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The map label for this gene is eda [H]

Identifier: 84623852

GI number: 84623852

Start: 2434926

End: 2435585

Strand: Direct

Name: eda [H]

Synonym: XOO_2195

Alternate gene names: 84623852

Gene position: 2434926-2435585 (Clockwise)

Preceding gene: 84623851

Following gene: 84623853

Centisome position: 49.29

GC content: 68.03

Gene sequence:

>660_bases
ATGACGATTGCCCAGACCCAGAACACCGCCGAACAGTTGCTGCGCGACGCCGGCATCCTGCCGGTGGTCACCGTGGACAC
GCTGGACCAGGCACGCCGCGTCGCCGACGCGCTGCTCGAAGGCGGCCTGCCCGCGATAGAGCTGACCCTGCGCACGCCGG
TCGCGATCGATGCACTGGCGATGCTCAAGCGCGAGCTGCCCAATATCGTGATCGGTGCCGGCACCGTGCTGAGCGAATTG
CAGCTGCGTCAGTCGGTGGATGCCGGTGCAGACTTTTTGGTCACCCCAGGCACGCCGGCGCCGCTGGCGCGCCTACTGGC
AGATGCGCCGATTCCCGCAGTTCCCGGTGCGGCCACCCCAACCGAGCTGCTGACCCTGATGAGCCTCGGGTTCCGCGTGT
GCAAGCTGTTCCCAGCCACCGCCGTGGGCGGTCTGCAGATGCTCAAGGGCCTGGCCGGCCCGTTGCCTGAGCTCAAGCTG
TGCCCCACCGGCGGCATCAGCGAGACCAACGCGGCCGAGTTCCTGTCGCAGCCGAACGTGCTGTGCATCGGCGGCTCGTG
GATGGTGCCCAAGGACTGGCTTGCGCAGGGCCACTGGGACAAGGTCAAGGAAAGTTCGGCCAAGGCCGCAGCGATCGTGC
GGCAGGTGCGTGCGGGCTGA

Upstream 100 bases:

>100_bases
GACGCAGCCGCTGCAGCCGCACCGCACGAGTCCAAGGACGCCTGATCCTTCTTCCCTCTCTCTGCGGCCGCTGCGGCGGT
CGCCGTCTAACGGAGTTGCA

Downstream 100 bases:

>100_bases
GTTGCTTCTGCAACAGCATCCACCACTGGAAGCCGGCGTTGCAGGGCTTCCAGTTGATGTTCGGCAGAAAGGGTGCCGCT
GGCGGCGATGTGGTGAAAAT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMSLGFRVCKLFPATAVGGLQMLKGLAGPLPELKL
CPTGGISETNAAEFLSQPNVLCIGGSWMVPKDWLAQGHWDKVKESSAKAAAIVRQVRAG

Sequences:

>Translated_219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMSLGFRVCKLFPATAVGGLQMLKGLAGPLPELKL
CPTGGISETNAAEFLSQPNVLCIGGSWMVPKDWLAQGHWDKVKESSAKAAAIVRQVRAG
>Mature_218_residues
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSELQ
LRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMSLGFRVCKLFPATAVGGLQMLKGLAGPLPELKLC
PTGGISETNAAEFLSQPNVLCIGGSWMVPKDWLAQGHWDKVKESSAKAAAIVRQVRAG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=40.8866995073892, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI48994954, Length=200, Percent_Identity=28.5, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 22839; Mature: 22708

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMSLGFRVCKLFPATAVGGLQMLKGLAGPLPELKLCPTGGISETNAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGHWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMSLGFRVCKLFPATAVGGLQMLKGLAGPLPELKLCPTGGISETNAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGHWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]