRRAADLGTGSGAIALAIASERPQSQLIATDASAAALGMARRNADRHS
LPNVDFRLGNWFAPLAGEAFDLIASNPPYIAAHDPHLQQGDLRYEPASALASGNDGLDDIRLIVADAPAHLLPGGWLLLE
HGWDQGDAVRALLAASGFDAAATYQDLEARDRVTLGRKPRA
>Mature_280_residues
SDDLAAIPADPVLRRAAERIERCDAEALLLHALGRDRAWLFMHGRDAVPLSVAQAFEALVQRREAGEPVAYLTGSRGFWT
LDLAVSPATLIPRADTELLVELALERLDTSPGRRAADLGTGSGAIALAIASERPQSQLIATDASAAALGMARRNADRHSL
PNVDFRLGNWFAPLAGEAFDLIASNPPYIAAHDPHLQQGDLRYEPASALASGNDGLDDIRLIVADAPAHLLPGGWLLLEH
GWDQGDAVRALLAASGFDAAATYQDLEARDRVTLGRKPRA

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=237, Percent_Identity=35.4430379746835, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787463, Length=255, Percent_Identity=50.1960784313725, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI87082085, Length=186, Percent_Identity=34.9462365591398, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582226, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30052; Mature: 29921

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDLAAIPADPVLRRAAERIERCDAEALLLHALGRDRAWLFMHGRDAVPLSVAQAFEAL
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
VQRREAGEPVAYLTGSRGFWTLDLAVSPATLIPRADTELLVELALERLDTSPGRRAADLG
HHHHHCCCCEEEEECCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCCEECCCC
TGSGAIALAIASERPQSQLIATDASAAALGMARRNADRHSLPNVDFRLGNWFAPLAGEAF
CCCCEEEEEEECCCCCHHEEECCCHHHHHHHHHCCCCCCCCCCCEEECCCEECCCCCCHH
DLIASNPPYIAAHDPHLQQGDLRYEPASALASGNDGLDDIRLIVADAPAHLLPGGWLLLE
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEE
HGWDQGDAVRALLAASGFDAAATYQDLEARDRVTLGRKPRA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure 
SDDLAAIPADPVLRRAAERIERCDAEALLLHALGRDRAWLFMHGRDAVPLSVAQAFEAL
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
VQRREAGEPVAYLTGSRGFWTLDLAVSPATLIPRADTELLVELALERLDTSPGRRAADLG
HHHHHCCCCEEEEECCCCEEEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCCEECCCC
TGSGAIALAIASERPQSQLIATDASAAALGMARRNADRHSLPNVDFRLGNWFAPLAGEAF
CCCCEEEEEEECCCCCHHEEECCCHHHHHHHHHCCCCCCCCCCCEEECCCEECCCCCCHH
DLIASNPPYIAAHDPHLQQGDLRYEPASALASGNDGLDDIRLIVADAPAHLLPGGWLLLE
HHHCCCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEE
HGWDQGDAVRALLAASGFDAAATYQDLEARDRVTLGRKPRA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]