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The map label for this gene is insF1 [C]

Identifier: 84624323

GI number: 84624323

Start: 2998260

End: 2999114

Strand: Direct

Name: insF1 [C]

Synonym: XOO_2666

Alternate gene names: 84624323

Gene position: 2998260-2999114 (Clockwise)

Preceding gene: 84624322

Following gene: 84624327

Centisome position: 60.69

GC content: 62.57

Gene sequence:

>855_bases
ATGCAAGCCCACCGCGAAGAATTCAGGGTGTGCGCGATGTGCCGGGTGTTGCGGGTGAACCGGGCTGGATACGACGCGTG
GCTAAAGTCGCCCGACAGTGAGCGCGCCAAGGAAGACGAACGCCTGCTGGGGCTGATCAAGCACCACTGGCTGGCCAGCG
GCAGTGTGTATGGGCATCGCAAGATCGCCAAGGATCTGCGCGATCTGGGTGAGCGTTGCAGTCGCCATCGGGTGCATCGG
CTGATGCGCGCCGATGGGCTGCGTGCCCAGGTGGGCTATGGTCGCAAACCGCGCTTCCATGGCGGAATGCCGTGCAAGGC
GGCAGCCAACCTGCTGGATCGGCAGTTCGAGGTGACCGAGCCGGATACGGCCTGGGCGAGCGACTTCACCTTTATCCGTA
CGCATGAAGGCTGGATGTACCTGGCTGTGGTGATCGATCTGTTTTCCAGGCAGGTCGTCGGCTGGGCGATGCGCGATCGA
GCCGATACCGAGTTGGCCGTGCAGGCCTTGCTGTCGGCGGTGTGGCGGCGCAAGCCCAGCGCCGGTTGCCTGGTTCACTC
GGACCAAGGGTCGGTCTACACCAGCGATGACTGGCGCAGTTTCCTGGTGTCCCACGGTTTGGTGTGCAGCATGAGTCGAC
GTGGCAACTGCCATGACAATGCCCCGGTGGAGAGCTTCTTCGGTCTGCTCAAACGCGAGCGGATCAGGCGGCGGATCTAC
CCCACCAAGGACGCCGCACGCGCCGAGGTGTTCGACGACATCGAAATGTTCTACAACCCACAACGCCGTCATGGTTCAAC
AGGCAACCTATCGCCTGTAGAGTTTCAACGGCGCTACGCGCAACGAGGGTCTTGA

Upstream 100 bases:

>100_bases
GGCGGCTGAAGACCGAGTTGCGCCGGGTGACGGAGGAGCGCGACATCCTAAAAAAGGCCGTCGCGTACTTTGCCAAGGGG
TAAAGGCAAAGTACGTCCTC

Downstream 100 bases:

>100_bases
GTGTCTACGGAACCCTGGGCGTATCAAACGCTGGCGCCGCGAATAAAACCAACACCGCCCAAAGAAAGCAATCGGCGCAA
TGACGCCGGCAACGTATGCC

Product: ISXoo9 transposase orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQAHREEFRVCAMCRVLRVNRAGYDAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHR
LMRADGLRAQVGYGRKPRFHGGMPCKAAANLLDRQFEVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDR
ADTELAVQALLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLVSHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIY
PTKDAARAEVFDDIEMFYNPQRRHGSTGNLSPVEFQRRYAQRGS

Sequences:

>Translated_284_residues
MQAHREEFRVCAMCRVLRVNRAGYDAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHR
LMRADGLRAQVGYGRKPRFHGGMPCKAAANLLDRQFEVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDR
ADTELAVQALLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLVSHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIY
PTKDAARAEVFDDIEMFYNPQRRHGSTGNLSPVEFQRRYAQRGS
>Mature_284_residues
MQAHREEFRVCAMCRVLRVNRAGYDAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHR
LMRADGLRAQVGYGRKPRFHGGMPCKAAANLLDRQFEVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDR
ADTELAVQALLSAVWRRKPSAGCLVHSDQGSVYTSDDWRSFLVSHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIY
PTKDAARAEVFDDIEMFYNPQRRHGSTGNLSPVEFQRRYAQRGS

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=240, Percent_Identity=33.75, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1788405, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787263, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786753, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786570, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786490, Length=280, Percent_Identity=39.2857142857143, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789981, Length=219, Percent_Identity=32.4200913242009, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32780; Mature: 32780

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAHREEFRVCAMCRVLRVNRAGYDAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHR
CCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KIAKDLRDLGERCSRHRVHRLMRADGLRAQVGYGRKPRFHGGMPCKAAANLLDRQFEVTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELAVQALLSAVWRRKPS
CCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
AGCLVHSDQGSVYTSDDWRSFLVSHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIY
CCEEEECCCCCEEECHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PTKDAARAEVFDDIEMFYNPQRRHGSTGNLSPVEFQRRYAQRGS
CCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQAHREEFRVCAMCRVLRVNRAGYDAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHR
CCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
KIAKDLRDLGERCSRHRVHRLMRADGLRAQVGYGRKPRFHGGMPCKAAANLLDRQFEVTE
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
PDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELAVQALLSAVWRRKPS
CCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
AGCLVHSDQGSVYTSDDWRSFLVSHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIY
CCEEEECCCCCEEECHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PTKDAARAEVFDDIEMFYNPQRRHGSTGNLSPVEFQRRYAQRGS
CCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]