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The map label for this gene is dgoD [H]

Identifier: 84624426

GI number: 84624426

Start: 3120959

End: 3122107

Strand: Direct

Name: dgoD [H]

Synonym: XOO_2769

Alternate gene names: 84624426

Gene position: 3120959-3122107 (Clockwise)

Preceding gene: 84624425

Following gene: 84624427

Centisome position: 63.17

GC content: 64.58

Gene sequence:

>1149_bases
ATGAAGATCACCCGCCTCACCACCTACCACGCTGCGCCGCGCTGGTTGTTCCTCAAGGTCGAAACCGACGAGGGCATTAC
CGGTTGGGGAGAGCCGGTTATCGAAGGCCGCGCCCGTTCGGTGGAGGCCGCCGTACACGAACTGGCCGGCTATGTCGTCG
GCAAGGATCCGGCGCGCATCAACGACCTGTGGCAGACCATGTACCGCGCCGGTTTCTACCGTGGCGGCGCCATCCTGATG
AGCGCGATCGCCGGTATCGACCAGGCGTTGTGGGACATCAAGGGCAAGGCGCTGGGCGTGCCGGTATACGAGTTGCTGGG
CGGGCTGGTGCGCGACCGCATGAAGACCTATCGCTGGGTCGGCGGCGACCGCCCGGGCGCGATCATCCAGCAGATCACCG
ACTACCGCGCGCTGGGTTTCGACACCTTCAAGTTCAACGGCACCGAAGAAATGAAGCTGATCGACAGCGCGCGCGCGGTC
GACGCTGCGGTGGTCAAGGTGGCCGAAATCCGCGAAGCCTTCGGCAACACCATCGACTTCGGCATCGACTTCCACGGCCG
CGTCGGCGCGCCGATGGCCAAGGCGTTGCTGCGCGAACTCGAGCCGTTCAAGCCGCTGTTCGTCGAAGAGCCGGTGCTGG
CCGAACAGGCCGAGTACTACCCGCGCCTGGCCGCCAGCACCTCCATTCCGCTGGCCGCCGGCGAGCGCATGTTCTCGCGT
TTCGAATTCAAGAACGTGCTGTGTGCCGGCGGCATCGGCATGGTGCAGCCGGACCTGTCGCATGCCGGCGGCATCACCGA
ATGCGTCAAGATCGCCGCGATCGCCGAAGCCTACGATGTCGGCTTCGCGCCGCACTGCCCGCTCGGCCCGATCGCGCTGG
CTGCATGCCTGCACGTGGACTTCGTCTCGCACAATGCGGTGCTGCAGGAACAGAGCATCGGCATTCATTACAACGAAGGC
GCCGACCTGCTCGATTACGTCATCAACAAAGACGATTTTCACTGCGTGGATGGCAGCATTGCCGCCCTGCCCAAACCCGG
GCTCGGTGTGGAGATCGACGAAGACATGCTCAAGCGCGCAAACGAAAACCCGCCCGACTGGCGCAACCCGGTGTGGCGCC
ACAGCGATGGCAGCATCGCCGAATGGTGA

Upstream 100 bases:

>100_bases
GCGCCGGCATCGGCGGCGAACTGTACAAACCGGCGCAATCGCTCGAAACAACCCAGACGCATGCACGCGCCTTCGTGCAG
GCCTACCAGGAACTGCAAGG

Downstream 100 bases:

>100_bases
GCACGCCCGAACACACCGCCGTGCTGGCGGTCGACAGCCGCTGCACGCACGGCGAAGGCGTGCTGTGGTGCGCACGGCGC
GAGGCGCTGTACTGGGTCGA

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTMYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIREAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAIAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVINKDDFHCVDGSIAALPKPGLGVEIDEDMLKRANENPPDWRNPVWRHSDGSIAEW

Sequences:

>Translated_382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTMYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIREAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAIAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVINKDDFHCVDGSIAALPKPGLGVEIDEDMLKRANENPPDWRNPVWRHSDGSIAEW
>Mature_382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTMYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIREAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAIAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVINKDDFHCVDGSIAALPKPGLGVEIDEDMLKRANENPPDWRNPVWRHSDGSIAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=73.8219895287958, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=29.5399515738499, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI226510960, Length=366, Percent_Identity=27.5956284153005, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 41926; Mature: 41926

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARI
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDLWQTMYRAGFYRGGAILMSAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAVDAAVVKVAEIREAFGNTIDF
CCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAIAEAYDVGFAPHCPLGPIALAACLHVD
HHHHHHHEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FVSHNAVLQEQSIGIHYNEGADLLDYVINKDDFHCVDGSIAALPKPGLGVEIDEDMLKRA
HHHCCHHEECCCCCEEECCCCHHHHHHHCCCCCEEECCCEEECCCCCCCCEECHHHHHHC
NENPPDWRNPVWRHSDGSIAEW
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARI
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDLWQTMYRAGFYRGGAILMSAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAVDAAVVKVAEIREAFGNTIDF
CCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
GIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAIAEAYDVGFAPHCPLGPIALAACLHVD
HHHHHHHEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FVSHNAVLQEQSIGIHYNEGADLLDYVINKDDFHCVDGSIAALPKPGLGVEIDEDMLKRA
HHHCCHHEECCCCCEEECCCCHHHHHHHCCCCCEEECCCEEECCCCCCCCEECHHHHHHC
NENPPDWRNPVWRHSDGSIAEW
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]