Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 84623538

GI number: 84623538

Start: 2072863

End: 2074557

Strand: Direct

Name: asnB [H]

Synonym: XOO_1881

Alternate gene names: 84623538

Gene position: 2072863-2074557 (Clockwise)

Preceding gene: 84623537

Following gene: 84623542

Centisome position: 41.96

GC content: 63.19

Gene sequence:

>1695_bases
ATGTGTTCCATCTTTGGTATTTTCGGCCTGCAACCCGGTGACGACTTGCAGGCGCTACGCCGGCAGGCACTGGAATGTTC
GCAGCGGCAGCGCCATCGCGGGCCGGACTGGAGCGGCGTCTACGTCGATGCCGGGGCCATCCTGGTGCACGAGCGCCTGG
CCATCGTCGACCCGGCCGGCGGCTCGCAGCCGTTGCTGTCCGAAGACGGGCAGCTGGCGCTGGCGGTCAATGGCGAGATC
TACAACCATCGCGAACTCAAGGCCGAACTGCTGCAGCCGTACGCCTTCCAGACCGGCTCCGATTGCGAGGTGATCAATGC
GCTGTATCGCGAAGATGTGCCGGCCTTGTATCTCAATCGCCTCAACGGCATCTTTGCGTTTGCGCTGTGGGATAAGACGG
AAGGACGGGTGATCATCGCACGCGACCCGATCGGTGTGGTGCCGCTGTACTGGGGGCACGACCGCGAAGGCCGTCTGCGG
GTGGCGTCGGAATTGAAATCGCTGGTGGACGATTGCGCCGATGCGGCGCAGTTCCCTCCGGGCCATTGGTACGACAGCGC
CACCGGCGCGCTGAGCCGTTATTACGAGCGCGCCTGGCGCGAATATGCCGAGGTGGAAGGTGTGCAGGTGCAACTGCAGG
AATTGCGCGAAGCCTTCGAGCGTGCGGTGCATCGTCAACTGATGACCGACGTGCCGTACGGCGTGTTGTTGTCGGGCGGG
CTGGATTCCTCGTTGGTTGCAGCAGTTGCCGCGCGCTACGCCCGTCACCGCGTCGAAGAGAACGATACCACCGAAGCGTG
GTGGCCACGCCTGCATTCCTTCGCGATTGGCTTGAAGGGCTCGCCCGATTTGGCAGCAGCGGAAGTGGCCGCCGCTGCGC
TGGGCACCGTGCACCACGGCTTCGAATACACCTTCGAAGAGGGGCTGGATGCGTTGCCTGACGTCATTCGCCACATCGAA
ACCTACGACGTCACCACCATTCGCGCTTCCACACCGATGTTCTTATTGGCGCGGCGTATCAAGGCGATGGGCGTCAAGAT
GGTGCTCTCCGGCGAAGGCAGCGACGAGATCTTCGGCGGCTACCTGTATTTCCATAAGGCACCGAACGCGCGCGAATTCC
ATGAAGAGCTGGTGCGCAAGCTCGACGCGCTCAACAACTACGATTGCCTGCGCGCGAACAAGTCGATGATGGCCTGGGGC
GTGGAGCCGCGCGTGCCGTTCCTGGATCGCGAGTTCCTCGATGTGGCGATGCGCATGGACGCCAGTTTCAAAATGATCGA
CAAGACCAGCAGCGGCGCGGCGCGCATGGAAAAAGGCGTGCTGCGCGAAGCATTCGCAGGCTATCTGCCCGAGTCGATTC
TGTGGCGTCAGAAGGAGCAGTTCAGCGACGGCGTGGGCTATGGCTGGATCGACGGGCTGAAGGCGCATGCCGCAGCGAAC
GTCAGCGAACGCGAGCTCGCCGCTGCCGACCGACGCTACCCGGTCAACCCGCCGCAGACCAAGGAAGCGTATTTCTATCG
CACCTTGTTCGAGCAGTTTTTCCCCGGACAGTCGGCTGCGGAAACCGTGCCGGGAGGCAAGTCGATCGCGTGCTCGTCGC
CAACCGCGATCGCCTGGGATGCGAGTTTTGCCGCGATGGCAGACCCATCGGGCCGCGCCATTGCCGGCGTCCACGCGCAG
GCAATGGACTCGTAA

Upstream 100 bases:

>100_bases
AACCCGCATCGGCCGATGCGGGTTTTTTTATGGGTGTCATTTTCATGCAGCACGCTCTCTCACCGGCCGCCAACCCCAGC
AGTTATTCAGGAGTGTTTCC

Downstream 100 bases:

>100_bases
GGCTGCCGTAACTGCAGCGGCCGGCGTGCATTGCTGCACGGCCGGCCGCTGCGCTTGGTGCGCGTGTCTTATTGCTCCTG
GCGCACGCAATAGCCATAGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDAGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDVPALYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALSRYYERAWREYAEVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRVEENDTTEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFEEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDASFKMIDKTSSGAARMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAAAN
VSERELAAADRRYPVNPPQTKEAYFYRTLFEQFFPGQSAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
AMDS

Sequences:

>Translated_564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDAGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDVPALYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALSRYYERAWREYAEVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRVEENDTTEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFEEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDASFKMIDKTSSGAARMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAAAN
VSERELAAADRRYPVNPPQTKEAYFYRTLFEQFFPGQSAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
AMDS
>Mature_564_residues
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDAGAILVHERLAIVDPAGGSQPLLSEDGQLALAVNGEI
YNHRELKAELLQPYAFQTGSDCEVINALYREDVPALYLNRLNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLR
VASELKSLVDDCADAAQFPPGHWYDSATGALSRYYERAWREYAEVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
LDSSLVAAVAARYARHRVEENDTTEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHGFEYTFEEGLDALPDVIRHIE
TYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGGYLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWG
VEPRVPFLDREFLDVAMRMDASFKMIDKTSSGAARMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAAAN
VSERELAAADRRYPVNPPQTKEAYFYRTLFEQFFPGQSAAETVPGGKSIACSSPTAIAWDASFAAMADPSGRAIAGVHAQ
AMDS

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=556, Percent_Identity=36.8705035971223, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI168229252, Length=556, Percent_Identity=36.8705035971223, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI168229250, Length=556, Percent_Identity=36.8705035971223, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI296010848, Length=525, Percent_Identity=37.1428571428571, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI296010852, Length=459, Percent_Identity=37.037037037037, Blast_Score=249, Evalue=7e-66,
Organism=Homo sapiens, GI296010850, Length=459, Percent_Identity=37.037037037037, Blast_Score=249, Evalue=7e-66,
Organism=Escherichia coli, GI1786889, Length=561, Percent_Identity=59.5365418894831, Blast_Score=679, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=562, Percent_Identity=44.1281138790036, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71993933, Length=566, Percent_Identity=43.8162544169611, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17560178, Length=529, Percent_Identity=34.2155009451796, Blast_Score=288, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=38.41642228739, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321563, Length=593, Percent_Identity=49.9156829679595, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325403, Length=595, Percent_Identity=49.0756302521008, Blast_Score=525, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45553209, Length=577, Percent_Identity=45.2339688041594, Blast_Score=460, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62534; Mature: 62534

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDAGAILVHERLAIVDPAG
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCEEEECCCC
GSQPLLSEDGQLALAVNGEIYNHRELKAELLQPYAFQTGSDCEVINALYREDVPALYLNR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLRVASELKSLVDDCADAAQFPP
HCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHWYDSATGALSRYYERAWREYAEVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRVEENDTTEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
FEYTFEEGLDALPDVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCEEE
YLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCC
ASFKMIDKTSSGAARMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAAAN
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
VSERELAAADRRYPVNPPQTKEAYFYRTLFEQFFPGQSAAETVPGGKSIACSSPTAIAWD
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEECCCCCEEEEC
ASFAAMADPSGRAIAGVHAQAMDS
CCHHCCCCCCCCEEECCCHHHCCC
>Mature Secondary Structure
MCSIFGIFGLQPGDDLQALRRQALECSQRQRHRGPDWSGVYVDAGAILVHERLAIVDPAG
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCEEEECCCC
GSQPLLSEDGQLALAVNGEIYNHRELKAELLQPYAFQTGSDCEVINALYREDVPALYLNR
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LNGIFAFALWDKTEGRVIIARDPIGVVPLYWGHDREGRLRVASELKSLVDDCADAAQFPP
HCCEEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GHWYDSATGALSRYYERAWREYAEVEGVQVQLQELREAFERAVHRQLMTDVPYGVLLSGG
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
LDSSLVAAVAARYARHRVEENDTTEAWWPRLHSFAIGLKGSPDLAAAEVAAAALGTVHHG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCC
FEYTFEEGLDALPDVIRHIETYDVTTIRASTPMFLLARRIKAMGVKMVLSGEGSDEIFGG
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCEEE
YLYFHKAPNAREFHEELVRKLDALNNYDCLRANKSMMAWGVEPRVPFLDREFLDVAMRMD
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCC
ASFKMIDKTSSGAARMEKGVLREAFAGYLPESILWRQKEQFSDGVGYGWIDGLKAHAAAN
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
VSERELAAADRRYPVNPPQTKEAYFYRTLFEQFFPGQSAAETVPGGKSIACSSPTAIAWD
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEECCCCCEEEEC
ASFAAMADPSGRAIAGVHAQAMDS
CCHHCCCCCCCCEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]