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The map label for this gene is insH10 [H]

Identifier: 84624597

GI number: 84624597

Start: 3325058

End: 3326041

Strand: Direct

Name: insH10 [H]

Synonym: XOO_2940

Alternate gene names: 84624597

Gene position: 3325058-3326041 (Clockwise)

Preceding gene: 84624595

Following gene: 84624599

Centisome position: 67.31

GC content: 61.08

Gene sequence:

>984_bases
ATGCAACTGACCTTCGGAGACGCGGAGTACAACGGCAAGCGCAAGCGGACGCGGCGTGAGGTGTTTTTGGCCGAGATGGA
TCAGGTCGTGCCATGGAAGGGCCTGCTGGCGCTGATCGAGCCGCACTATCCAAAGTCGGGGCAGCCGGGGCGACAGCCGT
ATCGGCTGGAGACGATGCTGCGCATCCACTTTTTGCAGCAGTGGTATGCGCTGAGCGATCCCTCGGCGGAAGAAGCGCTG
TACGACACGGTGTCGATGCGCCGCTTCGCGAAGATCGGCGGGCTGGACGAGGTGCCGGATGAAACGACGATTCTCCACTT
CCGGCATCTGCTGGAGCGGCATGATCTGGCGCGCAAGCTGTTCAACCGGGTCAACGCGCACCTGTCGCGTAAAGGGCAGA
GTCTGCGGGGCGGGACGATCGTGGATACCACGATCATTGCGGCGCCCAGTTCGACCAAGAACAAGCAGGGCGAGCGCGAT
CCGGACATGCACCAGACCAAGAAGGGGAATCAATATGACTTCGGGATGAAGGCGCACATCGGGGTGGACGATGACTCCGG
GCTGGTGCACCACGTCGAATGCACGGCGGCCAACGTGGCCGATATCACGCAAGCGCACAAGCTGCTGCACGGCAAGGAAG
ACACGGTGTGCGGGGACAGCGGCTACACCGGGCTTGAGAAGCGCGAGGAAATGAAGCGCAAGCGCACGCTGCGCTACCTG
ATCGCCGAGAAACCCTCGAAGCTGAAGCAGATCAAGAACAAACGCGAACTGAAGTTGGCCAAGCGCTGGGAGCACACCAA
GGCCAGCCTGCGGGCGAAGGTGGAACACCCGTTCCGGGTGATCAAGCGTCAGTTTGGTTACGTCAAGGTGCGCTATCGCG
GCCTGGTCAAGAACACCGCGCAGATGCTAACGCTGTTTGCGTTGTCGAATCTGTGGCTGAAGCGCAAAGAGTTAATGCCC
GCTGCGGGGAAGGTGTGCCTGTAA

Upstream 100 bases:

>100_bases
CAGCTTGCTTCTCAGGATCGGTTGTCTCAAGGATTAGGGAAACTCTGAACACCTCCCCCTGATCCTGCGACAATCGCACA
GACGCAACGTGACGACGACG

Downstream 100 bases:

>100_bases
CACAGGAAATACACCGAAAACGCGGCGAAAACGGCAAAAAATCAGGGGTTCGAGCGCCCTCAGCGCCGCAGATGTGGCTT
GCTTCATTTCCCGGCCGCGT

Product: ISXoo6 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFNRVNAHLSRKGQSLRGGTIVDTTIIAAPSSTKNKQGERD
PDMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYL
IAEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRGLVKNTAQMLTLFALSNLWLKRKELMP
AAGKVCL

Sequences:

>Translated_327_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFNRVNAHLSRKGQSLRGGTIVDTTIIAAPSSTKNKQGERD
PDMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYL
IAEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRGLVKNTAQMLTLFALSNLWLKRKELMP
AAGKVCL
>Mature_327_residues
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETMLRIHFLQQWYALSDPSAEEAL
YDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKLFNRVNAHLSRKGQSLRGGTIVDTTIIAAPSSTKNKQGERD
PDMHQTKKGNQYDFGMKAHIGVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYL
IAEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRGLVKNTAQMLTLFALSNLWLKRKELMP
AAGKVCL

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=312, Percent_Identity=56.4102564102564, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788303, Length=312, Percent_Identity=55.7692307692308, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789921, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789612, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789355, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788518, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788342, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1787592, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1786877, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1786764, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1786453, Length=312, Percent_Identity=55.4487179487179, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 37636; Mature: 37636

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETML
CEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RIHFLQQWYALSDPSAEEALYDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FNRVNAHLSRKGQSLRGGTIVDTTIIAAPSSTKNKQGERDPDMHQTKKGNQYDFGMKAHI
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEE
GVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYL
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IAEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRGLVKNTA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHH
QMLTLFALSNLWLKRKELMPAAGKVCL
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQLTFGDAEYNGKRKRTRREVFLAEMDQVVPWKGLLALIEPHYPKSGQPGRQPYRLETML
CEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
RIHFLQQWYALSDPSAEEALYDTVSMRRFAKIGGLDEVPDETTILHFRHLLERHDLARKL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FNRVNAHLSRKGQSLRGGTIVDTTIIAAPSSTKNKQGERDPDMHQTKKGNQYDFGMKAHI
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEE
GVDDDSGLVHHVECTAANVADITQAHKLLHGKEDTVCGDSGYTGLEKREEMKRKRTLRYL
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IAEKPSKLKQIKNKRELKLAKRWEHTKASLRAKVEHPFRVIKRQFGYVKVRYRGLVKNTA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHH
QMLTLFALSNLWLKRKELMPAAGKVCL
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]