Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 84624263

GI number: 84624263

Start: 2906425

End: 2907879

Strand: Direct

Name: miaB [H]

Synonym: XOO_2606

Alternate gene names: 84624263

Gene position: 2906425-2907879 (Clockwise)

Preceding gene: 84624262

Following gene: 84624264

Centisome position: 58.83

GC content: 63.71

Gene sequence:

>1455_bases
ATGCCCGGGACATCCGTTTCCGATCTGTCCACGGCCACCGCCGTGGATGCACCCGCCCTGCTGCCCTTGCCCGTGGCGCG
CCCTTCCGCGCCTGCCGTGGTGCGCGGCAAGCTCTATATCAAGACCCACGGGTGCCAGATGAACGAGTATGACTCGGCAA
AGATGGCCGACGTGCTCGCCGCCTCCGAAGGCCTGGAGCTGACCGATAACCCGGAAGAAGCGGATGTGGTGCTGGTCAAC
ACCTGCTCCATTCGCGAAAAGGCGCAGGAAAAGGTCTTCAGTCAGTTGGGGCGCTGGAAGGCGCTGAAGGCCGGCGGCAA
GCCGGTGATCATCGGCGTCGGCGGCTGTGTGGCGTCGCAGGAAGGCGAAGCCATCGTCAAACGTGCGCCCTATGTAGACC
TGGTGTTCGGCCCGCAGACGCTGCACCGCTTGCCGGAATTGATCCGCGCACGGCGCGAATCGGGCAAGTCGCAGGTGGAC
ATCAGCTTCCCGGAGATCGAGAAGTTCGACCGCCTGCCCGAGCCGCGTGCCGAAGGCCCGTCGGCGTTCGTCTCGATCAT
GGAAGGCTGCTCCAAATACTGCTCGTTCTGCGTGGTGCCTTACACCCGTGGCGAAGAAGTCAGCCGGCCGTTCGAAGACG
TGCTGGTGGAAGTGGCGCAATTGGCGGCGCAAGGCGTGCGCGAGATCAACCTGCTCGGCCAGAACGTCAACGCGTACCGC
GGTGCATATGGCGCCGATGCCGGCGACGCCGCGCAGTACGCCGATCTGGGCCTGCTGATCCGCACCATCGCGCAGATCGA
GGGCATCGGCCGCATCCGCTTCACCACCTCGCATCCGCTGGAGTTTTCCGATTCGCTGGTGGATGCCTACCGCGATGTGC
CGCAGCTGGCCAACTACCTGCACCTGCCGGTACAGGCGGGCAGCGACCGCATTCTGTCGGCGATGAAGCGCGGCTACACC
GCGCTGGAATTCAAATCCAGGATCCGCAAGCTGCGTGCGGTGCGCCCGGATATTTCGATCAGTTCGGATTTCATCGTCGG
CTTCCCCGGCGAAACCGAAGCGGACTTCGAAAAGACCATGAAGCTGATCGAGGACGTGGGCTTCGACCAGAGCTTTTCGT
TCGTGTATTCGCGTCGTCCCGGCACCCCGGCATCGGACCTGCAAGACGACACGCCGGAGACGGTGAAGCAGGCGCGTCTT
GCACGTCTGCAGGCGCACATCAGCGCGCACGCGGCGAGCATTTCGCAATCGATGGTGGGCAGCGTGCAGCGCGTGCTGGT
GGAAGGCCCCTCACGGCGCGACCCGAACGAATTGACCGGCAAGAGCGAAAACATGCGCCCGGTGAATTTCCCGGGCAACC
CGCGTTTGATTGGGCAGTTCGTGGACGTGTTGATCACCGAGGCGATGAGCAATTCGCTGCGTGGTCGCATTCAACTGGAC
GACAGCGCGCACTGA

Upstream 100 bases:

>100_bases
TGGCGCGGGCCCAGGCGGTCGACACCTCCGCTGGGGCGGACGCTGGTGGCGCAAGCGGGTAAAATGCGCGGCTCTCTTCT
ACCCGCCTGGAATAAGCGCC

Downstream 100 bases:

>100_bases
AGACGGGGAGGCACCGCGCCATGCGGCGCTTCTTCCAGCAGCGCAACGACGCGCCGAAGCGTGTCGCGCGCAATGGTCAA
AATTTGACCACACAGCTGTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEEADVVLVN
TCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVD
ISFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYT
ALEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARL
ARLQAHISAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
DSAH

Sequences:

>Translated_484_residues
MPGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEEADVVLVN
TCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVD
ISFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYT
ALEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARL
ARLQAHISAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
DSAH
>Mature_483_residues
PGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEEADVVLVNT
CSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDI
SFPEIEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYRG
AYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYTA
LEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTMKLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARLA
RLQAHISAHAASISQSMVGSVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLDD
SAH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=38.2352941176471, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=38.2352941176471, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=29.2035398230088, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=66.8934240362812, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787057, Length=374, Percent_Identity=30.4812834224599, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=35.1409978308026, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=308, Percent_Identity=30.1948051948052, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=33.7552742616034, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=30.718954248366, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52717; Mature: 52586

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLA
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
ASEGLELTDNPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ
HCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECCC
EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCH
SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
HHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
HLPVQAGSDRILSAMKRGYTALEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTM
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARLARLQAHISAHAASISQSMVG
HHHHHCCCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
DSAH
CCCC
>Mature Secondary Structure 
PGTSVSDLSTATAVDAPALLPLPVARPSAPAVVRGKLYIKTHGCQMNEYDSAKMADVLA
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHH
ASEGLELTDNPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ
HCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEECCC
EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP
CCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCH
SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR
HHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHE
HLPVQAGSDRILSAMKRGYTALEFKSRIRKLRAVRPDISISSDFIVGFPGETEADFEKTM
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPETVKQARLARLQAHISAHAASISQSMVG
HHHHHCCCCCCCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
DSAH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA