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The map label for this gene is folD [H]

Identifier: 84623718

GI number: 84623718

Start: 2280085

End: 2280996

Strand: Direct

Name: folD [H]

Synonym: XOO_2061

Alternate gene names: 84623718

Gene position: 2280085-2280996 (Clockwise)

Preceding gene: 84623715

Following gene: 84623719

Centisome position: 46.15

GC content: 67.32

Gene sequence:

>912_bases
ATGACCGCTTCCGCGCCAGCTGCCTCCACTTCGGCCCGCCTTCTCGATGGTCGTCGCATTGCCGAGGAATTGCTCGACGG
ATTGAAGTCGCGGGTGGATGCGCGGCTCGCAGCCGGCAAGGCACGCCCCGGGTTGGCGGTGGTGCTGGTCGGTGGCGACC
CGGCATCGTCGGTGTATGTGCGCAACAAGCGGCGCGCGGCGGAGAAGGTCGGCATCGAAGCCTTCGATTACGACCTGCCC
CAGTGCACCAGCGAAGCCGAGCTGGCGGCGCTGATCGATGAGCTCAATGCCGACCCCAAGATCCACGGCATTTTGATCCA
GCTGCCGTTGCCGGGCATTCCCGATGCCAATCGGCTGATCCAGCGCATCGATCCGCGCAAGGACGTCGATGGGTTCCATC
CGCAGAATGTCGGGCACCTGGCCTTGCGCGAATTCGGGCTGCGCCCGTGCACGCCGCGCGGCATCGTGACCTTGCTCAGC
CACACCGACCAGCCAGTGCGCGGGCGCAATGCCACCATCGTGGGGGTGAGCAACCACGTCGGCCGGCCGATGGGACTGGA
ACTGCTGATTGCCGGTTGCACGGTCACCAGCTGCCACAAATTCACTCCGCCCGATGTGCTGGAAGCCAGCGTGCGCAATG
CCGACATCCTGGTGGTGGCGGTGGGCCGCCCGGGGCTGATTCCGGGCGAATGGGTCAAGCCGGGCGCGGTGGTGATCGAT
GTCGGCATCAACCGGCTCGACGACGGCCGGCTGGTAGGCGACGTCGGCTTCGACGCAGCCGCGCAGCGCGCGGCCTGGAT
CACCCCGGTGCCGGGCGGGGTAGGGCCGATGACGGTCGCCACGCTGATGCAGAACACCATCGAGGCGGCCGATGCGGCCG
GGATTGAGGGTGCGGGAGTCGGGATTCGTTAA

Upstream 100 bases:

>100_bases
CGCTTCGATCAAAGTGGTGCTTGAATCAGAACGTGAGGTGGTGTCGGTCATGACGGAATCGGGGACAATAGACGGCCTTC
GACTCTACACCTGACGCCTC

Downstream 100 bases:

>100_bases
AGGCGACGGCCAAGCGCATGCATCGGCTGATCCGGATTGCATCCAAGATCGGCCGGCCGTGGGCATCCTGACAAACAGGC
GAATGGCGGACGGTGCAAGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTASAPAASTSARLLDGRRIAEELLDGLKSRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QCTSEAELAALIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLS
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIEGAGVGIR

Sequences:

>Translated_303_residues
MTASAPAASTSARLLDGRRIAEELLDGLKSRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLP
QCTSEAELAALIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLS
HTDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIEGAGVGIR
>Mature_302_residues
TASAPAASTSARLLDGRRIAEELLDGLKSRVDARLAAGKARPGLAVVLVGGDPASSVYVRNKRRAAEKVGIEAFDYDLPQ
CTSEAELAALIDELNADPKIHGILIQLPLPGIPDANRLIQRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLSH
TDQPVRGRNATIVGVSNHVGRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVIDV
GINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIEGAGVGIR

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=44.1379310344828, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=43.7710437710438, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.320819112628, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI36796743, Length=208, Percent_Identity=25.9615384615385, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=59.2857142857143, Blast_Score=334, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17568735, Length=256, Percent_Identity=42.578125, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=306, Percent_Identity=39.2156862745098, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=41.1371237458194, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=25.6944444444444, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=47.4576271186441, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=47.6027397260274, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI62472483, Length=299, Percent_Identity=42.1404682274247, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45551871, Length=299, Percent_Identity=42.1404682274247, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=42.6116838487972, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=42.6116838487972, Blast_Score=209, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31732; Mature: 31601

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASAPAASTSARLLDGRRIAEELLDGLKSRVDARLAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQCTSEAELAALIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLSHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIEGAGV
ECCCCCCCCEEEECCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GIR
CCC
>Mature Secondary Structure 
TASAPAASTSARLLDGRRIAEELLDGLKSRVDARLAAGKARPGLAVVLVGGDPASSVYV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEE
RNKRRAAEKVGIEAFDYDLPQCTSEAELAALIDELNADPKIHGILIQLPLPGIPDANRLI
ECHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHH
QRIDPRKDVDGFHPQNVGHLALREFGLRPCTPRGIVTLLSHTDQPVRGRNATIVGVSNHV
HHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECHHCC
GRPMGLELLIAGCTVTSCHKFTPPDVLEASVRNADILVVAVGRPGLIPGEWVKPGAVVID
CCCCCEEEEEECCCEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEE
VGINRLDDGRLVGDVGFDAAAQRAAWITPVPGGVGPMTVATLMQNTIEAADAAGIEGAGV
ECCCCCCCCEEEECCCCCHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
GIR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]