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The map label for this gene is puuB [H]

Identifier: 84624283

GI number: 84624283

Start: 2929646

End: 2930962

Strand: Direct

Name: puuB [H]

Synonym: XOO_2626

Alternate gene names: 84624283

Gene position: 2929646-2930962 (Clockwise)

Preceding gene: 84624282

Following gene: 84624284

Centisome position: 59.3

GC content: 70.46

Gene sequence:

>1317_bases
ATGACTCGCCCGCACGCGCCCCAGCCGCGCAGCGATCCACCGGACAGCGACTACCCGGACAGCTTGTACGCGCGCAGCAC
GCCGGCCCTGCCCCGGCAGCCGCGGCTGAAAGGCGCCGAACATGCCGATGTCTGCATCCTCGGCGGTGGCTATACCGGCC
TGACCGCCGCGCTGGCGCTGGCCGAGGCCGGTTACTCCGTCATCGTGCTGGAAGCCCGGCGCGTAGGCTGGGGTGCGTCC
GGGCGCAACGGTGGCCAGGCCATCGTCGGCTACGGCTGCGAGCAGGAGACGCTGGAGGCGCTGGTCGGCAACGACGCTGC
ACGCCTGCTGTTCGATTTCTCGCGCGATGGCATGCGCCTGCTGCGCGCGCGCATCGCCCGCCATGCCATCGCCTGCGACT
GGCGCGATGGGCACGCGCATGTTCCGCTAAAGCCGCGCCAGGTACGGGCGCTGCAGCACGGCATCGTGCGCATGGCCGAA
CGCTACGACTACCCGCTGCAATGGTGGGACCGTGCGCGCACCCGCCAGGTGCTGGACAGCCCGCTGTATCTGGGCGCCAT
GTTCGACCCTGCCAGTGGCCATCTGCATCCGCTGGCCTATGCACTGGGCCTGGCCCGTGCCGCGCTGGCGGCCGGGGTGC
GCATCTACGAAGACTCGGCCGTGACGCAGCAGCAGGCCGGCACGCGCGTGGTCATGCACACCGCACACGGCAGCGTCACC
GCCAGCTTCGGGGTGATCGCCGGCAATGCCCTGCTGCACGGCATCGCCCCGACGCTGGAGCAGCGCATCATGCCGGTGGG
CACCTATGTCGGCGCCACCCCGCCCCTGGGCGAAGCGCGCGCACGCGCCCTGATTGCCAACGACATGGCGGTGGCCGACA
CCAACTGGGCACTGGATTACTATCGGCTCAGCGCCGACCACCGCCTGCTGTTCGGTGGGCGTGCCAGTTACTCTTCGCGT
CCGCCCGCGGGCCTGCAGCACCTGATGACCCAGCGCATGCACACGGTGTTTCCGCAGCTGCACGATGTGTCGCTGGAAAT
CCTGTGGGGTGGCTACGTGGACATCTCGCGCAACCGTGCGCCGCACTGGGGCCGGCTGGCGCCCAACCTGTACTTCGCGC
AGGGCTTCTCCGGGCACGGCGTCGCTGCCACGGGCCTGGCTGGCCAGGTCATCGCCGAGGCCATCGCCGGCCAGAGCCGG
CGCCTGGACGTGTTCGAACGCATCGGGCATCGCCCGTTTCCCGGCGGGCAGCGACTGCGCACCCCACTGCTGGTGGCGGC
GATGAGCTGGCATCGGCTGCGCGATGCACTGTGGTGA

Upstream 100 bases:

>100_bases
AGTTCCAGCATGTGTTCGGCCAGCAAAAACAGCGCGAGTTGCTCGACTACCAGGCACTGGTGCCCGACCTGGATTACGCG
CGGTACCTGCGCACCGTGTG

Downstream 100 bases:

>100_bases
CCGGCGCGTTGCAGCCACGTGCTCAAGTTTTTGCCAGCAGGGCCGTTAGCTGACCTGACGGTTCGATCCAGACGGCCAAC
TGATGTCCCCAAGTAAAGTC

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTRPHAPQPRSDPPDSDYPDSLYARSTPALPRQPRLKGAEHADVCILGGGYTGLTAALALAEAGYSVIVLEARRVGWGAS
GRNGGQAIVGYGCEQETLEALVGNDAARLLFDFSRDGMRLLRARIARHAIACDWRDGHAHVPLKPRQVRALQHGIVRMAE
RYDYPLQWWDRARTRQVLDSPLYLGAMFDPASGHLHPLAYALGLARAALAAGVRIYEDSAVTQQQAGTRVVMHTAHGSVT
ASFGVIAGNALLHGIAPTLEQRIMPVGTYVGATPPLGEARARALIANDMAVADTNWALDYYRLSADHRLLFGGRASYSSR
PPAGLQHLMTQRMHTVFPQLHDVSLEILWGGYVDISRNRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAIAGQSR
RLDVFERIGHRPFPGGQRLRTPLLVAAMSWHRLRDALW

Sequences:

>Translated_438_residues
MTRPHAPQPRSDPPDSDYPDSLYARSTPALPRQPRLKGAEHADVCILGGGYTGLTAALALAEAGYSVIVLEARRVGWGAS
GRNGGQAIVGYGCEQETLEALVGNDAARLLFDFSRDGMRLLRARIARHAIACDWRDGHAHVPLKPRQVRALQHGIVRMAE
RYDYPLQWWDRARTRQVLDSPLYLGAMFDPASGHLHPLAYALGLARAALAAGVRIYEDSAVTQQQAGTRVVMHTAHGSVT
ASFGVIAGNALLHGIAPTLEQRIMPVGTYVGATPPLGEARARALIANDMAVADTNWALDYYRLSADHRLLFGGRASYSSR
PPAGLQHLMTQRMHTVFPQLHDVSLEILWGGYVDISRNRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAIAGQSR
RLDVFERIGHRPFPGGQRLRTPLLVAAMSWHRLRDALW
>Mature_437_residues
TRPHAPQPRSDPPDSDYPDSLYARSTPALPRQPRLKGAEHADVCILGGGYTGLTAALALAEAGYSVIVLEARRVGWGASG
RNGGQAIVGYGCEQETLEALVGNDAARLLFDFSRDGMRLLRARIARHAIACDWRDGHAHVPLKPRQVRALQHGIVRMAER
YDYPLQWWDRARTRQVLDSPLYLGAMFDPASGHLHPLAYALGLARAALAAGVRIYEDSAVTQQQAGTRVVMHTAHGSVTA
SFGVIAGNALLHGIAPTLEQRIMPVGTYVGATPPLGEARARALIANDMAVADTNWALDYYRLSADHRLLFGGRASYSSRP
PAGLQHLMTQRMHTVFPQLHDVSLEILWGGYVDISRNRAPHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAIAGQSRR
LDVFERIGHRPFPGGQRLRTPLLVAAMSWHRLRDALW

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=42.688679245283, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47770; Mature: 47639

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPHAPQPRSDPPDSDYPDSLYARSTPALPRQPRLKGAEHADVCILGGGYTGLTAALAL
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHH
AEAGYSVIVLEARRVGWGASGRNGGQAIVGYGCEQETLEALVGNDAARLLFDFSRDGMRL
HHCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHCCHHHHH
LRARIARHAIACDWRDGHAHVPLKPRQVRALQHGIVRMAERYDYPLQWWDRARTRQVLDS
HHHHHHHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
PLYLGAMFDPASGHLHPLAYALGLARAALAAGVRIYEDSAVTQQQAGTRVVMHTAHGSVT
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCEEEEEECCCCEE
ASFGVIAGNALLHGIAPTLEQRIMPVGTYVGATPPLGEARARALIANDMAVADTNWALDY
EEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCEEEE
YRLSADHRLLFGGRASYSSRPPAGLQHLMTQRMHTVFPQLHDVSLEILWGGYVDISRNRA
EEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCEEEEEECCEEEECCCCC
PHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAIAGQSRRLDVFERIGHRPFPGGQRLR
CCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHH
TPLLVAAMSWHRLRDALW
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRPHAPQPRSDPPDSDYPDSLYARSTPALPRQPRLKGAEHADVCILGGGYTGLTAALAL
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHH
AEAGYSVIVLEARRVGWGASGRNGGQAIVGYGCEQETLEALVGNDAARLLFDFSRDGMRL
HHCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHCCHHHHH
LRARIARHAIACDWRDGHAHVPLKPRQVRALQHGIVRMAERYDYPLQWWDRARTRQVLDS
HHHHHHHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
PLYLGAMFDPASGHLHPLAYALGLARAALAAGVRIYEDSAVTQQQAGTRVVMHTAHGSVT
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCEEEEEECCCCEE
ASFGVIAGNALLHGIAPTLEQRIMPVGTYVGATPPLGEARARALIANDMAVADTNWALDY
EEHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCEEEE
YRLSADHRLLFGGRASYSSRPPAGLQHLMTQRMHTVFPQLHDVSLEILWGGYVDISRNRA
EEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCEEEEEECCEEEECCCCC
PHWGRLAPNLYFAQGFSGHGVAATGLAGQVIAEAIAGQSRRLDVFERIGHRPFPGGQRLR
CCCCCCCCCEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHH
TPLLVAAMSWHRLRDALW
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]