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The map label for this gene is puuA [H]

Identifier: 84624282

GI number: 84624282

Start: 2928267

End: 2929646

Strand: Direct

Name: puuA [H]

Synonym: XOO_2625

Alternate gene names: 84624282

Gene position: 2928267-2929646 (Clockwise)

Preceding gene: 84624279

Following gene: 84624283

Centisome position: 59.27

GC content: 66.38

Gene sequence:

>1380_bases
ATGGCTACCGAGAACCCCATCGCGTCCCTGCCCGCTGCCGATGCCGACACCTTGGTGCGCATCGCCGGTTGCGAAGCAGT
CGATCTGTTATTGCCCGACACCAACGGCGTGCTGCGCGGCAAGCGCGTGACCGCCGAGGCGCTGAGCAAGGTCTATCGCG
ATGGTGTGTGCCTGCCGATGTCGCTGATCGCCACCGACATCACCGGCAACACCGTGGAAGAAACCGGGCTTGGCTACGCC
ATCGGCGATGCCGACCGCATCTGCCGGCCGATCGAAGGCTCGCTACGCCCGGTGCCGTGGGCGCCACGGCCGATGGCGCA
GTTGCTGCTGGCGATGCATACCCCCGAAGGCGGGTTGTTCGACGTGGCGCCACGTGCGGTGTTGCAGCGCGTGCTGCAAG
GATTTGGCGCGCTCGGCCTGAACCCGGTGATCGCCGTAGAACTGGAGTTCTACCTGTTCGATGCGGTGGCCGATGCGCAG
GGGCGCCCGCAGACGCCGCGCGACCCGCTCACCGGCGAACGCAACGACAGCACCCAGGTGTATTCGCTGCAGGATCTGGA
CGACCAGCGCGGCTTCACCGATGCGGTCACAGCGGCTTGCCGGCAGCAAGGCATTCCGGCCGACACTGCCGTTGCCGAAT
ACGCGCCCGGCCAGTTCGAAATCAATCTGCAACATCGCGCCGATGCGGTAGCCGCCTGCGATGAGGCGATCCTGCTCAAG
CGCACCATCAAGGCGATTGCCCAGCAGCAGGGCAAGCTTGCCAGCTTCATGGCCAAGCCGTTCCCCGGCCAGGCCGGCAG
CGGGCTGCATCTGCACGTAAGCCTGCGCGATGGCACCGGGCACAACGTGCTGGCCAGCGCTGCCGATGCGCCTGCAGACC
CGCTGCGCCACGCCATCGCCGGGCTGCAGCGGCATGCCGATGCCGCGCTCCTGATGTTCGCCCCGCATGCCAACAGCTAT
CGCCGCTTCGTCAGCAATGCCTTCGTGCCGCTGGATGCGAGCTGGGGTTTCAACAACCGCACTGTGGCGCTGCGCATCCC
ACATAGCGATGCACACAACACCCGTATCGAGCACCGCATTGCCGGCGCCGATGCCAACCCATATCTGGCGGCTGCAGCGG
TGCTGGCCGCGATGCTGGACGGCCTGCGCAGGCCTGCAGAACCCACTGCGCCCATCAGCGGCAATGCGTATGCGCAGTCG
GGGTTCTCCCCGCGCACCTGGCAAGGCGCGGTGGATGCGTTCCTGAGCAGCGACTTCATCGGCACGCAGTTCGGCACCCA
GTTCCAGCATGTGTTCGGCCAGCAAAAACAGCGCGAGTTGCTCGACTACCAGGCACTGGTGCCCGACCTGGATTACGCGC
GGTACCTGCGCACCGTGTGA

Upstream 100 bases:

>100_bases
CTTACCGCGCCATCGCCGGCATTGCCAAGTGCGCCGTTGCGCTTGCGATTGCTCACGTGCGCTTTGCTACGCTGCGCGAC
GACTTCGCAGATGTTGCCAC

Downstream 100 bases:

>100_bases
TGACTCGCCCGCACGCGCCCCAGCCGCGCAGCGATCCACCGGACAGCGACTACCCGGACAGCTTGTACGCGCGCAGCACG
CCGGCCCTGCCCCGGCAGCC

Product: glutamine synthase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MATENPIASLPAADADTLVRIAGCEAVDLLLPDTNGVLRGKRVTAEALSKVYRDGVCLPMSLIATDITGNTVEETGLGYA
IGDADRICRPIEGSLRPVPWAPRPMAQLLLAMHTPEGGLFDVAPRAVLQRVLQGFGALGLNPVIAVELEFYLFDAVADAQ
GRPQTPRDPLTGERNDSTQVYSLQDLDDQRGFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEAILLK
RTIKAIAQQQGKLASFMAKPFPGQAGSGLHLHVSLRDGTGHNVLASAADAPADPLRHAIAGLQRHADAALLMFAPHANSY
RRFVSNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLAAAAVLAAMLDGLRRPAEPTAPISGNAYAQS
GFSPRTWQGAVDAFLSSDFIGTQFGTQFQHVFGQQKQRELLDYQALVPDLDYARYLRTV

Sequences:

>Translated_459_residues
MATENPIASLPAADADTLVRIAGCEAVDLLLPDTNGVLRGKRVTAEALSKVYRDGVCLPMSLIATDITGNTVEETGLGYA
IGDADRICRPIEGSLRPVPWAPRPMAQLLLAMHTPEGGLFDVAPRAVLQRVLQGFGALGLNPVIAVELEFYLFDAVADAQ
GRPQTPRDPLTGERNDSTQVYSLQDLDDQRGFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEAILLK
RTIKAIAQQQGKLASFMAKPFPGQAGSGLHLHVSLRDGTGHNVLASAADAPADPLRHAIAGLQRHADAALLMFAPHANSY
RRFVSNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLAAAAVLAAMLDGLRRPAEPTAPISGNAYAQS
GFSPRTWQGAVDAFLSSDFIGTQFGTQFQHVFGQQKQRELLDYQALVPDLDYARYLRTV
>Mature_458_residues
ATENPIASLPAADADTLVRIAGCEAVDLLLPDTNGVLRGKRVTAEALSKVYRDGVCLPMSLIATDITGNTVEETGLGYAI
GDADRICRPIEGSLRPVPWAPRPMAQLLLAMHTPEGGLFDVAPRAVLQRVLQGFGALGLNPVIAVELEFYLFDAVADAQG
RPQTPRDPLTGERNDSTQVYSLQDLDDQRGFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEAILLKR
TIKAIAQQQGKLASFMAKPFPGQAGSGLHLHVSLRDGTGHNVLASAADAPADPLRHAIAGLQRHADAALLMFAPHANSYR
RFVSNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRIAGADANPYLAAAAVLAAMLDGLRRPAEPTAPISGNAYAQSG
FSPRTWQGAVDAFLSSDFIGTQFGTQFQHVFGQQKQRELLDYQALVPDLDYARYLRTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=418, Percent_Identity=24.4019138755981, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081870, Length=442, Percent_Identity=42.9864253393665, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1790301, Length=300, Percent_Identity=28.3333333333333, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49255; Mature: 49124

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATENPIASLPAADADTLVRIAGCEAVDLLLPDTNGVLRGKRVTAEALSKVYRDGVCLPM
CCCCCCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCH
SLIATDITGNTVEETGLGYAIGDADRICRPIEGSLRPVPWAPRPMAQLLLAMHTPEGGLF
HHHHCCCCCCCHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
DVAPRAVLQRVLQGFGALGLNPVIAVELEFYLFDAVADAQGRPQTPRDPLTGERNDSTQV
CCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCEE
YSLQDLDDQRGFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEAILLK
EEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
RTIKAIAQQQGKLASFMAKPFPGQAGSGLHLHVSLRDGTGHNVLASAADAPADPLRHAIA
HHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHH
GLQRHADAALLMFAPHANSYRRFVSNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRI
HHHHCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHEE
AGADANPYLAAAAVLAAMLDGLRRPAEPTAPISGNAYAQSGFSPRTWQGAVDAFLSSDFI
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCC
GTQFGTQFQHVFGQQKQRELLDYQALVPDLDYARYLRTV
CCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
ATENPIASLPAADADTLVRIAGCEAVDLLLPDTNGVLRGKRVTAEALSKVYRDGVCLPM
CCCCCCCCCCCCCHHHEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCH
SLIATDITGNTVEETGLGYAIGDADRICRPIEGSLRPVPWAPRPMAQLLLAMHTPEGGLF
HHHHCCCCCCCHHHCCCCEEECCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
DVAPRAVLQRVLQGFGALGLNPVIAVELEFYLFDAVADAQGRPQTPRDPLTGERNDSTQV
CCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCEE
YSLQDLDDQRGFTDAVTAACRQQGIPADTAVAEYAPGQFEINLQHRADAVAACDEAILLK
EEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHH
RTIKAIAQQQGKLASFMAKPFPGQAGSGLHLHVSLRDGTGHNVLASAADAPADPLRHAIA
HHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHH
GLQRHADAALLMFAPHANSYRRFVSNAFVPLDASWGFNNRTVALRIPHSDAHNTRIEHRI
HHHHCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHEE
AGADANPYLAAAAVLAAMLDGLRRPAEPTAPISGNAYAQSGFSPRTWQGAVDAFLSSDFI
ECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCC
GTQFGTQFQHVFGQQKQRELLDYQALVPDLDYARYLRTV
CCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]