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The map label for this gene is purR [H]

Identifier: 84623692

GI number: 84623692

Start: 2250179

End: 2251201

Strand: Direct

Name: purR [H]

Synonym: XOO_2035

Alternate gene names: 84623692

Gene position: 2250179-2251201 (Clockwise)

Preceding gene: 84623691

Following gene: 84623693

Centisome position: 45.55

GC content: 67.74

Gene sequence:

>1023_bases
ATGGCCAAGGGTGCGGTCACCATCAAAGATGTCGCTCGGGAAGCACAGGTCTCTGTCGCCACGGTGTCGCGTGCGCTCAA
TGGGCACGACAACGTCGCCGGACCGGTACGCCAGCAGGTGCTGGAAGTGGCCGAGCGACTGCGGTACAGCCCGCATGCGG
CCGCGCGCAGCCTGAGCAGCCGGCGCTCCCAGACCCTTGGCGTGGTGTTGCCTGATCTGTACGGCGAGTTCTTTTCCGAA
TTGATCCGTGGCATCGATGGCGCGGCGCGTGCGGCCGGCCAGCATCTGTTGGTGTCCAGCTATCACGGCGATCCGGAAGC
CCAGGGCAGGGCGCTGCGCTCGATGCGCGGGCGCGTCGATGGATTGCTGGTGCTGTCGCCGTATGCGGAGCAGCCGGGAT
TTCTGACCGACAATCTGCCGCAGTCGCTGCCGACGGTGCTGATCAATGCGCATCTGCCCGGTGCCGATTACCCGGTGCTG
AGCATCGACGACCACGCCGGCGCCATGGCCATGACCGAGCATCTGCTGCGCGCCGGACACCGCCGTATCGCCTTTATCGG
TGGCCCGGCGTTGAACTTCGATGCACGCGAACGCCTGCGCGGCTTTCGCGATGCGCTCGCTGCGTTTGGCGCGCAGGCGC
AGGGCGTAGAGTACCCGGGCGGCTTCGACGAAGCCTCCGGCCACCGCGCCGGTCTGGCGATGCTGGCCGACGGCCCGCTG
CCGGACGCGGTCTTCGCCGCCAACGACATGACCGCGCTCGGTTGCCTGTACGCGTTCGCCCAGGCAGATGTCTCGGTACC
CGGCGACGTGGCGCTGGCCGGGTTCGACGACATTCCGTTGGCCCGTTTTGTCCATCCGGCGCTGACCACCATGCGGGTGA
GCATCGCGCGGCTGGGCGAGCAGGCGATGACCCGGCTGATGCGCCTGGTCGAAATGCCCGGCCGCGACGACGGCATCCGT
CAATTGCTCACGCCCGAGCTGGTGATCCGGGCCTCGTGCGGTGCTGGCGAACCCGGTCAATAA

Upstream 100 bases:

>100_bases
AAGCGCTTTCACGCATGTGAAGACAGGGGTTTACGCCTTTGCATGAAAACGCTTACAGTCCGCGCCAGTTTTGCGAAGTC
GGTCGCGGAGGGCGACACGA

Downstream 100 bases:

>100_bases
CGTTTCCGGCCCTACGTTTTCGGGCTGCCGACCCTTTCGCGGACGTTCTGTCCGCTCCCGCTGCCCCCCAGTGGCTGGCA
CTGCCATAACAACAATCTGT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSE
LIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGGFDEASGHRAGLAMLADGPL
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVEMPGRDDGIR
QLLTPELVIRASCGAGEPGQ

Sequences:

>Translated_340_residues
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSE
LIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVL
SIDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGGFDEASGHRAGLAMLADGPL
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVEMPGRDDGIR
QLLTPELVIRASCGAGEPGQ
>Mature_339_residues
AKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSSRRSQTLGVVLPDLYGEFFSEL
IRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVDGLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLS
IDDHAGAMAMTEHLLRAGHRRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGGFDEASGHRAGLAMLADGPLP
DAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGEQAMTRLMRLVEMPGRDDGIRQ
LLTPELVIRASCGAGEPGQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=35.3658536585366, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=35.4515050167224, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790369, Length=343, Percent_Identity=33.5276967930029, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789068, Length=316, Percent_Identity=30.0632911392405, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=28.9808917197452, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=29.607250755287, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789456, Length=331, Percent_Identity=29.9093655589124, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787906, Length=304, Percent_Identity=27.9605263157895, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790715, Length=315, Percent_Identity=23.4920634920635, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786268, Length=285, Percent_Identity=23.1578947368421, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=22.7692307692308, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36086; Mature: 35954

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRSQTLGVVLPDLYGEFFSELIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLRAGH
EEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGGFDEASGHRAGLAMLADGPL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CCHHEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVEMPGRDDGIRQLLTPELVIRASCGAGEPGQ
HHHHHHHHHHHCCCCCHHHHHHHCHHHEEEECCCCCCCCC
>Mature Secondary Structure 
AKGAVTIKDVAREAQVSVATVSRALNGHDNVAGPVRQQVLEVAERLRYSPHAAARSLSS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
RRSQTLGVVLPDLYGEFFSELIRGIDGAARAAGQHLLVSSYHGDPEAQGRALRSMRGRVD
HHHHHHHEECHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCC
GLLVLSPYAEQPGFLTDNLPQSLPTVLINAHLPGADYPVLSIDDHAGAMAMTEHLLRAGH
EEEEECCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
RRIAFIGGPALNFDARERLRGFRDALAAFGAQAQGVEYPGGFDEASGHRAGLAMLADGPL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
PDAVFAANDMTALGCLYAFAQADVSVPGDVALAGFDDIPLARFVHPALTTMRVSIARLGE
CCHHEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QAMTRLMRLVEMPGRDDGIRQLLTPELVIRASCGAGEPGQ
HHHHHHHHHHHCCCCCHHHHHHHCHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA