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The map label for this gene is rlmB [H]

Identifier: 84623620

GI number: 84623620

Start: 2169850

End: 2170617

Strand: Direct

Name: rlmB [H]

Synonym: XOO_1963

Alternate gene names: 84623620

Gene position: 2169850-2170617 (Clockwise)

Preceding gene: 84623619

Following gene: 84623621

Centisome position: 43.92

GC content: 65.49

Gene sequence:

>768_bases
ATGAGCAAGCAGAATCAGTGGATCGTCGGCGTCAATGCTGTCGCCTCGTCCGTCGAGAACGACGCCGATAACGTGCGCGA
GGTATTGATCGAGGCCGGCAGCAAGAACCCGCGCCTCACCGAGATCGAAGAGCAGGCGCGCCGCAAGGGCATCGACGTGC
GCCGGGTCAACACCCAGGCGCTGGATGGCGTAGGCGGGCAGGTGCGTCATCAGGGCGTGGCCGCCCGCTATGCCGTGGCA
CGGCTATGGGCAGAGAATGAGCTGGAAGGCCTGGTCGAGGCAGCCGAAGGGCGCGCGCTGGTGCTGATCCTCGACGGCGT
ACAGGACCCGCACAACCTCGGCGCCTGCCTGCGTAGCGCGGCAGCCGCTGGCGTCACCGCGGTGGTGATTCCCAAGGACA
AGTCGGCCTCGGTGAATGCCACCGTGCGCAAGACCTCCGCTGGTGCGGCCGACCGCATTCCGGTGGTGGCGGTGACCAAT
CTGGCGCGCTGCCTGCGCGATCTGCAGAAGCAGGGCGTGTGGCTGTATGGCCTGGCCGGCGAAGCAGAGGCATCGTTGTA
CAGCGTGGATCTGCGCGGCAATGTCGGATTGGTGCTCGGTGGCGAGTCCGATGGCCTGCGCCGTCTCACGCGCGAGCACT
GCGACGGTCTGGTCAAGATTCCGATGCCGGGTGAGATCGAGAGCCTCAACGTATCGGTTGCGACCGGCGTGACCTTGTTC
GAAGTCGTACGCCAGCGCTTGGAGCAAGTTAGCCAACAGGAGGTTTGA

Upstream 100 bases:

>100_bases
TCATCGGCGTCACCCAGGCTCCCGCGCCCCCGCACCCCGGCCGTGACGTCACCGGCCGCAGGCCCTACCATTCCCCTTTA
TCTCTTCGTATGCCCGCGCA

Downstream 100 bases:

>100_bases
AGTGACACCAGATGAACGCAGAGCCTATGCGCAAACAATGTTTGCGCAGCACCCAGAGTTATTTCCGGATGATCGGCGGC
AGTCGATATTAAACGGGGTT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAVA
RLWAENELEGLVEAAEGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSASVNATVRKTSAGAADRIPVVAVTN
LARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGESDGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
EVVRQRLEQVSQQEV

Sequences:

>Translated_255_residues
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAVA
RLWAENELEGLVEAAEGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSASVNATVRKTSAGAADRIPVVAVTN
LARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGESDGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
EVVRQRLEQVSQQEV
>Mature_254_residues
SKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAVAR
LWAENELEGLVEAAEGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSASVNATVRKTSAGAADRIPVVAVTNL
ARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGESDGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLFE
VVRQRLEQVSQQEV

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=261, Percent_Identity=30.2681992337165, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=48.3471074380165, Blast_Score=237, Evalue=4e-64,
Organism=Escherichia coli, GI1788935, Length=154, Percent_Identity=35.0649350649351, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=282, Percent_Identity=27.3049645390071, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27215; Mature: 27084

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQA
CCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHH
LDGVGGQVRHQGVAARYAVARLWAENELEGLVEAAEGRALVLILDGVQDPHNLGACLRSA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVVIPKDKSASVNATVRKTSAGAADRIPVVAVTNLARCLRDLQKQGVWLYGLAG
HHCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
EAEASLYSVDLRGNVGLVLGGESDGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHH
EVVRQRLEQVSQQEV
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQA
CCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHH
LDGVGGQVRHQGVAARYAVARLWAENELEGLVEAAEGRALVLILDGVQDPHNLGACLRSA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVVIPKDKSASVNATVRKTSAGAADRIPVVAVTNLARCLRDLQKQGVWLYGLAG
HHCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
EAEASLYSVDLRGNVGLVLGGESDGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHH
EVVRQRLEQVSQQEV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]