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The map label for this gene is dapE [H]

Identifier: 84623537

GI number: 84623537

Start: 2071438

End: 2072568

Strand: Direct

Name: dapE [H]

Synonym: XOO_1880

Alternate gene names: 84623537

Gene position: 2071438-2072568 (Clockwise)

Preceding gene: 84623536

Following gene: 84623538

Centisome position: 41.93

GC content: 66.49

Gene sequence:

>1131_bases
ATGAACGATGTGCTGGATCTGACCTGCGATCTGATTGCACGCGCCTCGGTGACTCCCGAGGATGCTGGCTGCCAGGCACT
GCTTGCAGGGCGCTTGACTGCCGCGGGCTTCGCCTGCGAACACCTGCGCCTGGGCGAGGTCGACAATCTGTGGGCAACGC
ATGGCAGCGGCGCGCCGGTGCTGGTGTTGCTGGGGCATACCGATGTGGTACCGCCGGGCCCGCGCGAGGCCTGGACCAGC
GATCCGTTCGATCCGCAGATCCGCGACGGCGTGCTGTACGGTCGTGGCGTGGCTGATATGAAAGGCAGCGTCGCCGCGTT
CGTCGTCGCCGCGGAGCAGTTCGTCGCCGCGCATCGAGCGCATGCCGGCACGCTGGCAGTGTTGCTGACTTCGGACGAGG
AGGGCGATGCCATCGATGGCGTGCGCCGGGTCGCCAACCTCTTTCTCGAACGCGGACAGGCGATCGATTGGTGCATTACC
GGCGAGCCATCGTCCACCGAGCGCTTGGGCGATCTGTTGCGCGTTGGACGTCGCGGCAGCTTGTCCGGCACGCTCACCGT
CAAGGGGGTGCAGGGGCATGTGGCGTACCCACACAAGGCGCGCAACCCGATCCATCTGGCAGCGCCTGCGCTGGCCGAAC
TGGTCGCGCGGCAATGGGACGATGGCTTCGAAAGCTTCCCGCCGACCAGTCTGCAAGTCTCCAATATCCATGCCGGCACT
GGCGCCAATAACGTGATTCCCGGCGAGCTGCAGGTGGCCTTCAATCTGCGCTATACGCCGCACTGGGACGCGCCGCGCCT
GGAGGCGGAAATCACCGCGCTGCTCGATCGGCATGCGCTGGACTACGCGCTGCGCTGGCACCGCAGCGGCGAGCCGTTCT
ACACCCCGGAAGGGCGCTTGCGTAGCGTCGCGCGTGAGGTGTTGGGCGCGTTTGCCGGTGCGCCACCGGAAGAGAGCACC
GGCGGCGGCACCTCCGACGCGCGCTTCATCGCGCCATTGGGCGCGCAGTGCATCGAAGTCGGCCCGGTCAACGCCAGCAT
TCATCAGGTCGACGAGCATGTGCGCGTTGCCGATCTGCAAGCACTGCCAGCGCTGTATCGCACACTGATCGAGCGCTTGC
TGATCGAGTGA

Upstream 100 bases:

>100_bases
GAGCGTTTTGCGCTGCGCGCGCAGGCGATGCGCTGGCATGGCCCCGATGCTGCTCCAGCGATGCATAAATCAACCGTGTT
ATCACACAAGGCCTGACGAC

Downstream 100 bases:

>100_bases
GCGGGTCGCGTGCACACCGTCGTTGCCACTGAAACAGTTGCACCGGTGAAAACGAACGGTTAGCGTCAGCGACATGGTCA
TTCGCCTTGCCAACCTCAAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNDVLDLTCDLIARASVTPEDAGCQALLAGRLTAAGFACEHLRLGEVDNLWATHGSGAPVLVLLGHTDVVPPGPREAWTS
DPFDPQIRDGVLYGRGVADMKGSVAAFVVAAEQFVAAHRAHAGTLAVLLTSDEEGDAIDGVRRVANLFLERGQAIDWCIT
GEPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQVSNIHAGT
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGAFAGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRTLIERLLIE

Sequences:

>Translated_376_residues
MNDVLDLTCDLIARASVTPEDAGCQALLAGRLTAAGFACEHLRLGEVDNLWATHGSGAPVLVLLGHTDVVPPGPREAWTS
DPFDPQIRDGVLYGRGVADMKGSVAAFVVAAEQFVAAHRAHAGTLAVLLTSDEEGDAIDGVRRVANLFLERGQAIDWCIT
GEPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQVSNIHAGT
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGAFAGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRTLIERLLIE
>Mature_376_residues
MNDVLDLTCDLIARASVTPEDAGCQALLAGRLTAAGFACEHLRLGEVDNLWATHGSGAPVLVLLGHTDVVPPGPREAWTS
DPFDPQIRDGVLYGRGVADMKGSVAAFVVAAEQFVAAHRAHAGTLAVLLTSDEEGDAIDGVRRVANLFLERGQAIDWCIT
GEPSSTERLGDLLRVGRRGSLSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQVSNIHAGT
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRLRSVAREVLGAFAGAPPEEST
GGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQALPALYRTLIERLLIE

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=371, Percent_Identity=52.2911051212938, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790395, Length=205, Percent_Identity=33.6585365853659, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40239; Mature: 40239

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00605 ATPASE_C

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVLDLTCDLIARASVTPEDAGCQALLAGRLTAAGFACEHLRLGEVDNLWATHGSGAPV
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCE
LVLLGHTDVVPPGPREAWTSDPFDPQIRDGVLYGRGVADMKGSVAAFVVAAEQFVAAHRA
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
HAGTLAVLLTSDEEGDAIDGVRRVANLFLERGQAIDWCITGEPSSTERLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
LSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQVSNIHAGT
CCEEEEEEECCCCEECCCCCCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCC
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRL
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RSVAREVLGAFAGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHH
ALPALYRTLIERLLIE
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDVLDLTCDLIARASVTPEDAGCQALLAGRLTAAGFACEHLRLGEVDNLWATHGSGAPV
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCE
LVLLGHTDVVPPGPREAWTSDPFDPQIRDGVLYGRGVADMKGSVAAFVVAAEQFVAAHRA
EEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
HAGTLAVLLTSDEEGDAIDGVRRVANLFLERGQAIDWCITGEPSSTERLGDLLRVGRRGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
LSGTLTVKGVQGHVAYPHKARNPIHLAAPALAELVARQWDDGFESFPPTSLQVSNIHAGT
CCEEEEEEECCCCEECCCCCCCCEEEHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCC
GANNVIPGELQVAFNLRYTPHWDAPRLEAEITALLDRHALDYALRWHRSGEPFYTPEGRL
CCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
RSVAREVLGAFAGAPPEESTGGGTSDARFIAPLGAQCIEVGPVNASIHQVDEHVRVADLQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHH
ALPALYRTLIERLLIE
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]